STOCK Erc1^tm2.1Sud/J

Stock number: 015830
Product name: ELF-ELKS1 floxed
Research areas: Research Tools

Description

When crossed with a Cre recombinase-expressing strain, this floxed mutant strain is useful in eliminating tissue-specific expression of the Erc1 (ELKS/RAB6-interacting/CAST family member 1) gene.

Detailed description

A 5'UTR exon and the first coding exon of the Erc1 (ELKS/RAB6-interacting/CAST family member 1; ELKS1, CAST2) gene are flanked by loxP sites in these floxed mutant mice. Mice that are homozygous for this floxed allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Normal expression of the targeted gene is demonstrated by the floxed allele.

Development

A targeting vector was designed to flank a 5'UTR exon, the first coding exon, and an FRT-flanked neomycin cassette with loxP sites. The vector was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. The neomycin selection cassette was excised from chimeric animals though crosses with an Actb beta actin promoter Flp recombinase mouse originally made on a B6SJLF2 background, and possibly backcrossed to C57BL/6NCrl. This strain was maintained on a predominantly C57BL/6 and 129 mixed background by the donating laboratory.

Allele

Symbol: Erc1^tm2.1Sud
Name: targeted mutation 2.1, Thomas C Sudhof
MGI accession ID: MGI:4940381
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Erc1
Marker name: ELKS/RAB6-interacting/CAST family member 1
Chromosome: 6
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: A targeting vector was designed to flank a 5'UTR exon, the first coding exon, and an FRT-flanked neomycin cassette with loxP sites. Flp-mediated recombination removed the neo cassette.