This Myocd-Cre knock in mutation strain may be useful for generating conditional mutations for studying fate mapping of early skeletal muscle lineages.
Rhonda Bassel-Duby, University of Texas Southwestern
Eric N Olson, University of Texas Southwestern Medical
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Myocd | myocardin |
This strain expresses Cre recombinase from the endogenous Myocd locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs during early development, at embryonic day 7.5, in the developing heart, dorsal aorta, head mesenchyme and in the somites beginning at embryonic day 8.5.
Cre activity is also detected in skeletal muscle fibers and vasculature.
Heterozygous mutant mice are viable and fertile. The Jackson Laboratory has been unable to obtain homozygotes from heterozygous crosses.
A targeting vector containing cre coding sequence and a FRT site flanked PGK-neo cassette was used to disrupt exon 1 the targeted gene. The endogenous Myocd promoter drives expression of the Cre recombinase through the in-frame insertion of the cre coding sequence. The construct was electroporated into 129SvEvTac derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then crossed to FLPe recombinase expressing transgenic mice to remove the selection cassette. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre-mediated recombination occurs during early development, at embryonic day 7.5, in the developing heart, dorsal aorta, head mesenchyme and in the somites beginning at embryonic day 8.5. Cre activity is also detected in skeletal muscle fibers and vasculature. |
Allele Name | targeted mutation 1, Joseph M Miano |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Myocd-Cre |
Gene Symbol and Name | Myocd, myocardin |
Gene Synonym(s) | |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre-mediated recombination occurs during early development, at embryonic day 7.5, in the developing heart, dorsal aorta, head mesenchyme and in the somites beginning at embryonic day 8.5. Cre activity is also detected in skeletal muscle fibers and vasculature. |
Strain of Origin | Not Specified |
Chromosome | 11 |
Molecular Note | The coding region of Myocd exon 1 was replaced via homologous recombination with a construct containing a cre recombinase sequence and a Frt-flanked neo cassette, such that the endogenous Myocd promoter drove expression of cre. The neo cassette was subsequently removed by breeding the mice with Flpe trangenic animals. |
Mutations Made By | Rhonda Bassel-Duby, University of Texas Southwestern |
When maintaining a live colony, these mice can be bred as heterozygotes. The Jackson Laboratory has been unable to obtain homozygotes from heterozygous crosses.
When using the STOCK Myocdtm1(cre)Jomm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #014180 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Myocd<tm1(cre)Jomm> |
Frozen Mouse Embryo | STOCK Myocd<tm1(cre)Jomm>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Myocd<tm1(cre)Jomm>/J | $2595.00 |
Frozen Mouse Embryo | STOCK Myocd<tm1(cre)Jomm>/J | $3373.50 |
Frozen Mouse Embryo | STOCK Myocd<tm1(cre)Jomm>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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