STOCK Myocd^tm1(cre)Jomm/J

Stock number: 014180
Product name: Myocd-Cre
Strain synonyms: B6;129S6-Myocd^tm1(cre)Jomm/J
Research areas: Research Tools

Description

This Myocd-Cre knock-in mutation strain may be useful for generating conditional mutations for studying fate mapping of early skeletal muscle lineages.

Detailed description

This strain expresses Cre recombinase from the endogenous Myocd locus. When crossed with a strain containing loxP site flanked sequence of interest, Cre-mediated recombination results in tissue-specific deletion of the target. Recombination occurs during early development, at embryonic day 7.5, in the developing heart, dorsal aorta, head mesenchyme and in the somites beginning at embryonic day 8.5. Cre activity is also detected in skeletal muscle fibers and vasculature. Heterozygous mutant mice are viable and fertile. The Jackson Laboratory has been unable to obtain homozygotes from heterozygous crosses.

Development

A targeting vector containing cre coding sequence and a FRT site flanked PGK-neo cassette was used to disrupt exon 1 the targeted gene. The endogenous Myocd promoter drives expression of the Cre recombinase through the in-frame insertion of the cre coding sequence. The construct was electroporated into 129SvEvTac derived embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then crossed to FLPe recombinase expressing transgenic mice to remove the selection cassette. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.

Allele

Symbol: Myocd^tm1(cre)Jomm
Name: targeted mutation 1, Joseph M Miano
MGI accession ID: MGI:3849559
Generation method: Targeted
Attributes: Recombinase-expressing
Marker symbol: Myocd
Marker name: myocardin
Chromosome: 11
Strain of origin: Not Specified
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre-mediated recombination occurs during early development, at embryonic day 7.5, in the developing heart, dorsal aorta, head mesenchyme and in the somites beginning at embryonic day 8.5. Cre activity is also detected in skeletal muscle fibers and vasculature.
Molecular note: The coding region of Myocd exon 1 was replaced via homologous recombination with a construct containing a cre recombinase sequence and a Frt-flanked neo cassette, such that the endogenous Myocd promoter drove expression of cre. The neo cassette was subsequently removed by breeding the mice with Flpe trangenic animals.