These knock-out mice are lacking exons 1-2 of the Batf3 gene, abolishing gene function. This strain may be useful for studying CD8 deficiency, antigen cross-presentation, and CD8- T cell responses against viral infection and responses to tumor challenge.
Dr. Kenneth Murphy, Washington University School of Medicine
These Batf3-/- mutant mice are lacking exons 1-2 of the basic leucine zipper transcription factor, ATF-like 3 (Batf3) gene, abolishing gene function. Mice that are homozygous for this allele are viable and fertile. Batf3 is highly expressed in CD11c+ CD8α+ conventional dendritic cells (cDCs), with low to absent expression in other immune cells.The deletion of Batf3 prevents development of splenic CD8α+ cDCs, which are important for cross-presentation during infections. Lymph nodes and thymi of Batf3-/- mice lack CD8α+ DCs, and DCs generated from Batf3-/- bone marrow (BM) and spleens are deficient in Toll-like receptor (TLR) 3-induced interleukin (IL)-12 production. These mice also lack CD103+CD11b- DCs in the lung, intestine, mesenteric lymph nodes (MLNs), dermis, and skin-draining lymph nodes. Exhibiting defects in CD8+T cell response priming, including lack of virus-specific responses, results in the inability to reject highly immunogenic syngeneic tumors. This strain may be useful for studying CD8 deficiency, antigen cross-presentation, and CD8- T cell responses against viral infection and responses to tumor challenge.
A targeting vector was designed to replace exons 1-2 of the basic leucine zipper transcription factor, ATF-like 3 (Batf3) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived MC50 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to 129SvEv females to establish a colony. These mice were subsequently bred to
BALB/c-Tg(CMV-cre)1Cgn/J mice (Stock No. 003465) to remove the neo cassette. These mice were backcrossed to BALB/c mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ inbred mice (Stock No. 000651) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Kenneth M Murphy|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Batf3, basic leucine zipper transcription factor, ATF-like 3|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exons 1-2 were replaced by a floxed neomycin cassette. Cre-mediated recombination removed the neo cassette. Gene inactivation was confirmed by a lack of protein detected by immunoblot analysis of Th1 CD4+ T cells.|
|Mutations Made By|| |
Dr. Kenneth Murphy, Washington University School of Medicine
When maintaining a live colony, homozygous mice may be bred together.
When using the Batf3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #013756 in your Materials and Methods section.
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