These knock-out mice are lacking exons 1-2 of the Batf3 gene, abolishing gene function. This strain may be useful for studying CD8 deficiency, antigen cross-presentation, and CD8- T cell responses against viral infection and responses to tumor challenge.
Dr. Kenneth Murphy, Washington Univ School of Medicine
These Batf3-/- mutant mice are lacking exons 1-2 of the basic leucine zipper transcription factor, ATF-like 3 (Batf3) gene, abolishing gene function. Mice that are homozygous for this allele are viable and fertile. Batf3 is highly expressed in CD11c+ CD8α+ conventional dendritic cells (cDCs), with low to absent expression in other immune cells.The deletion of Batf3 prevents development of splenic CD8α+ cDCs, which are important for cross-presentation during infections. Lymph nodes and thymi of Batf3-/- mice lack CD8α+ DCs, and DCs generated from Batf3-/- bone marrow (BM) and spleens are deficient in Toll-like receptor (TLR) 3-induced interleukin (IL)-12 production. These mice also lack CD103+CD11b- DCs in the lung, intestine, mesenteric lymph nodes (MLNs), dermis, and skin-draining lymph nodes. Exhibiting defects in CD8+T cell response priming, including lack of virus-specific responses, results in the inability to reject highly immunogenic syngeneic tumors. This strain may be useful for studying CD8 deficiency, antigen cross-presentation, and CD8- T cell responses against viral infection and responses to tumor challenge.
A targeting vector was designed to replace exons 1-2 of the basic leucine zipper transcription factor, ATF-like 3 (Batf3) gene with a loxP-flanked neomycin resistance (neo) cassette. The construct was electroporated into 129S6/SvEvTac-derived MC50 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and resulting chimeric males were bred to 129SvEv females to establish a colony. These mice were subsequently bred to
BALB/c-Tg(CMV-cre)1Cgn/J mice (Stock No. 003465) to remove the neo cassette. These mice were backcrossed to BALB/c mice for at least 10 generations. Upon arrival at The Jackson Laboratory, mice were bred to BALB/cJ inbred mice (Stock No. 000651) for at least one generation to establish the colony.
|Allele Name||targeted mutation 1, Kenneth M Murphy|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Batf3tm1Kmm; targeted mutation 1, Kenneth M Murphy|
|Gene Symbol and Name||Batf3, basic leucine zipper transcription factor, ATF-like 3|
|Gene Synonym(s)||SNFT; 9130211I03Rik; JDP1; JUNDM1; RIKEN cDNA 9130211I03 gene; 9130211I03Rik; Jdp1|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||Exons 1-2 were replaced by a floxed neomycin cassette. Cre-mediated recombination removed the neo cassette. Gene inactivation was confirmed by a lack of protein detected by immunoblot analysis of Th1 CD4+ T cells.|
|Mutations Made By|| |
Dr. Kenneth Murphy, Washington Univ School of Medicine
When maintaining a live colony, homozygous mice may be bred together.
When using the Batf3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #013756 in your Materials and Methods section.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON’s prior written authorization.
MICE, PRODUCTS AND SERVICES ARE PROVIDED "AS IS". JACKSON EXTENDS NO WARRANTIES OF ANY KIND, EITHER EXPRESS, IMPLIED, OR STATUTORY, WITH RESPECT TO MICE, PRODUCTS OR SERVICES, INCLUDING ANY IMPLIED WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE, OR ANY WARRANTY OF NON-INFRINGEMENT OF ANY PATENT, TRADEMARK, OR OTHER INTELLECTUAL PROPERTY RIGHTS.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON’s MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.