These knockin mutant mice possess a floxed GATA4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA; cre-mediated excision activates AP expression. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.
William T Pu, Children's Hospital Boston, Harvard MS
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Reporter, Inserted expressed sequence) | Gata4 | GATA binding protein 4 |
Homozygotes: Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Cre-mediated excision of Gata4 is coupled with alkaline phosphatase expression under the control of the endogenous Gata4 regulatory elements. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.
Heterozygote: Normal
A targeting vector containing a loxP-flanked Gata4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA and an FRT-flanked PGKneo cassette was inserted into the 5' UTR of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and SJL mixed background Tg(ACTFLPe)9205Dym mice to remove the neomycin cassette. To remove the FLP transgene, mice were mated with outbred Black Swiss mice and then intercrossed to establish a homozygous colony. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Expressed Gene | Gata4, GATA binding protein 4, mouse, laboratory |
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Site of Expression |
Allele Name | targeted mutation 2, William T Pu |
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Allele Type | Targeted (Reporter, Inserted expressed sequence) |
Allele Synonym(s) | Gata4flap |
Gene Symbol and Name | Gata4, GATA binding protein 4 |
Gene Synonym(s) | |
Expressed Gene | Gata4, GATA binding protein 4, mouse, laboratory |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 14 |
Molecular Note | Exon 2 was replaced with a floxed full-length Gata4 cDNA with a transcription STOP sequence followed by an alkaline phosphatase gene with a 3' frt site. An frt-flanked neo cassette used for selection purposes was removed by germ line, flp-mediated recombination. Cre mediated recombination is necessary to activate alkaline phospatase expression. |
Mutations Made By | William Pu, Children's Hospital Boston, Harvard MS |
While maintaining a live colony, these mice are bred as homozygotes.
When using the Gata4flap mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32883 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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