Overview

Also Known As:Gata4^flap

These knockin mutant mice possess a floxed GATA4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA; cre-mediated excision activates AP expression. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.

Donating Investigator

William T Pu, Children's Hospital Boston, Harvard MS

Genetic overview

Genetic Background	Generation

Gata4^{tm2(Gata4)Wtp}

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Inserted expressed sequence)	Gata4	GATA binding protein 4

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Research Applications

Research Tools

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Details

Detailed Description

Homozygotes: Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Cre-mediated excision of Gata4 is coupled with alkaline phosphatase expression under the control of the endogenous Gata4 regulatory elements. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.

Heterozygote: Normal

Development

A targeting vector containing a loxP-flanked Gata4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA and an FRT-flanked PGKneo cassette was inserted into the 5' UTR of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and SJL mixed background Tg(ACTFLPe)9205Dym mice to remove the neomycin cassette. To remove the FLP transgene, mice were mated with outbred Black Swiss mice and then intercrossed to establish a homozygous colony. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Expression Data

Expressed Gene	Gata4, GATA binding protein 4, mouse, laboratory
Site of Expression

Control Suggestions

Approximate Controls

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Ma Q; Zhou B; Pu WT. 2008. Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity. Dev Biol 323(1):98-104PubMed: 18775691 MGI: J:141152
Zhou B; Ma Q; Rajagopal S; Wu SM; Domian I; Rivera-Feliciano J; Jiang D; von Gise A; Ikeda S; Chien KR; Pu WT. 2008. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454(7200):109-13PubMed: 18568026 MGI: J:137426

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Genetics

Gata4^{tm2(Gata4)Wtp}

Allele Symbol: Gata4^{tm2(Gata4)Wtp}

Allele Name	targeted mutation 2, William T Pu
Allele Type	Targeted (Reporter, Inserted expressed sequence)
Allele Synonym(s)	Gata4^flap
Gene Symbol and Name	Gata4, GATA binding protein 4
Gene Synonym(s)
Expressed Gene	Gata4, GATA binding protein 4, mouse, laboratory
Strain of Origin	129S6/SvEvTac
Chromosome	14
Molecular Note	Exon 2 was replaced with a floxed full-length Gata4 cDNA with a transcription STOP sequence followed by an alkaline phosphatase gene with a 3' frt site. An frt-flanked neo cassette used for selection purposes was removed by germ line, flp-mediated recombination. Cre mediated recombination is necessary to activate alkaline phospatase expression.
Mutations Made By	William Pu, Children's Hospital Boston, Harvard MS

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - loxP-flanked Sequences: Test/Reporter
- Cardiovascular Research
  - Cre-lox System

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Gata4^{tm2(Gata4)Wtp}/Gata4⁺ Tg(Myh6-cre)2182Mds/0
involves: 129S6/SvEvTac * FVB/N

mammalian phenotype

no phenotypic analysis
- Normal - used for cell lineage tracing

References

Ma Q; Zhou B; Pu WT. 2008. Reassessment of Isl1 and Nkx2-5 cardiac fate maps using a Gata4-based reporter of Cre activity. Dev Biol 323(1):98-104PubMed: 18775691 MGI: J:141152
Zhou B; Ma Q; Rajagopal S; Wu SM; Domian I; Rivera-Feliciano J; Jiang D; von Gise A; Ikeda S; Chien KR; Pu WT. 2008. Epicardial progenitors contribute to the cardiomyocyte lineage in the developing heart. Nature 454(7200):109-13PubMed: 18568026 MGI: J:137426

Additional - Gata4^{tm2(Gata4)Wtp} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Gata4
- Genotyping resources and troubleshooting
Breeding Considerations

While maintaining a live colony, these mice are bred as homozygotes.
- Additional Breeding and Husbandry Support
Citation
When using the Gata4^flap mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32883 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

See MMRRC for Additional Conditions of Distribution

Questions about Terms of Use

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:Gata4flap

Donating Investigator

Genetic overview

Research Applications

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Gata4tm2(Gata4)Wtp

Allele Symbol: Gata4tm2(Gata4)Wtp

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gata4tm2(Gata4)Wtp/Gata4+ Tg(Myh6-cre)2182Mds/0involves: 129S6/SvEvTac * FVB/N

mammalian phenotype

References

Additional - Gata4tm2(Gata4)Wtp related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

Also Known As:Gata4^flap

Gata4^{tm2(Gata4)Wtp}

Allele Symbol: Gata4^{tm2(Gata4)Wtp}

Genotype: Gata4^{tm2(Gata4)Wtp}/Gata4⁺ Tg(Myh6-cre)2182Mds/0
involves: 129S6/SvEvTac * FVB/N

Additional - Gata4^{tm2(Gata4)Wtp} related