STOCK Gata4^{tm2(Gata4)Wtp}/Mmjax

Stock number: 013145
Product name: Gata4^flap
Research areas: Research Tools

Description

These knockin mutant mice possess a floxed GATA4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA; cre-mediated excision activates AP expression. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.

Detailed description

Homozygotes: Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice show no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Cre-mediated excision of Gata4 is coupled with alkaline phosphatase expression under the control of the endogenous Gata4 regulatory elements. This mutant mouse strain may be useful in studies of cell lineage tracing in the developing and adult heart.

Heterozygote: Normal

Development

A targeting vector containing a loxP-flanked Gata4 cDNA with a transcriptional stop cassette followed by an alkaline phosphatase cDNA and an FRT-flanked PGKneo cassette was inserted into the 5' UTR of the targeted gene. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Chimeric animals were crossed to C57BL/6 and SJL mixed background Tg(ACTFLPe)9205Dym mice to remove the neomycin cassette. To remove the FLP transgene, mice were mated with outbred Black Swiss mice and then intercrossed to establish a homozygous colony. Upon arrival, mice were bred to C57BL/6J for at least 1 generation to establish the colony.

Allele

Symbol: Gata4^{tm2(Gata4)Wtp}
Name: targeted mutation 2, William T Pu
MGI accession ID: MGI:3801683
Generation method: Targeted
Attributes: Reporter; Inserted expressed sequence
Marker symbol: Gata4
Marker name: GATA binding protein 4
Chromosome: 14
Strain of origin: 129S6/SvEvTac
Expressed genes: Gata4,GATA binding protein 4,mouse, laboratory
Molecular note: Exon 2 was replaced with a floxed full-length Gata4 cDNA with a transcription STOP sequence followed by an alkaline phosphatase gene with a 3' frt site. An frt-flanked neo cassette used for selection purposes was removed by germ line, flp-mediated recombination. Cre mediated recombination is necessary to activate alkaline phospatase expression.