This null allele of adducin 3 has an EGFP reporter inserted into exon 2, the first coding exon. Homozygotes are viable and fertile.
Dr. Luanne Peters, The Jackson Laboratory
This null allele with EGFP reporter permits assessment of adducin 3 promoter expression or phenotypic characterization of null homozygotes. Weak EGFP expression was found throughout the embryo and extraembryonic membranes at E9, the earliest time assessed, through E16, the latest, with the highest level of expression found in the developing heart tube. In adult tissues EGFP was found expressed in all lymphoid and myeloid cells of the bone marrow, spleen, and blood, but not in circulating red blood cells or early erythroblast subsets in bone or spleen. Homozygous null mice are viable and fertile.
This null reporter allele was generated by the insertion of an EGFP-pA cassette into exon 2, the first coding exon, and an FRT-flanked neomycin selection cassette into the downstream intron. This was done in 129P2/OlaHsd-derived ES cells and the neomycin selection cassette was excised by breeding to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J yielding the tm2.1Llp allele. This line was subsequently bred to C57BL/6J, repeatedly backcrossed and sperm was cryopreserved from heterozygous males at approximately generation N10.
|Allele Name||targeted mutation 2.1, Luanne Peters|
|Allele Type||Targeted (Reporter, Null/Knockout)|
|Gene Symbol and Name||Add3, adducin 3 (gamma)|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||An EGFP-pA cassette was inserted into exon 2, the first coding exon, and an FRT-flanked neomycin selection cassette was inserted into the downstream intron, which was excised by breeding to a mouse with germline expression of FLP1 recombinase, resulting in a null allele with expressed reporter and no neomycin selection sequence.|