These clock fluorescent reporter (mPeriod1-Venus) mutant mice have widespread expression in multiple brain regions paralleling Per1 expression and recapitulating Per1 rhythmic and light-inducible expression in the SCN. This mutant mouse strain may be useful for in vivo imaging to monitor circadian clock timing.
Karl Obrietan, Ohio State University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Reporter) |
Mice that are hemizygous for the clock fluorescent reporter transgene are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. The mPeriod1-Venus transgene has widespread expression in multiple brain regions (high expression in: suprachiasmatic nuclei (SCN), Purkinje cells, cerebellum, cerebral cortex and hippocampus) and parallels Per1 expression. In addition, the transgene recapitulates Per1 rhythmic and light-inducible expression in the SCN. This mutant mouse strain may be useful for in vivo imaging to monitor circadian clock timing.
A bacterial articificial chromosone (BAC) #RP24-277K16 containing the mouse Per1 gene was modified by the introduction of a Venus (yellow fluorescent protein)-NLS-PEST fusion gene. To improve the kinetics of YFP, the PEST domain of the mouse ornithine carboxylase gene was added to the penultimate codon of Venus and a nuclear localization sequence (NLS) was cloned in-frame between the Venus coding sequence and the PEST domain. The fusion gene contains an hCMV promoter and a SV40 polyadenylation site. The BAC was microinjected into the pronuclei of oocytes from FVB/N females. Founder line 33 was established and bred to C57BL/6J mice to generate the colony. Mice were subsequently backcrossed to C57BL/6J mice for three generations. mice were bred to C57BL/6J for at least 1 generation to establish the colony.
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
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Site of Expression | Widespread expression in multiple brain regions. |
Allele Name | transgene insertion 33, Karl Obrietan |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | mPer1-Venus; mPer1-Venus founder line no. 33 |
Gene Symbol and Name | Tg(Per1-Venus)33Obr, transgene insertion 33, Karl Obrietan |
Gene Synonym(s) | |
Promoter | Per1, period circadian clock 1, mouse, laboratory |
Expressed Gene | YFP, Yellow Fluorescent Protein, jellyfish |
Site of Expression | Widespread expression in multiple brain regions. |
Strain of Origin | FVB/N |
Chromosome | UN |
General Note | The data reported were obtained using transgenic line 33 and were "consistent with results obtained from the other three transgenic lines," 7, 13, 33 and 4126. (J:150729) |
Molecular Note | The transgene comprises bacterial artificial chromosome (BAC) RP24-277K16 containing the mouse period 1 (Per1) gene into which a Venus-NLS-PEST fusion gene has been inserted. Venus is a yellow fluorescent protein enhanced by the introduction of five point mutations. To improve the protein's kinetics, the PEST domain of the mouse ornithine decarboxylase gene was introduced following the penultimate codon of the gene. A nuclear localization signal (NLS) was cloned in-frame between the Venus coding sequence and the PEST domain. The sequence encoding the fusion protein is preceded by a human cytomegalovirus (hCMV) promoter and followed by an SV40 polyadenylation signal. Its robust expression in a population of cells in the suprachiasmatic nuclei (SCN) of the hypothalamus is cyclic and light-inducible; it is also widely expressed in neuronal and non-neuronal cells of brain regions outside the SCN. |
Mutations Made By | Karl Obrietan, Ohio State University |
While maintaining a live colony, these mice are bred as hemizygotes. The donating investigator has not tried to maintain this as a homozygote colony.
When using the B6.FVB-Tg(Per1-Venus)33Obr/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32820 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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