This KEPI KO mutant mouse strain may be useful in studies of morphine analgesia and tolerance.
George R. Uhl, NIDA-IRP/NIH
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Ppp1r14c | protein phosphatase 1, regulatory inhibitor subunit 14C |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. The Donating Investigator reports that the expected number of homozygotes are produced in heterozygous crosses. No gene product (mRNA) is detected by RT-PCR analysis of brain tissue from homozygotes. SNP analysis revealed that the Oprm1 allele variant, which is located close to the targeted gene, is from C57BL/6. Homozygotes exhibit increased phosphatase 1 activity in the thalamus. Homozygotes develop faster morphine tolerance in a hot plate assay ("supraspinal" nociceptive response assessment) and a rightward shift in dose response curve for morphine reward in conditioned place preference when compared to wildtype controls.
A neo cassette-containing targeting vector was used to disrupt exon 1, which contains the initiation codon and the potential PP1 docking motif. The construct was electroporated into 126S6/SvEvTac derived MC1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6J blastocysts. The resulting heterozygous chimeric animals were intercrossed to generate homozygotes. SNP analysis revealed that the Oprm1 allele variant, which is located close to the targeted gene, is from C57BL/6. Upon arrival at The Jackson Laboratory, the mice were crossed to C57BL/6J (Stock No. 000664) at least once to establish the colony.
Allele Name | targeted mutation 1, George R Uhl |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Ppp1r14c, protein phosphatase 1, regulatory inhibitor subunit 14C |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 10 |
General Note | The original line on a mixed 129S6/SvEvTac and C57BL/6 background was discontinued in favor of a recombinant line containing regions adjacent to the targeted allele from C57BL/6 (J:159561). |
Molecular Note | Exon 1 was replaced with a neo cassette. RT-PCR confirmed the absence of transcript expression. |
Mutations Made By | George Uhl, NIDA-IRP/NIH |
When maintaining a live colony, these mice can be bred as homozygotes. The Donating Investigator reports that the expected number of homozygotes are produced in heterozygous crosses.
When using the B6;129S6-Ppp1r14ctm1Uhl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #013041 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or wildtype for Ppp1r14c<tm1Uhl> |
Frozen Mouse Embryo | B6;129S6-Ppp1r14c<tm1Uhl>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S6-Ppp1r14c<tm1Uhl>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S6-Ppp1r14c<tm1Uhl>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S6-Ppp1r14c<tm1Uhl>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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