129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. They also have a high incidence of spontaneous testicular teratomas.
Elizabeth MC Fisher, UCL Institute of Neurology
Historically, the 129 inbred mice are known for the high incidence of spontaneous testicular teratomas, though the incidence differs between substrains.(1-3% in 129 parental substrains; 30% in teratoma substrains.) More recently, 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. There is major genetic variation within the 129 "family", which has led to an update of the nomenclature and a division of the substrains into three major groups: parental substrains (129P), steel substrains (129S) and "teratoma" substrains (129T). Investigators using 129 substrains for targeted mutagenesis should be careful in the selection of the appropriate 129 substrain to match the embryonic stem cell line.
Dr. Leroy Stevens (The Jackson Laboratory) developed the Steel 129 substrain (129/Sv) in the 1960s. 129/Sv was transferred to Martin Evans (Cambridge) who introduced the Gpic allele from a 101 strain background. 129/SvEv-Gpic was transferred to the MRC Mammalian Development Unit in 1986 and then to MRC-NIMR in 1988. The strain was subsequently designated 129S8/SvEvNimr. It has been maintained by sibling matings for more than 20 years. The Jackson Laboratory obtained the strain in 2010.
|Allele Synonym(s)||Disc1129S6; Disc1delta6|
|Gene Symbol and Name||Disc1, disrupted in schizophrenia 1|
|Strain of Origin||various|
|General Note||This deletion appears in multiple strains of the 129 superfamily, 101/RI, BTBR T+ tf/J, LP/J, FVB/NJ, SJL/J, SWR/J and DDY/JclSidSeyFrkJ (J:111837, J:195189).|
|Molecular Note||A 25 bp deletion in exon 6 causes a frame shift in the reading frame, resulting in 13 novel amino acids and a premature stop codon in exon 7.|
When maintaining a live colony, this strain is maintained by sibling matings.
When using the 129S8 Inbred mouse strain in a publication, please include JAX stock #012809 in your Materials and Methods section.