129S8/SvEvNimrJ

Stock number: 012809
Product name: 129S8 Inbred

Description

129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. They also have a high incidence of spontaneous testicular teratomas.

Detailed description

Historically, the 129 inbred mice are known for the high incidence of spontaneous testicular teratomas, though the incidence differs between substrains.(1-3% in 129 parental substrains; 30% in teratoma substrains.) More recently, 129 mice are widely used in the production of targeted mutations due to the availability of multiple embryonic stem cell lines derived from them. There is major genetic variation within the 129 "family", which has led to an update of the nomenclature and a division of the substrains into three major groups: parental substrains (129P), steel substrains (129S) and "teratoma" substrains (129T). Investigators using 129 substrains for targeted mutagenesis should be careful in the selection of the appropriate 129 substrain to match the embryonic stem cell line.

Development

Dr. Leroy Stevens (The Jackson Laboratory) developed the Steel 129 substrain (129/Sv) in the 1960s. 129/Sv was transferred to Martin Evans (Cambridge) who introduced the Gpi^c allele from a 101 strain background. 129/SvEv-Gpi^c was transferred to the MRC Mammalian Development Unit in 1986 and then to MRC-NIMR in 1988. The strain was subsequently designated 129S8/SvEvNimr. It has been maintained by sibling matings for more than 20 years. The Jackson Laboratory obtained the strain in 2010.

Allele

Symbol: Disc1^del
Name: deletion
MGI accession ID: MGI:3623217
Generation method: Spontaneous
Synonyms: Disc1^129S6; Disc1^delta6
Marker symbol: Disc1
Marker name: disrupted in schizophrenia 1
Marker synonyms: C1orf136; SCZD9
Chromosome: 8
Strain of origin: various
Molecular note: A 25 bp deletion in exon 6 causes a frame shift in the reading frame, resulting in 13 novel amino acids and a premature stop codon in exon 7.
General note: This deletion appears in multiple strains of the 129 superfamily, 101/RI, BTBR T⁺ tf/J, LP/J, FVB/NJ, SJL/J, SWR/J and DDY/JclSidSeyFrkJ (J:111837, J:195189).