Overview

Tamoxifen-induced, Cre-mediated targeted deletions in tissues such as myogenic progenitor cells and adult myogenic satellite cells can be created upon crossing these Pax7^CE mutant mice with a strain carrying a floxed gene of interest.

Donating Investigator

Chen-Ming Fan, Carnegie Institution of Washington

Genetic overview

Genetic Background	Generation

Pax7^{tm2.1(cre/ERT2)Fan}

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing, Inducible, Null/Knockout)	Pax7	paired box 7

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Research Applications

Research Tools

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Base Price

Starting at:

$2,595.00 Domestic price Cryo Recovery

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Details

Detailed Description

Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Only approximately 10% of homozygotes live to adulthood. Surviving adult homozygotes are approximately half the size of heterozygote animals. The fertility of homozygotes has not been tested. In contrast to B6.129S-Pax7^{tm1(cre/ERT2)Gaka}/J mice, which express a functional PAX7 gene product, no PAX7 protein is detected in 10 day old homozygotes. The Ds-Red is not detectable by immunostaining or FACS. When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions in tissues such as myogenic progenitor cells and adult myogenic satellite cells.

Development

A targeting vector was used to insert loxP sites flanking exon 2. Exon 2 was excised via transient Cre mediated recombination to generate the Pax7^tm1.1Fan allele. Exon 1 in the Pax7^tm1.1Fan allele was replaced with a targeting vector containing sequence encoding Cre-ERT2, IRES-DsRed and an FRT flanked PGK-neo cassette. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl⁺ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice expressing FLP recombinase under the control of an Actin promoter (on the C57BL/6 background) to remove the neo selection cassette. The mice were then backcrossed to C57BL/6J for 2 generations. Upon arrival at The Jackson Laboratory the mice were crossed to C57BL/6J once to establish the colony.

Expression Data

Expressed Gene	RFP, Red Fluorescent Protein, coral
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions in tissues such as myogenic progenitor cells and adult myogenic satellite cells.

Control Suggestions

Wild-type from the colony

Additional Information

Considerations for Choosing Controls

Selected References

Lepper C; Conway SJ; Fan CM. 2009. Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements. Nature 460(7255):627-31PubMed: 19554048 MGI: J:150962

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Genetics

Pax7^{tm2.1(cre/ERT2)Fan}

Allele Symbol: Pax7^{tm2.1(cre/ERT2)Fan}

Allele Name	targeted mutation 2.1, Chen-Ming Fan
Allele Type	Targeted (Recombinase-expressing, Inducible, Null/Knockout)
Allele Synonym(s)	targeted mutation 2.1, Chen-Ming Fan; Pax7^{tm2.1(cre/ERT2)Fan}
Gene Symbol and Name	Pax7, paired box 7
Gene Synonym(s)	Pax-7; HUP1; PAX7B; RMS2; Pax-7; RGD1564360
Expressed Gene	RFP, Red Fluorescent Protein, coral
Expressed Gene	cre/ERT2, Cre recombinase and estrogen receptor 1 (human) fusion gene,
Site of Expression	When crossed with a strain containing a loxP site flanked sequence of interest, the offspring are useful for generating tamoxifen-induced, Cre-mediated targeted deletions in tissues such as myogenic progenitor cells and adult myogenic satellite cells.
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	4
Molecular Note	Beginning with the Pax7^tm1.2Sjc allele in which exon 2 was knocked out leaving a single loxP site, part of exon 1 was replaced with a cre/ERT2 (ESR1) fusion followed by an IRES-DsRed and an frt flanked neo cassette. Germ line, flp mediated recombination removed the neo cassette. The absence of full-length Pax7 protein expression was confirmed by western blot analysis on P10 tibialis anterior muscle extracts. Expression of the fusion cre/ERT2 protein was confirmed by a cross with a reporter line. DsRed fluorescence was not detectable by epifluorescence nor was DsRed protein by immunostaining.
Mutations Made By	Chen-Ming Fan, Carnegie Institution of Washington

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Cre-lox System
  - Cre Recombinase Expression
  - Cre Recombinase Expression: Inducible

Mammalian Phenotype Terms by Genotype

References

Lepper C; Conway SJ; Fan CM. 2009. Adult satellite cells and embryonic muscle progenitors have distinct genetic requirements. Nature 460(7255):627-31PubMed: 19554048 MGI: J:150962

Additional - Pax7^{tm2.1(cre/ERT2)Fan} related

Technical Support

Contact Technical Support

Genotyping Protocols
- Separated PCR: Pax7^{tm2.1(cre/ERT2)Fan}alternate2
- High Resolution Melting: Pax7^{tm2.1(cre/ERT2)Fan}alternate2
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as heterozygotes. Only approximately 10% of homozygotes live to adulthood. Surviving adult homozygotes are approximately half the size of heterozygote animals. The fertility of homozygotes has not been tested.
- Additional Breeding and Husbandry Support
Citation
When using the B6;129-Pax7^{tm2.1(cre/ERT2)Fan}/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #012476 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous or wildtype for Pax7<tm2.1(cre/ERT2)Fan>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Pax7tm2.1(cre/ERT2)Fan

Allele Symbol: Pax7tm2.1(cre/ERT2)Fan

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

References

Additional - Pax7tm2.1(cre/ERT2)Fan related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Pax7^{tm2.1(cre/ERT2)Fan}

Allele Symbol: Pax7^{tm2.1(cre/ERT2)Fan}

Additional - Pax7^{tm2.1(cre/ERT2)Fan} related