The caBmpr1a transgene has a floxed Tau-β-galactosidase gene followed by a constitutively active mutant form of human BMP receptor type IA (caBmpr1a Q227D), an IRES sequence, and the EGFP coding sequence. Transgenic mice have widespread expression of lacZ prior to exposure to Cre recombinase. When bred with mice that express Cre recombinase, offspring will have the floxed-lacZ gene deleted and subsequent expression of caBmpr1a and EGFP in the cre-expressing cells. These mice may be useful for studying BMP signaling in tissues where Cre expresses such as cartilage and muscle, osteoclastogenesis (via RANKL-OPG pathway and/or canonical Wnt signaling), age dependant bone loss, osteoporosis, chondrodysplasia, fibrodysplasia ossificans progressive, and mineralization in muscle/skeletal development.
Manas Ray, NIEHS
Genetic Background | Generation |
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Allele Type |
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Transgenic (Conditional ready (e.g. floxed), Reporter, Constitutively active, Inserted expressed sequence, Humanized sequence) |
caBmpr1a transgenic mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice possess a floxed Tau-β-galactosidase gene followed by a constitutively active mutant form of human BMP receptor type IA (caBmpr1a Q227D), an IRES sequence, and the EGFP coding sequence. Transgenic mice have widespread expression of lacZ prior to exposure to Cre recombinase. When bred with mice that express Cre recombinase, offspring will have the floxed-lacZ gene deleted and subsequent expression of caBmpr1a and EGFP in the cre-expressing cells. These mice may be useful for studying BMP signaling in tissues where Cre expresses such as cartilage and muscle, osteoclastogenesis (via RANKL-OPG pathway and/or canonical Wnt signaling), age dependant bone loss, osteoporosis, chondrodysplasia, fibrodysplasia ossificans progressive, and mineralization in muscle/skeletal development.
The transgene was designed with (from 5' to 3') the CMV-IE enhancer/chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector), a loxP-flanked Tau-β-galactosidase (lacZ) cassette and 3x-polyA signal, a C-terminal hemagglutinin (HA)-tagged human ALK3 (BMP receptor type IA (BMPR1A)) gene modified to be constitutively active by missense mutation (Q233D) in a glycine-serine (GS) rich domain (caBmpr1a), an internal ribosome entry site (IRES), and an enhanced green fluorescent protein (EGFP) sequence followed by a polyA signal. The donating investigator reports that this transgene was injected into 129S7/SvEvBrd-Hprt1b-m2 embryonic stem (ES) cells to generate the founder line, and transgenic mice on a mixed C57BL/6J;129SvEv genetic background were sent to the MMRRC at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J inbred mice for at least one generation to establish the colony.
Expressed Gene | BMPR1A, bone morphogenetic protein receptor type 1A, human |
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Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | Widespread expression of lacZ. When bred with mice that express Cre recombinase, caBmpr1a and EGFP will be expressed in the cre-expressing cells. |
Allele Name | transgene insertion 1, Yuji Mishina |
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Allele Type | Transgenic (Conditional ready (e.g. floxed), Reporter, Constitutively active, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | caBmpr1a |
Gene Symbol and Name | Tg(CAG-lacZ,-BMPR1A*,-EGFP)1Mis, transgene insertion 1, Yuji Mishina |
Gene Synonym(s) | |
Promoter | ACTB, actin, beta, chicken |
Expressed Gene | BMPR1A, bone morphogenetic protein receptor type 1A, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | Widespread expression of lacZ. When bred with mice that express Cre recombinase, caBmpr1a and EGFP will be expressed in the cre-expressing cells. |
Strain of Origin | 129S7/SvEvBrd-Hprtb-m2 |
Chromosome | UN |
Molecular Note | The modified chicken beta-actin promoter (CAG) was used to drive ubiquitous expression of a floxed lacZ gene with a nuclear localization signal and a triple polyadenylation sequence. The floxed cassette is followed by a constitutively active human BMPR1A sequence with the amino acid substitution of aspartic acid for glutamine at position 227 (Q227D), an IRES sequence, and the EGFP coding sequence. Expression of the constitutively active BMPR1A and EGFP are cre-dependent. No line information is available. |
Mutations Made By | Manas Ray, NIEHS |
When maintaining a live colony, hemizygous mice may be bred to wildtype (noncarrier) mice from the colony or to C57BL/6J inbred mice.
When using the caBmpr1a mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32018 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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