This targeted mutation of the myelocytomatosis oncogene (Myc) gene may be useful in generating conditional mutations to study B cell activation and proliferation, neural crest cell development, and mouse embryonic fibroblast proliferation.
Ignacio Moreno de Alboran, Universidad de Autonoma de Madrid
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Myc | myelocytomatosis oncogene |
Homozygote: Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene.
Heterozygote: Not evaluated
For example, when bred to mice carrying Cd19tm1(cre)Cgn, which expresses Cre recombinase in B lymphoctye development, this mutant mouse strain may be useful in studies of B cell proliferation and activation.
When bred to mice carrying Tg(Wnt1-cre)11Rth, which expresses Cre recombinase in the neural tube, this mutant mouse strain may be useful in studies of craniofacial development.
A targeting vector was designed to place a loxP site between exons 1 and 2 and a loxP-flanked PGKneo cassette downstream of exon 3. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The neomycin cassette was removed by transient transfection with a Cre recombinase expressing plasmid leaving loxP sites flanking exons 2 and 3. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then bred to mice on a mixed B6;129 background carrying the Mycntm1Psk allele. The Mycn allele was subsequently bred out of the line. The mice were then backcrossed to C57BL/6J for 6 generations before being transferred to the Repository. The mice were crossed to C57BL/6J to establish the colony.
Allele Name | targeted mutation 2, Frederick W Alt |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | c-mycfl; MYCfl; Mycflox; Myclox |
Gene Symbol and Name | Myc, myelocytomatosis oncogene |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 15 |
Molecular Note | A single loxP site was inserted into intron 1 and a loxP-flanked neomycin selection cassette was inserted 3' to exon 3. The neomycin cassette was removed in ES cells by transient Cre expression prior to the production of chimeric mice. |
Mutations Made By | Dr. Frederick Alt, Children's Hospital |
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6.129S6-Myctm2Fwa/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32046 in your Materials and Methods section.
Facility Barrier Level Descriptions
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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