This targeted mutation of the myelocytomatosis oncogene (Myc) gene may be useful in generating conditional mutations to study B cell activation and proliferation, neural crest cell development, and mouse embryonic fibroblast proliferation.
Ignacio Moreno de Alboran, Universidad de Autonoma de Madrid
Homozygote: Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene.
Heterozygote: Not evaluated
For example, when bred to mice carrying Cd19tm1(cre)Cgn, which expresses Cre recombinase in B lymphoctye development, this mutant mouse strain may be useful in studies of B cell proliferation and activation.
When bred to mice carrying Tg(Wnt1-cre)11Rth, which expresses Cre recombinase in the neural tube, this mutant mouse strain may be useful in studies of craniofacial development.
A targeting vector was designed to place a loxP site between exons 1 and 2 and a loxP-flanked PGKneo cassette downstream of exon 3. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The neomycin cassette was removed by transient transfection with a Cre recombinase expressing plasmid leaving loxP sites flanking exons 2 and 3. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then bred to mice on a mixed B6;129 background carrying the Mycntm1Psk allele. The Mycn allele was subsequently bred out of the line. The mice were then backcrossed to C57BL/6J for 6 generations before being transferred to the Repository. The mice were crossed to C57BL/6J to establish the colony.
|Allele Name||targeted mutation 2, Frederick W Alt|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Allele Synonym(s)||c-mycfl; MYCfl; Mycflox; Myclox|
|Gene Symbol and Name||Myc, myelocytomatosis oncogene|
|Strain of Origin||129S6/SvEvTac|
|Molecular Note||A single loxP site was inserted into intron 1 and a loxP-flanked neomycin selection cassette was inserted 3' to exon 3. The neomycin cassette was removed in ES cells by transient Cre expression prior to the production of chimeric mice.|
|Mutations Made By|| |
Dr. Frederick Alt, Children's Hospital
While maintaining a live colony, these mice are bred as homozygotes.
When using the B6.129S6-Myctm2Fwa/Mmjax mouse strain in a publication, please cite the originating article(s) and include MMRRC stock #32046 in your Materials and Methods section.