B6.129S6-Myc^tm2Fwa/Mmjax

Stock number: 011123
Research areas: Developmental Biology Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

This targeted mutation of the myelocytomatosis oncogene (Myc) gene may be useful in generating conditional mutations to study B cell activation and proliferation, neural crest cell development, and mouse embryonic fibroblast proliferation.

Detailed description

Homozygote: Mice homozygous for the targeted mutation are viable and fertile and exhibit no overt phenotypic abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific conditional mutants of the floxed gene.

Heterozygote: Not evaluated

For example, when bred to mice carrying Cd19^tm1(cre)Cgn, which expresses Cre recombinase in B lymphoctye development, this mutant mouse strain may be useful in studies of B cell proliferation and activation.

When bred to mice carrying Tg(Wnt1-cre)11Rth, which expresses Cre recombinase in the neural tube, this mutant mouse strain may be useful in studies of craniofacial development.

Development

A targeting vector was designed to place a loxP site between exons 1 and 2 and a loxP-flanked PGKneo cassette downstream of exon 3. The construct was electroporated into 129S6/SvEvTac-derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The neomycin cassette was removed by transient transfection with a Cre recombinase expressing plasmid leaving loxP sites flanking exons 2 and 3. The resulting chimeric animals were crossed to C57BL/6 mice. The mice were then bred to mice on a mixed B6;129 background carrying the Mycn^tm1Psk allele. The Mycn allele was subsequently bred out of the line. The mice were then backcrossed to C57BL/6J for 6 generations before being transferred to the Repository. The mice were crossed to C57BL/6J to establish the colony.

Allele

Symbol: Myc^tm2Fwa
Name: targeted mutation 2, Frederick W Alt
MGI accession ID: MGI:2178233
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Myc
Marker name: myelocytomatosis oncogene
Chromosome: 15
Strain of origin: 129S6/SvEvTac
Molecular note: A single loxP site was inserted into intron 1 and a loxP-flanked neomycin selection cassette was inserted 3' to exon 3. The neomycin cassette was removed in ES cells by transient Cre expression prior to the production of chimeric mice.