These transgenic mice express Cre recombinase under the control of the mouse growth differentiation factor 9 (Gdf9) promoter. Cre recombinase expression is detected in oocytes of the primordial follicles by postnatal day 3. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in studies of folliculogenesis and oocyte development.
Austin Cooney, Baylor College of Medicine
Genetic Background | Generation |
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Allele Type |
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Transgenic (Recombinase-expressing) |
Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse growth differentiation factor 9 (Gdf9) promoter. Cre recombinase expression is detected in oocytes of the primordial follicles by postnatal day 3 and in oocytes, but not somatic cells, of all follicles at the primary, secondary and later stages by 24 days. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in the offspring. This mutant mouse strain may be useful in studies of studies of folliculogenesis and oocyte development.
A transgenic construct containing sequence encoding improved Cre recombinase (iCre) under the control of the mouse growth differentiation factor 9 (Gdf9) promoter was introduced into C57BL/6 fertilized mouse eggs. Founder line 5092 was subsequently established and crossed to a C57BL/6 male by the donating laboratory. In addition, the strain was crossed to ICR outbred mice. Upon arrival, mice were bred to C57BL/6J to establish the colony.
A 32 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 9 of 32 markers (chromosomes 1, 2, 6, 8, 11, 13, 15, 16 and 19) that were not C57BL/6 allele-type. These markers are 129 allele-type and suggest the strain was outcrossed prior to arrival at The Jackson Laboratory.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | Cre recombinase expression is detected in oocytes of the primordial follicles by postnatal day 3 and in oocytes. |
Allele Name | transgene insertion 5092, Austin J Cooney |
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Allele Type | Transgenic (Recombinase-expressing) |
Allele Synonym(s) | GDF-9-Cre; GDF-9-iCre; Tg(Gdf9-cre)5092Coo |
Gene Symbol and Name | Tg(Gdf9-icre)5092Coo, transgene insertion 5092, Austin J Cooney |
Gene Synonym(s) | |
Promoter | Gdf9, growth differentiation factor 9, mouse, laboratory |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | Cre recombinase expression is detected in oocytes of the primordial follicles by postnatal day 3 and in oocytes. |
Strain of Origin | C57BL/6 |
Chromosome | UN |
Molecular Note | The transgene is composed of the Gdf9 promoter sequence, an improved Cre (iCre) expression cassette, and SV40 pA. The promoter is active in the oocytes in lines 5092 and 5090. |
Mutations Made By | Austin Cooney, Baylor College of Medicine |
While maintaining a live colony, these mice are bred as hemizygotes. The donating investigator indicates that homozygotes are viable, but did not maintain this line as a homozygote x homozygote mating.
When using the GDF9-iCRE mouse strain in a publication, please cite the originating article(s) and include JAX stock #011062 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non-carrier for Tg(Gdf9-cre)5092Coo |
Frozen Mouse Embryo | STOCK Tg(Gdf9-icre)5092Coo/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Gdf9-icre)5092Coo/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK Tg(Gdf9-icre)5092Coo/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK Tg(Gdf9-icre)5092Coo/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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