STOCK Tg(Gdf9-icre)5092Coo/J

Stock number: 011062
Product name: GDF9-iCRE
Research areas: Research Tools

Description

These transgenic mice express Cre recombinase under the control of the mouse growth differentiation factor 9 (Gdf9) promoter. Cre recombinase expression is detected in oocytes of the primordial follicles by postnatal day 3. This strain represents an effective tool for generating tissue specific-targeted mutants that would be useful in studies of folliculogenesis and oocyte development.

Detailed description

Hemizygous mice are viable, fertile, normal in size, and do not display any gross physical or behavioral abnormalities. These transgenic mice express Cre recombinase under the control of the mouse growth differentiation factor 9 (Gdf9) promoter. Cre recombinase expression is detected in oocytes of the primordial follicles by postnatal day 3 and in oocytes, but not somatic cells, of all follicles at the primary, secondary and later stages by 24 days. When crossed with a strain containing loxP site flanked sequence, Cre-mediated recombination results in deletion of the flanked sequence in the offspring. This mutant mouse strain may be useful in studies of studies of folliculogenesis and oocyte development.

Development

A transgenic construct containing sequence encoding improved Cre recombinase (iCre) under the control of the mouse growth differentiation factor 9 (Gdf9) promoter was introduced into C57BL/6 fertilized mouse eggs. Founder line 5092 was subsequently established and crossed to a C57BL/6 male by the donating laboratory. In addition, the strain was crossed to ICR outbred mice. Upon arrival, mice were bred to C57BL/6J to establish the colony.

A 32 SNP (single nucleotide polymorphism) panel analysis performed by The Jackson Laboratory revealed 9 of 32 markers (chromosomes 1, 2, 6, 8, 11, 13, 15, 16 and 19) that were not C57BL/6 allele-type. These markers are 129 allele-type and suggest the strain was outcrossed prior to arrival at The Jackson Laboratory.

Allele

Symbol: Tg(Gdf9-icre)5092Coo
Name: transgene insertion 5092, Austin J Cooney
MGI accession ID: MGI:3056522
Generation method: Transgenic
Attributes: Recombinase-expressing
Marker symbol: Tg(Gdf9-icre)5092Coo
Marker name: transgene insertion 5092, Austin J Cooney
Chromosome: UN
Strain of origin: C57BL/6
Expressed genes: cre,cre recombinase,bacteriophage P1
Site of expression: Cre recombinase expression is detected in oocytes of the primordial follicles by postnatal day 3 and in oocytes.
Molecular note: The transgene is composed of the Gdf9 promoter sequence, an improved Cre (iCre) expression cassette, and SV40 pA. The promoter is active in the oocytes in lines 5092 and 5090.