Cst-T2A-Cre (Cort-T2A-Cre) mice have Cre recombinase expression directed to Cort-expressing cells (CST positive neurons) by the endogenous promoter/enhancer elements of the cortistatin locus.
Z. Josh Huang, Cold Spring Harbor Laboratory
Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Recombinase-expressing) | Cort | cortistatin |
The Cst-T2A-Cre allele (also called Cort-T2A-Cre or Cst-2A-Cre) harbors a a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus) and Cre recombinase in the 3' UTR of the cortistatin locus (Cort). As such, cre expression is directed by the endogenous Cort promoter/enhancer elements. When Cst-T2A-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cort-expressing cells (CST positive neurons) of the offspring. Cort expression from the Cst-T2A-Cre allele has not been evaluated. Additional phenotype information described below.
In 2010, the donating investigator reported Cre recombinase activity is specific and efficient (largely recapitulates the endogenous Cort expression pattern with highly efficient recombination). They report Cre recombinase activity is observed in some GABAergic interneurons, and did not examine cre expression in the intestine or tissues other than brain. Cort expression from the Cst-T2A-Cre allele was not evaluated. They also reported that homozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.
For characterization information, see images at the Allen Institute for Brain Science website (Cort-T2A-Cre images).
If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Corttm1(cre)Zjh). This same information would also be found searching the MGI Recombinase Activity database.
A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cortistatin locus (Cort). This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10); see Stock No. 005703) to generate the colony and remove the neo selection cassette. These Cst-T2A-Cre mice (also called Cort-T2A-Cre or Cst-2A-Cre) were subsequently bred together for several generations (and the FLPe transgene was removed) prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
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Site of Expression | When Cst-T2A-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cort-expressing cells (CST positive neurons) of the offspring. |
Allele Name | targeted mutation 1, Z Josh Huang |
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Allele Type | Targeted (Recombinase-expressing) |
Allele Synonym(s) | Corttm1(cre)Zjh; targeted mutation 1, Z Josh Huang |
Gene Symbol and Name | Cort, cortistatin |
Gene Synonym(s) | CST-14; CST-17; CST-29; CST; PCST |
Expressed Gene | cre, cre recombinase, bacteriophage P1 |
Site of Expression | When Cst-T2A-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cort-expressing cells (CST positive neurons) of the offspring. |
Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Chromosome | 4 |
Molecular Note | A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), a cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cortistatin locus (Cort). This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to generate the colony and remove the neo selection cassette. The FLPe transgene has been bred out of the line. |
Mutations Made By | Z. Josh Huang, Cold Spring Harbor Laboratory |
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Corttm1(cre)Zjh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010910 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service | Genotype | Price |
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Heterozygous for Cort<tm1(cre)Zjh> |
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