Overview

Cst-T2A-Cre (Cort-T2A-Cre) mice have Cre recombinase expression directed to Cort-expressing cells (CST positive neurons) by the endogenous promoter/enhancer elements of the cortistatin locus.

Donating Investigator

Z. Josh Huang, Cold Spring Harbor Laboratory

Genetic overview

Genetic Background	Generation

Cort^tm1(cre)Zjh

Allele Type	Gene Symbol	Gene Name
Targeted (Recombinase-expressing)	Cort	cortistatin

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Research Applications

Research Tools
Neurobiology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

The Cst-T2A-Cre allele (also called Cort-T2A-Cre or Cst-2A-Cre) harbors a a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus) and Cre recombinase in the 3' UTR of the cortistatin locus (Cort). As such, cre expression is directed by the endogenous Cort promoter/enhancer elements. When Cst-T2A-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cort-expressing cells (CST positive neurons) of the offspring. Cort expression from the Cst-T2A-Cre allele has not been evaluated. Additional phenotype information described below.

In 2010, the donating investigator reported Cre recombinase activity is specific and efficient (largely recapitulates the endogenous Cort expression pattern with highly efficient recombination). They report Cre recombinase activity is observed in some GABAergic interneurons, and did not examine cre expression in the intestine or tissues other than brain. Cort expression from the Cst-T2A-Cre allele was not evaluated. They also reported that homozygous mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities.

For characterization information, see images at the Allen Institute for Brain Science website (Cort-T2A-Cre images).

If the recombinase activity pattern of this allele is further characterized by the Genetic Resource Science group at The Jackson Laboratory, such findings will be reported on the Mouse Genome Informatics (MGI) Allele Detail entry (Cort^tm1(cre)Zjh). This same information would also be found searching the MGI Recombinase Activity database.

Development

A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), a Cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cortistatin locus (Cort). This construct was electroporated into (C57BL/6 x 129S4Sv/Jae)-derived V6.5 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background (N10); see Stock No. 005703) to generate the colony and remove the neo selection cassette. These Cst-T2A-Cre mice (also called Cort-T2A-Cre or Cst-2A-Cre) were subsequently bred together for several generations (and the FLPe transgene was removed) prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J inbred mice (Stock No. 000664) for at least one generation to establish the colony.

Expression Data

Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	When Cst-T2A-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cort-expressing cells (CST positive neurons) of the offspring.

Control Suggestions

Additional Information

Considerations for Choosing Controls

Selected References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data SubmissionMGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

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Genetics

Cort^tm1(cre)Zjh

Allele Symbol: Cort^tm1(cre)Zjh

Allele Name	targeted mutation 1, Z Josh Huang
Allele Type	Targeted (Recombinase-expressing)
Allele Synonym(s)	Cort^tm1(cre)Zjh; targeted mutation 1, Z Josh Huang
Gene Symbol and Name	Cort, cortistatin
Gene Synonym(s)	CST-14; CST-17; CST-29; CST; PCST
Expressed Gene	cre, cre recombinase, bacteriophage P1
Site of Expression	When Cst-T2A-Cre mice are bred with mice containing loxP-flanked sequences, Cre-mediated recombination will result in deletion of the floxed sequences in the Cort-expressing cells (CST positive neurons) of the offspring.
Strain of Origin	(C57BL/6 x 129S4/SvJae)F1
Chromosome	4
Molecular Note	A targeting vector was designed to insert a T2A oligopeptide that mediates ribosomal skipping (foot-and-mouth disease virus 2A from the insect Thosea asigna virus), a cre recombinase sequence, an SV40 polyA signal, and an frt-flanked neo cassette into the 3' untranslated region (after the translational termination site) of the cortistatin locus (Cort). This construct was electroporated into C57BL6/S129 hybrid embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient tetraploid blastocysts. Chimeric mice were bred with Actin-FLPe mice (on a C57BL/6 congenic background) to generate the colony and remove the neo selection cassette. The FLPe transgene has been bred out of the line.
Mutations Made By	Z. Josh Huang, Cold Spring Harbor Laboratory

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Research Tools
- Genetics Research
  - Mutagenesis and Transgenesis
  - Tissue/Cell Markers
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: neurons
  - Tissue/Cell Markers: Cre-lox System
- Neurobiology Research
  - cell marker
- Cre-lox System
  - Cre Recombinase Expression
Neurobiology Research
- Cre-lox System
  - Cre Recombinase expression in neural tissue

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Cort^tm1(cre)Zjh/Cort^tm1(cre)Zjh
involves: 129S4/SvJae * C57BL/6 * SJL

normal phenotype

no abnormal phenotype detected
- Normal - mice are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities

References

NIH Neuroscience Blueprint Cre Driver Network. 2009. Cre recombinase-expressing mice generated for the NIH Neuroscience Blueprint Cre Driver Network MGI Direct Data SubmissionMGI: J:151755
Taniguchi H; He M; Wu P; Kim S; Paik R; Sugino K; Kvitsani D; Fu Y; Lu J; Lin Y; Miyoshi G; Shima Y; Fishell G; Nelson SB; Huang ZJ. 2011. A resource of cre driver lines for genetic targeting of GABAergic neurons in cerebral cortex. Neuron 71(6):995-1013PubMed: 21943598 MGI: J:177261

Additional - Cort^tm1(cre)Zjh related

Technical Support

Contact Technical Support

Genotyping Protocols
- Probe: Gt(rosa)26sor^Tm1Sor Probe
- Melt Curve Analysis: Cort^tm1(cre)Zjh
- Standard PCR: Cort^tm1(cre)Zjh
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK Cort^tm1(cre)Zjh/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010910 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
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Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for Cort<tm1(cre)Zjh>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Corttm1(cre)Zjh

Allele Symbol: Corttm1(cre)Zjh

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Corttm1(cre)Zjh/Corttm1(cre)Zjhinvolves: 129S4/SvJae * C57BL/6 * SJL

normal phenotype

References

Additional - Corttm1(cre)Zjh related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Cort^tm1(cre)Zjh

Allele Symbol: Cort^tm1(cre)Zjh

Genotype: Cort^tm1(cre)Zjh/Cort^tm1(cre)Zjh
involves: 129S4/SvJae * C57BL/6 * SJL

Additional - Cort^tm1(cre)Zjh related