Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are viable but sterile. No gene product (mRNA or protein) is detected by RT-PCR analysis of testes from homozygotes. Male homozygotes display decreased testis weight and incomplete spermatogenesis (arrest at the pachytene stage). Female homozygotes exhibit reduced germ cell number in neonatal ovaries and no forming follicles. Homozygotes have abnormal homologous chromosome pairing, impaired double strand break repair and defective sex body (XY body) formation. This mutant mouse strain may be useful in studies of meiotic arrest, infertility, and double-stranded break repair.
A targeting vector containing the lacZ gene and a loxP-flanked PGKneo cassette was used to disrupt part of exon 2, which contains the initiation codon, all of exons 3 to 6, and part of exon 7. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for an unknown number of generations.
|Allele Name||targeted mutation 1, Yasuhisa Matsui|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Prdm9, PR domain containing 9|
|Strain of Origin||129P2/OlaHsd|
|Molecular Note||A targeting vector containing the lacZ gene and a loxP-flanked PGKneo cassette disrupted part of exon 2, which contains the initiation codon, all of exons 3 to 6, and part of exon 7. RT-PCR analysis of testes from homozygous mutant mice did not detect transcript.|
|Mutations Made By|| |
Yasuhisa Matsui, Tohoku University
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are viable but sterile.
When using the Meisetz- mouse strain in a publication, please cite the originating article(s) and include JAX stock #010719 in your Materials and Methods section.