Mice homozygous for this knockout allele exhibit germ cell defects resulting in sterility.
Yasuhisa Matsui, Tohoku University
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Prdm9 | PR domain containing 9 |
Mice that are heterozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. Homozygotes are viable but sterile. No gene product (mRNA or protein) is detected by RT-PCR analysis of testes from homozygotes. Male homozygotes display decreased testis weight and incomplete spermatogenesis (arrest at the pachytene stage). Female homozygotes exhibit reduced germ cell number in neonatal ovaries and no forming follicles. Homozygotes have abnormal homologous chromosome pairing, impaired double strand break repair and defective sex body (XY body) formation. This mutant mouse strain may be useful in studies of meiotic arrest, infertility, and double-stranded break repair.
A targeting vector containing the lacZ gene and a loxP-flanked PGKneo cassette was used to disrupt part of exon 2, which contains the initiation codon, all of exons 3 to 6, and part of exon 7. The construct was electroporated into 129P2/OlaHsd derived E14 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to C57BL/6 mice for an unknown number of generations.
Allele Name | targeted mutation 1, Yasuhisa Matsui |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Meisetz- |
Gene Symbol and Name | Prdm9, PR domain containing 9 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 17 |
Molecular Note | A targeting vector containing the lacZ gene and a loxP-flanked PGKneo cassette disrupted part of exon 2, which contains the initiation codon, all of exons 3 to 6, and part of exon 7. RT-PCR analysis of testes from homozygous mutant mice did not detect transcript. |
Mutations Made By | Yasuhisa Matsui, Tohoku University |
When maintaining a live colony, these mice can be bred as heterozygotes. Homozygotes are viable but sterile.
When using the Meisetz- mouse strain in a publication, please cite the originating article(s) and include JAX stock #010719 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Prdm9<tm1Ymat> |
Frozen Mouse Embryo | B6;129P2-Prdm9<tm1Ymat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Prdm9<tm1Ymat>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Prdm9<tm1Ymat>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Prdm9<tm1Ymat>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.