These mutant mice may be useful in generating conditional mutations for studies of embryogenesis and early development.
Thomas Gridley, Maine Medical Center Research Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Snai1 | snail family zinc finger 1 |
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 and 3 deleted in the cre-expressing tissue(s).
A targeting vector containing a FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 1 of the targeted gene, and a loxP site was inserted upstream of the the FRT site flanked neo cassette. A second loxP site was inserted between exons 2 and 3. This construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (STOCK no. 3946) to remove the selection cassette. Mice that retained the loxP site flanked exons 1 and 2 were then bred to C57BL/6J mice to remove the Flp recombinase allele. These mice no longer carry the Gt(ROSA)26Sortm1(FLP1)Dym allele. The mice were then crossed to C57BL/6J once to establish the colony.
Allele Name | targeted mutation 1, Tom Gridley |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Snai1flox |
Gene Symbol and Name | Snai1, snail family zinc finger 1 |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 2 |
Molecular Note | LoxP sites were inserted to flank promoter sequences, exons 1 and 2. The frt flanked neo cassette was removed by flp mediated recombination. Deletion of this sequence would remove the start codon, the 5' SNAG repression domain and the first three zinc finger DNA binding domains of the protein. |
Mutations Made By | Thomas Gridley, Maine Medical Center Research Institute |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129S-Snai1tm2Grid/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010686 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Snai1<tm2Grid> |
Frozen Mouse Embryo | B6;129S-Snai1<tm2Grid>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S-Snai1<tm2Grid>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129S-Snai1<tm2Grid>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129S-Snai1<tm2Grid>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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