These mutant mice may be useful in generating conditional mutations for studies of embryogenesis and early development.
Thomas Gridley, Maine Medical Center Research Institute
These mice possess loxP sites on either side of exons 2 and 3 of the targeted gene. Mice that are homozygous for this allele are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When these mutant mice are bred to mice that express Cre recombinase, resulting offspring will have exon 2 and 3 deleted in the cre-expressing tissue(s).
A targeting vector containing a FRT site flanked PGK-Neo selection cassette was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 1 of the targeted gene, and a loxP site was inserted upstream of the the FRT site flanked neo cassette. A second loxP site was inserted between exons 2 and 3. This construct was electroporated into 129S1/Sv-Oca2+ Tyr+ Kitl+ derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to 129S4/SvJaeSor-Gt(ROSA)26Sortm1(FLP1)Dym/J (STOCK no. 3946) to remove the selection cassette. Mice that retained the loxP site flanked exons 1 and 2 were then bred to C57BL/6J mice to remove the Flp recombinase allele. These mice no longer carry the Gt(ROSA)26Sortm1(FLP1)Dym allele. The mice were then crossed to C57BL/6J once to establish the colony.
|Allele Name||targeted mutation 1, Tom Gridley|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Snai1, snail family zinc finger 1|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||LoxP sites were inserted to flank promoter sequences, exons 1 and 2. The frt flanked neo cassette was removed by flp mediated recombination. Deletion of this sequence would remove the start codon, the 5' SNAG repression domain and the first three zinc finger DNA binding domains of the protein.|
|Mutations Made By|| |
Thomas Gridley, Maine Medical Center Research Institute
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129S-Snai1tm2Grid/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010686 in your Materials and Methods section.
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