This transgenic line expresses the human ERBB2 (HER2) gene in mammary gland and brain, displays tolerance to ERBB2 antigen and may be useful in testing ERBB2-based vaccines and immunotherapies.
Wei-Zen Wei, Wayne State University
Genetic Background | Generation |
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Allele Type |
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Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
Mice that are hemizygous for the transgene are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. These transgenic mice express human ERBB2 (HER2) under the control of the mouse whey acid protein promoter (Wap). The Wap promoter directs expression to the mammary gland and molecular layer of the cerebellum. Mice do not develop spontaneous tumors, but are tolerant to ERBB2 tumor associated antigen, developing tumors after inoculation with ERBB2 expressing tumor cells. Anti-tumor immunity can be induced using aggressive DNA vaccination. This mutant mouse strain may be useful in testing ERBB2-based vaccines and immunotherapies.
Sequencing has identified the transgene integration site as intron 2 of the Pds5b gene (PDS5 cohesin associated factor B) on chromosome 5. The 162 copy (approx 1.1 Mb) integration disrupts Pds5b rendering it a null allele. Embryos homozygous for the mutation are severely underdeveloped with jaundice and disruptions to limb and facial formation by E14-15, no embryos survive to birth.
A transgenic construct containing a mouse 2.6 kb whey acidic protein (Wap) promoter fused to a human ERBB2 4.4 kb cDNA followed by the bovine growth hormone polyadenylation signal. Transgenic mice were generated by microinjection into (C57BL/6 x C3H) F1 mouse embryos. This line has been backcrossed to C57BL/6 for more than 25 generations by the donating investigator.
As reported by Yong CSM et al (PLoS One 2015; 10(9):e0136817), the transgene inserted as a 162 bp concatemer (approx. 1.1 Mb) 19 nucleotides upstream of exon 3 of the Pds5b gene on Chr 5. The insertion disrupts Pds5b resulting in a null allele.
Expressed Gene | ERBB2, erb-b2 receptor tyrosine kinase 2, human |
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Site of Expression |
Allele Name | transgene insertion 229, Wei-Zen Wei |
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Allele Type | Transgenic (Null/Knockout, Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | Her-2 Tg; Tg(Wap-ERBB2)229Wzw; WAP-Her-2 |
Gene Symbol and Name | Pds5b, PDS5 cohesin associated factor B |
Gene Synonym(s) | |
Promoter | Wap, whey acidic protein, mouse, laboratory |
Expressed Gene | ERBB2, erb-b2 receptor tyrosine kinase 2, human |
Strain of Origin | (C57BL/6 x C3H)F1 |
Chromosome | 5 |
Molecular Note | A transgenic construct containing a mouse 2.6 kb whey acidic protein (Wap) promoter fused to a human ERBB2 4.4 kb cDNA followed by the bovine growth hormone polyadenylation signal. Line 229 inserted into intron 2 (19 bp upstream of exon 3). |
Mutations Made By | Wei-Zen Wei, Wayne State University |
While maintaining a live colony, these mice are bred as hemizygotes. Homozygotes are embryonic lethal.
When using the B6.Cg-Pds5bTg(Wap-ERBB2)229Wzw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010562 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or non-carrier for <Tg(Wap-ERBB2)229Wzw> |
Frozen Mouse Embryo | B6.Cg-Pds5b<Tg(Wap-ERBB2)229Wzw>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Pds5b<Tg(Wap-ERBB2)229Wzw>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Pds5b<Tg(Wap-ERBB2)229Wzw>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Pds5b<Tg(Wap-ERBB2)229Wzw>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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