These catalytic subunit of DNA polymerase zeta (Rev3l) floxed mice may be useful in generating conditional mutations to study genome instability, DNA repair and class switch recombination.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Rev3l | REV3 like, DNA directed polymerase zeta catalytic subunit |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. When used in conjunction with a Cre recombinase-expressing strain, this strain is useful in generating tissue-specific mutants of the floxed allele. Excision of the floxed fragment in B cells results in chromosomal instability, impaired B cell proliferation, reduced class switch recombination, reduced frequency of somatic mutations and increased frequency of DNA breaks. This mutant mouse strain is useful in studies of genome instability, DNA break repair and class switch recombination.
For example, when crossed to a strain expressing Cre recombinase in Mature B cells (see Stock No. 006368), this mutant mouse strain may be useful in studies of class switch recombination and DNA break repair.
A loxP flanked targeting vector containing neomycin resistance genes was utilized in the construction of this mutant. This selection cassette was inserted upstream of exon 2 of the targeted gene, and another loxP site was inserted downstream of exon 2. The construct was electroporated into C57BL/6 derived Bruce 4 embryonic stem (ES) cells. Transient Cre expression in targeted cells excised the neo cassette, but left the loxP-flanked exon 2. Correctly targeted ES cells were injected into blastocysts. The donating investigator reported that resulting chimeric animals were crossed to C57BL/6.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, at least 1 of 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a mixed C57BL/6J ; C57BL/6N genetic background.
Allele Name | targeted mutation 1, Klaus Rajewsky |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Pol-zetaf |
Gene Symbol and Name | Rev3l, REV3 like, DNA directed polymerase zeta catalytic subunit |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 10 |
Molecular Note | Exon 2 was targeted conditional deletion becuase messenger RNA splicing from exon 1 to exon 3 would result in a frameshift mutation. A floxed neomycin resistance cassette was placed upstream of the exon, and an additional loxP site was inserted downstream of the exon. After targeting, the neomycin cassette was removed by transient expression of Cre recombinase. Genomic PCR confirmed correct targeting of the locus. |
Mutations Made By | Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine |
While maintaining a live colony, these mice are bred as homozygotes.
When using the C57BL/6-Rev3ltm1Rsky/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #010540 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Rev3l<tm1Rsky> |
Frozen Mouse Embryo | C57BL/6-Rev3l<tm1Rsky>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Rev3l<tm1Rsky>/J | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Rev3l<tm1Rsky>/J | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Rev3l<tm1Rsky>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.