These TANK1 knockout mice have exon 1 of the Tnks gene deleted. Homozygous (TANK1-/-) mice may be useful for studying the post-translational modification of proteins. These TANK1-mutant mice may be used in conjunction with TANK2-mutant mice (Stock No. 006200).
Richard J Hodes, NCI/NIH
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tnks | tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase |
Homozygous (TANK1-/-) mice are viable and fertile with no reported developmental or gross physical abnormalities. As exon 1 is deleted from the targeted allele, no full length protein (TANK1) is detected in thymus, testis or spleen tissues. Because the deletion does not encompass the potential alternative promoter between exons 4-5, an alternative transcript and subsequent low molecular weight protein (TANK1a) is expressed in testis (but not in other assayed tissues). These TANK1-mutant mice may be useful for studying the post-translational modification of proteins associated with cell cycle, mitosis, telomere length maintenance/aging, DNA replication/repair, cellular senescence, apoptosis, tumorigenesis, vesicle trafficking, and insulin responses. These TANK1-mutant mice may also be used along with TANK2-mutant mice (Stock No. 006200).
A targeting vector was designed to insert a loxP site upstream of exon 1, and a loxP-flanked neo cassette (in reverse orientation) just downstream of exon 1 of the targeted gene. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 females to establish the colony. The TANK1flox Mice were then bred with EIIa-cre mice (C57BL/6 congenic background; similar to Stock No. 003724). The resulting mice lacking both exon 1 and the floxed neo cassette (TANK1-) were selectively bred to remove the EIIa-cre transgene. The donating investigator reports that heterozygous mice were backcrossed to C57BL/6NCr for at least 10 generations, and then bred together to generate homozygotes. Homozygous embryos were sent to The Jackson Laboratory Repository.
Allele Name | targeted mutation 1.1, Y Jeffrey Chiang |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | TANK1- |
Gene Symbol and Name | Tnks, tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase |
Gene Synonym(s) | |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | 8 |
Molecular Note | Exon 1 was removed by germ line, cre-mediated recombination. The absence of protein expression was confirmed by western blot analysis on testis, kidney and spleen extracts. |
Mutations Made By | Richard Hodes, NCI/NIH |
When maintaining a live colony, homozygous mice may be bred together.
When using the TANK1 knock-out (TANK1-) mouse strain in a publication, please cite the originating article(s) and include JAX stock #009685 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Tnks<tm1.1Yjc> |
Frozen Mouse Embryo | B6N.129S1(Cg)-Tnks<tm1.1Yjc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S1(Cg)-Tnks<tm1.1Yjc>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6N.129S1(Cg)-Tnks<tm1.1Yjc>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6N.129S1(Cg)-Tnks<tm1.1Yjc>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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