B6N.129S1(Cg)-Tnks^tm1.1Yjc/J

Stock number: 009685
Product name: TANK1 knock-out (TANK1-)
Research areas: Apoptosis Research, Cancer Research, Cell Biology Research, Developmental Biology Research, Diabetes and Obesity Research, Research Tools

Description

These TANK1 knockout mice have exon 1 of the Tnks gene deleted. Homozygous (TANK1^-/-) mice may be useful for studying the post-translational modification of proteins. These TANK1-mutant mice may be used in conjunction with TANK2-mutant mice (Stock No. 006200).

Detailed description

Homozygous (TANK1^-/-) mice are viable and fertile with no reported developmental or gross physical abnormalities. As exon 1 is deleted from the targeted allele, no full length protein (TANK1) is detected in thymus, testis or spleen tissues. Because the deletion does not encompass the potential alternative promoter between exons 4-5, an alternative transcript and subsequent low molecular weight protein (TANK1a) is expressed in testis (but not in other assayed tissues). These TANK1-mutant mice may be useful for studying the post-translational modification of proteins associated with cell cycle, mitosis, telomere length maintenance/aging, DNA replication/repair, cellular senescence, apoptosis, tumorigenesis, vesicle trafficking, and insulin responses. These TANK1-mutant mice may also be used along with TANK2-mutant mice (Stock No. 006200).

Development

A targeting vector was designed to insert a loxP site upstream of exon 1, and a loxP-flanked neo cassette (in reverse orientation) just downstream of exon 1 of the targeted gene. The construct was electroporated into 129S1/Sv-derived CJ7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with C57BL/6 females to establish the colony. The TANK1^flox Mice were then bred with EIIa-cre mice (C57BL/6 congenic background; similar to Stock No. 003724). The resulting mice lacking both exon 1 and the floxed neo cassette (TANK1^-) were selectively bred to remove the EIIa-cre transgene. The donating investigator reports that heterozygous mice were backcrossed to C57BL/6NCr for at least 10 generations, and then bred together to generate homozygotes. Homozygous embryos were sent to The Jackson Laboratory Repository.

Allele

Symbol: Tnks^tm1.1Yjc
Name: targeted mutation 1.1, Y Jeffrey Chiang
MGI accession ID: MGI:3804704
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Tnks
Marker name: tankyrase, TRF1-interacting ankyrin-related ADP-ribose polymerase
Chromosome: 8
Strain of origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Molecular note: Exon 1 was removed by germ line, cre-mediated recombination. The absence of protein expression was confirmed by western blot analysis on testis, kidney and spleen extracts.