This congneic strain has the Fscn2ahl8 allele backcrossed from DAB/2J onto C57BL/6J.
This C57BL/6J subcongenic line with the DBA/2J-derived Fscn2ahl8 mutation was developed by first crossing mice of the B6.D2-Chr11D/LusJ congenic strain (Davis et al., 2005) with C57BL/6J mice. The hybrid progeny from this cross were then mated with C57BL/6J mice, and the resulting backcross progeny were genotyped for five DNA microsatellite markers spanning the introgressed region of the B6.D2-Chr11D/LusJ congenic strain. Backcross mice that were homozygous for C57BL/6J alleles at markers D11Mit90, D11Mit360, D11Mit338, and D11Mit203, and heterozygous for C57BL/6J and DBA/2J alleles at D11Mit104 were selected and interbred. The progeny from these matings were genotyped and those that were confirmed to be homozygous for C57BL/6J alleles at D11Mit90 (70.5 Mb position of Chr 11, GRCm38), D11Mit360 (103.4 Mb), D11Mit338 (115.5 Mb), and D11Mit203 (116.4 Mb), and homozygous for DBA/2J alleles at D11Mit104 (119.3) and Fscn2 (120.4 Mb) were interbred to produce this homozygous subcongenic line. This strain is maintained by sibling inbreeding homozygotes.
|Allele Name||age related hearing loss 18|
|Gene Symbol and Name||Fscn2, fascin actin-bundling protein 2|
|Strain of Origin||DBA/2J|
|General Note||ahl8 interacts with Cdh23 |
|Molecular Note||G to A transition located in exon 1 and is of predicted to cause a nonsynonymous amino acid change of arginine to histidine at position 109 (R109H). The known genealogy of the DBA-related strains and genotyping analysis of archived DBA/2J DNA samples indicate that this allele occurred in the DBA/2J lineage between 1951 (when it was separated from the DBA/2N lineage) and 1975.|
When using the B6.D2-Fscn2ahl8/4Kjn mouse strain in a publication, please cite the originating article(s) and include JAX stock #009629 in your Materials and Methods section.