The Arntl knock-out strain may be useful in studies of circadian rhythm, arthritis, ankylosis, and glucose homeostasis.
Dr. Joseph S. Takahashi, Univ Texas Southwestern Medical Ctr
These targeted mutation mice exhibit a loss of both behavioral and molecular circadian rhythms. When placed in constant darkness, the mice undergo an immediate loss of circadian rhythmicity. Locomotor activity is impaired in both light and constant dark cycles.
Reduced total activity is seen as the mice age. They display a progressive noninflammatory arthropathy. Little pathology is seen prior to 11 weeks of age, but virtually all homozygotes develop joint ankylosis due to flowing ossification of ligaments and tendons by 35 weeks of age. Bone density and articular cartilage are unaffected.
Inactivation of the gene suppresses diurnal variation in glucose and triglycerides. Gluconeogenesis is abolished in homozygotes, but the counterregulatory response of corticosterone and glucagon to insulin-induced hypoglycemia is retained.
Homozygotes are viable but not fertile and have an increased mortality rate after 26 weeks of age. A truncated, non-functional protein is produced. This strain may be useful in studies of circadian rhythm, arthritis, ankylosis, and glucose homeostasis.
The helix-loop-helix domain within exon 4 and all of exon 5 were replaced with a neomycin resistance gene cassette. 129/Sv-derived GS1 embryonic stem (ES) cells were used to create the mutation. This line was backcrossed 14 times to C57BL/6 by the donating laboratory.
|Allele Name||targeted mutation 1, Christopher A Bradfield|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Arntltm1Bra; targeted mutation 1, Christopher A Bradfield|
|Gene Symbol and Name||Arntl, aryl hydrocarbon receptor nuclear translocator-like|
|Gene Synonym(s)||Arnt3; bHLHe5; BMAL1; BMAL1c; TIC; MOP3; PASD3; JAP3; Bmal1|
|Strain of Origin||129/Sv|
|Molecular Note||A portion of exon 4 containing the helix-loop-helix coding domain and all of exon 5 were replaced by a neomycin selection cassette. A larger transcript, resulting from a cryptic splice site between exon 3 and the neomycin resistance gene, was detected by Northern blot analysis. The polypeptide truncates 15 residues after this splice site, prior to the functional domains encoded by exon 4 in the wild-type locus.|
|Mutations Made By|| |
Christopher Bradfield, University of Wisconsin-Madison
When maintained as a live colony, hetetozygotes may be bred. It is recommended that females be disturbed as little as possible because they are prone to killing their litters. Homozygotes are viable, but both males and females are reportedly sterile.
When using the Mop3- mouse strain in a publication, please cite the originating article(s) and include JAX stock #009100 in your Materials and Methods section.
|Heterozygous or Wild-Type for Arntl<tm1Bra>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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