STOCK Ednrb^tm1Nrd/J

Stock number: 009063
Research areas: Developmental Biology Research, Mouse/Human Gene Homologs, Neurobiology Research, Research Tools

Description

These Ednrb^flex3 mutant mice harbor a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the Ednrb (endothelin receptor type B or ET-B receptor) gene and may be useful in generating conditional mutations for studying the role of Ednrb in development of melanocytes, development of neurons and glia of the enteric nervous system, neural crest-derived cells, mesenchymal-derived smooth muscle cells, vasodilation, mitogen signaling and cancer, and human Hirschsprung's disease (HSCR).

Detailed description

Mice heterozygous for this Ednrb^flex3 allele are viable and fertile, with a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the Ednrb (endothelin receptor type B or ET-B receptor) gene. When bred to mice that express Cre recombinase, the resulting offspring can have one of three resulting genotypes in the cre-expressing tissue(s); only the neo selection cassette deleted, only exon 3 deleted, or both the neo selection cassette and exon 3 deleted. The two latter genotypes are expected to result in a frameshifted transcript that is reported to confer the null phenotype. These mutant mice may be useful in generating conditional mutations for studying the role of Ednrb in development of melanocytes, development of neurons and glia of the enteric nervous system, neural crest-derived cells, mesenchymal-derived smooth muscle cells, vasodilation, mitogen signaling and cancer, and human Hirschsprung's disease (HSCR).

When bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of neural crest development.

Development

A targeting vector was designed to place a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the targeted gene. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6 females. The resulting Ednrb^flex3 mutant mice were then bred to Wnt1-Cre mice (reported to be C57BL/6J genetic background (although they were white); see Stock No. 007807). Double mutant mice were bred together or to Ednrb^flex3 single mutant mice for approximately four years prior to arrival at The Jackson Laboratory. Upon arrival, Ednrb^flex3 mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to confirm the Wnt1-Cre transgene was removed.

Allele

Symbol: Ednrb^tm1Nrd
Name: targeted mutation 1, Noah Druckenbrod
MGI accession ID: MGI:3813973
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Ednrb
Marker name: endothelin receptor type B
Chromosome: 14
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Expressed genes: neo,neomycin resistance,bacterial
Molecular note: A floxed neo cassette was placed upstream of exon 3 and an additional loxP site was inserted downstream of exon 3.