These Ednrbflex3 mutant mice harbor a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the Ednrb (endothelin receptor type B or ET-B receptor) gene and may be useful in generating conditional mutations for studying the role of Ednrb in development of melanocytes, development of neurons and glia of the enteric nervous system, neural crest-derived cells, mesenchymal-derived smooth muscle cells, vasodilation, mitogen signaling and cancer, and human Hirschsprung's disease (HSCR).
Noah Druckenbrod, University Of Wisconsin
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Ednrb | endothelin receptor type B |
Mice heterozygous for this Ednrbflex3 allele are viable and fertile, with a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the Ednrb (endothelin receptor type B or ET-B receptor) gene. When bred to mice that express Cre recombinase, the resulting offspring can have one of three resulting genotypes in the cre-expressing tissue(s); only the neo selection cassette deleted, only exon 3 deleted, or both the neo selection cassette and exon 3 deleted. The two latter genotypes are expected to result in a frameshifted transcript that is reported to confer the null phenotype. These mutant mice may be useful in generating conditional mutations for studying the role of Ednrb in development of melanocytes, development of neurons and glia of the enteric nervous system, neural crest-derived cells, mesenchymal-derived smooth muscle cells, vasodilation, mitogen signaling and cancer, and human Hirschsprung's disease (HSCR).
When bred to a strain expressing Cre recombinase in midbrain/dorsal spinal cord (see Stock No. 009107 for example), this mutant mouse strain may be useful in studies of neural crest development.
A targeting vector was designed to place a loxP-flanked neo cassette upstream of exon 3, as well as a loxP site downstream of exon 3 of the targeted gene. This construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric males were bred with C57BL/6 females. The resulting Ednrbflex3 mutant mice were then bred to Wnt1-Cre mice (reported to be C57BL/6J genetic background (although they were white); see Stock No. 007807). Double mutant mice were bred together or to Ednrbflex3 single mutant mice for approximately four years prior to arrival at The Jackson Laboratory. Upon arrival, Ednrbflex3 mutant mice were bred to C57BL/6J inbred mice (Stock No. 000664) for at least one generation to confirm the Wnt1-Cre transgene was removed.
Expressed Gene | neo, neomycin resistance, bacterial |
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Site of Expression |
Allele Name | targeted mutation 1, Noah Druckenbrod |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | Ednrbflex3 |
Gene Symbol and Name | Ednrb, endothelin receptor type B |
Gene Synonym(s) | |
Expressed Gene | neo, neomycin resistance, bacterial |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 14 |
Molecular Note | A floxed neo cassette was placed upstream of exon 3 and an additional loxP site was inserted downstream of exon 3. |
Mutations Made By | Noah Druckenbrod, University Of Wisconsin |
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Ednrbtm1Nrd/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #009063 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Ednrb<tm1Nrd>, 1 pair minimum |
Frozen Mouse Embryo | STOCK Ednrb<tm1Nrd>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Ednrb<tm1Nrd>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | STOCK Ednrb<tm1Nrd>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | STOCK Ednrb<tm1Nrd>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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