Homozygous Cd2ap, CD2-associated protein targeted mutant mice die at approximately 6 weeks of age. Cardiac hypertrophy, splenic and thymic atrophy, and ascites may be found upon postmortem examination. Proteinuria can be detected as early as 2 weeks of age, indicating kidney dysfunction. Heterozygotes show increased susceptibility to glomerular injury by nephrotoxic antibodies or immune complexes.
Dr. Andrey Shaw, Washington University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cd2ap | CD2-associated protein |
Homozygous targeted mutant mice begin to show growth retardation at about 3 weeks of age, and die at approximately 6 weeks. Cardiac hypertrophy, splenic and thymic atrophy, and ascites may be found upon postmortem examination. Proteinuria can be detected as early as 2 weeks of age, indicating kidney dysfunction. The predominant kidney pathology involves glomeruli which become sclerotic by 4 weeks. CD2AP protein is not detected in the thymus, kidney, liver or spleen of homozygous targeted animals. Naive and cultured T cells show increased sensitivity to antigen, increased proliferation, increased number of cell divisions and increased apoptosis compared to wild type cells. Heterozygotes show increased susceptibility to glomerular injury by nephrotoxic antibodies or immune complexes.
Exon 2, encoding the first SRC homology 3 (SH3) domain, was replaced with a neomycin resistance cassette using 129X1/SvJ-derived RW-4 embryonic stem (ES) cells. Mice were backcrossed to C57BL/6 fifteen times by the donating laboratory.
Allele Name | targeted mutation 1, Andrey S Shaw |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Cd2ap- |
Gene Symbol and Name | Cd2ap, CD2-associated protein |
Gene Synonym(s) | |
Strain of Origin | 129X1/SvJ |
Chromosome | 17 |
General Note | Heterozygous mutant mice exhibit highly organized microtubular deposits in the mesangium which is characteristic in human immunotactoid glomerulopathy (J:84748). |
Molecular Note | The sequences encoding the first SRC homology 3 domain was replaced with a neomycin-resistance gene. Immunoblots of kidney, liver, spleen, and thymus proteins from homozygous mice indicated the absence of gene product. |
Mutations Made By | Dr. Andrey Shaw, Washington University School of Medicine |
When maintained as a live colony, heterozygotes may be bred. Homozygotes die at 6-7 weeks of age.
When using the B6.129X1-Cd2aptm1Shaw/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008907 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous or Wild-type for Cd2ap<tm1Shaw> |
Frozen Mouse Embryo | B6.129X1-Cd2ap<tm1Shaw>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Cd2ap<tm1Shaw>/J | $2595.00 |
Frozen Mouse Embryo | B6.129X1-Cd2ap<tm1Shaw>/J | $3373.50 |
Frozen Mouse Embryo | B6.129X1-Cd2ap<tm1Shaw>/J | $3373.50 |
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