On this C57BL/6J congenic background homozygotes for Rd3rd3 have normal photoreceptor development then retinal degeneration begins at 4 weeks of age with complete loss of photoreceptor cells by 3 months of age.
The Rd3rd3 allele was backcrossed onto C57BL/6J from STOCK In(5)30Rk/J, on which it was first identified. This was done by 5 cycles of backcross-intercross breeding to reach an incipient congenic then intercrossing to homozygosity. This strain is maintained by sibling inbreeding homozygotes.
|Allele Name||retinal degeneration 3|
|Gene Symbol and Name||Rd3, retinal degeneration 3|
|Strain of Origin||RBF/DnJ|
|General Note||This mutation causes retinal degeneration. In homozygous mutant mice, development proceeds normally through the second postnatal week. Thereafter, photoreceptor and outer nuclear layers begin to degenerate, and by 8 weeks, no photoreceptor cells remain. Changes in electroretinograms parallel the histologic changes. As of 1998 this is the only type of retinal degeneration reported in mice in which the photoreceptors are initially normal (J:4367).|
|Molecular Note||A C to T substitution in Rd3 results in a stop codon after residue 106. The truncated protein is initially expressed in in vitro assays but is degraded.|