Homozygous protocadherin 21 targeted mutation mice are viable and fertile and are normal in size and viability. They exhibit a slow retinal degeneration in which the outer segments of both rods and cones are disorganized and fragmented.
Jeremy Nathans, Johns Hopkins University
Homozygous protocadherin 21 targeted mutation mice are viable and fertile and are normal in size and viability. They exhibit a slow retinal degeneration in which the outer segments of both rods and cones are disorganized and fragmented. Light responses of both rods and cones is only modestly compromised and phototransduction proteins appear to be correctly localized. This strain may be useful in studies of sensory neurons/photoreceptors, and retinal degeneration.
Exons 10-17 of the gene were replaced with a PGKneo selectable marker using the (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cell line. This strain was maintained on a mixed C57BL/6 and 129 background by the donating laboratory.
|Allele Name||targeted mutation 1, Jeremy Nathans|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Cdhr1, cadherin-related family member 1|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A neomycin selection cassette replaced exons 10-17, which encode the C-terminal two thirds of the protein. Northern blot and immunohistochemical analysis confirmed the absence of encoded transcript and protein, respectively.|
When maintained as a live colony, homozygotes may be bred.
When using the B6;129-Cdhr1tm1Nat/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008616 in your Materials and Methods section.