This solute carrier family protein localizes to the inner mitochondrial membrane where it is essential for appropriate iron importation. Homozygotes for this null allele fail to incorporate iron into heme, develop profound embryonic hypochromic anemia and all die by E11.5. Porphyria is not found in these mice. This strain has been used to assess the role of other proteins in the heme regulatory pathway.
This gene trap mutation was made in the laboratory of Luanne Peters using E14 ES cell clone XB454 from Bay Genomics. This mutation was backcrossed onto the C57BL/6J background and sperm was frozen from heterozygous males at generation N10.