| Allele Name | transgene insertion L2G85, Christopher H Contag |
| Allele Type | Transgenic (Reporter) |
| Allele Synonym(s) | L2685; L2G85; luc+; Tg (CAG- Luc, GFP) |
| Gene Symbol and Name | Tg(CAG-luc,-GFP)L2G85Chco, transgene insertion L2G85, Christopher H Contag |
| Gene Synonym(s) | |
| Promoter | ACTB, actin, beta, chicken |
|---|
| Promoter | CMV, cytomegalovirus, human |
|---|
| Expressed Gene | GFP, Green Fluorescent Protein, |
|---|
| Expressed Gene | luc, luciferase, firefly |
|---|
| Site of Expression | Widespread expression of firefly luciferase is reported in all tissues and organs, except mature erythrocytes. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin. |
|---|
| Strain of Origin | FVB |
| Chromosome | 7 |
| General Note | Fine mapping performed at The Jackson Laboratory indicates that the transgene integrated onto the proximal end of Chr. 7 and the congenic interval extends from D7Mit306 (10 Mb) through D7Jmp8 (29 Mb). |
| Molecular Note | A transgenic construct containing a modified firefly luciferase gene open reading frame, 54bp of the foot and mouth disease virus (FMDV) 2A sequence (ribsome slippage site) and eGFP sequence under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer (CAG), was injected into fertilized FVB mouse eggs. Founder line L2G85 animals were bred to wildtype FVB mice. |
| Mutations Made By | Ryan McBride Spitler, Stanford University |
