Mice harboring the CAG-luc-eGFP L2G85 transgene exhibit expression of firefly luciferase directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). These CAG-luc-eGFP L2G85 transgenic mice may be useful in studies of transplantation, noninvasive lineage mapping, in vivo bioluminescence imaging and technology development.
Christopher H Contag, Stanford University
Genetic Background | Generation |
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N21+N3F2
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Allele Type |
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Transgenic (Reporter) |
Mice homozygous for the CAG-luc-eGFP L2G85 transgene are viable and fertile, with expression of firefly luciferase and enhanced green fluorescence protein directed by the CAG promoter (human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter). Bioluminesence is detected in heart, spleen, muscle, pancreas, skin, thymus and bone marrow. Luciferase activity is not detected in mature erythrocytes, although low levels are detected in erythrocyte precursors and varying levels of activity in all leukocyte subsets tested. Homozygotes have no reported gross physical or behavioral abnormalities. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin (upper epidermal layers) by fluorescence microscopy. The Donating Investigator reports that for the FVB-Tg(CAG-luc,-GFP)L2G85Chco/J strain (Stock No. 008450), no GFP fluorescence is detected in hematopoietic tissues by flow cytometric analysis.
The CAG-luc-eGFP transgenic construct was designed by the laboratory of Dr. Christopher H. Contag (Stanford University School of Medicine). Specifically, the transgene contained the human cytomegalovirus immediate early promoter enhancer with chicken beta-actin/rabbit beta-globin hybrid promoter (CAG; from the pCAGGS vector) upstream of a modified firefly luciferase gene open reading frame, 54 bp of the foot and mouth disease virus (FMDV) 2A sequence (ribsome slippage site), 24 bp polylinker, and enhanced Green Fluorescent Protein (eGFP) open reading frame. This transgene was injected into fertilized FVB mouse eggs. Founder line L2G85 animals were bred to wildtype FVB mice. These FVB-L2G85 (or FVB.luc) transgenic were then backcrossed to FVB for 20 generations prior to arrival at The Jackson Laboratory. Upon arrival, CAG-luc-eGFP transgenic mice were bred with FVB/NJ inbred mice (Stock No. 001800) for at least one generation to establish the colony.
Fine mapping performed at The Jackson Laboratory would indicate that the transgene integrated onto the proximal end of chromosome 7 - somewhere between D7Mit306 (~10 Mb) and D7Jmp8 (~29 Mb).
Expressed Gene | GFP, Green Fluorescent Protein, |
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Expressed Gene | luc, luciferase, firefly |
Site of Expression | Widespread expression of firefly luciferase is reported in all tissues and organs, except mature erythrocytes. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin. |
Allele Name | transgene insertion L2G85, Christopher H Contag |
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Allele Type | Transgenic (Reporter) |
Allele Synonym(s) | L2685; L2G85; luc+; Tg (CAG- Luc, GFP) |
Gene Symbol and Name | Tg(CAG-luc,-GFP)L2G85Chco, transgene insertion L2G85, Christopher H Contag |
Gene Synonym(s) | |
Promoter | ACTB, actin, beta, chicken |
Promoter | CMV, cytomegalovirus, human |
Expressed Gene | GFP, Green Fluorescent Protein, |
Expressed Gene | luc, luciferase, firefly |
Site of Expression | Widespread expression of firefly luciferase is reported in all tissues and organs, except mature erythrocytes. Following luciferin injection, luciferase expression is generally greater in males than females. GFP fluorescence is detected in skin. |
Strain of Origin | FVB |
Chromosome | 7 |
General Note | Fine mapping performed at The Jackson Laboratory indicates that the transgene integrated onto the proximal end of Chr. 7 and the congenic interval extends from D7Mit306 (10 Mb) through D7Jmp8 (29 Mb). |
Molecular Note | A transgenic construct containing a modified firefly luciferase gene open reading frame, 54bp of the foot and mouth disease virus (FMDV) 2A sequence (ribsome slippage site) and eGFP sequence under the control of the chicken beta actin promoter coupled with the cytomegalovirus (CMV) immediate early enhancer (CAG), was injected into fertilized FVB mouse eggs. Founder line L2G85 animals were bred to wildtype FVB mice. |
Mutations Made By | Ryan McBride Spitler, Stanford University |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the L2G85 mouse strain in a publication, please cite the originating article(s) and include JAX stock #008450 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous for Tg(CAG-luc,-GFP)L2G85Chco |
Frozen Mouse Embryo | FVB-Tg(CAG-luc -GFP)L2G85Chco/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(CAG-luc -GFP)L2G85Chco/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | FVB-Tg(CAG-luc -GFP)L2G85Chco/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | FVB-Tg(CAG-luc -GFP)L2G85Chco/J Frozen Embryo | $3373.50 |
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