These synaptogamin 3 and 6 knock-out and synaptotagmin 5 and 10 knock-in quadruple targeted mutation mice have no overt phenotype, but may be useful in further characterizing these genes and examining potential roles in vesicular trafficking and signal transduction. No overt phenotype has been reported.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Syt6 | synaptotagmin VI |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Syt5 | synaptotagmin V |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Syt3 | synaptotagmin III |
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | Syt10 | synaptotagmin X |
These synaptogamin 3 and 6 knock-out and synaptotagmin 5 and 10 knock-in quadruple targeted mutation mice have no overt phenotype, but may be useful in further characterizing these genes and examining potential roles in vesicular trafficking and signal transduction. No overt phenotype has been reported.
The Syt5 knock-in was created by inserting a tetracysteine motif tag dimer (Flag-CCPGCC-Flag-Flag-CCPGCC-Flag) in exon 3 of the gene. Exon 3 was additionally flanked by loxP sites and an Frt-flanked neomycin resistance cassette was placed 3' of the exon. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-{\i Kitl{\up +}}-derived R1 embryonic stem (ES) cells. The donating laboratory may have removed the neomycin cassette.
The Syt 10 knock-in was created by inserting a tetracysteine tag (Flag-C(4)-Flag) in exon 2 of the gene. Exon 2 was additionally flanked by loxP sites. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-{\i Kitl{\up +}}-derived R1 embryonic stem (ES) cells. An Flp-neomycin-Flp cassette placed in intron 1 has been removed.
The Syt3 knock-out was created through the insertion of a neomycin resistance cassette in exon 1 (amino acid 220 of NCBI protein NP_061995; bp 3738 of NCBI GeneID:20981).
The Syt6 knock-out was created through the insertion of a neomycin resistance cassette in amino acid 964/ bp 10938.
The four independently-created mutations were intercrossed to create the quadruple-mutant mice. It is probable that (129X1/SvJ x 129S1/Sv)F1-Kitl+ -derived R1 embryonic stem (ES) cells were used to create these mutations with chimera's crossed to C57BL/6.
Allele Name | targeted mutation 1, Thomas C Sudhof |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Syt6, synaptotagmin VI |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 3 |
Molecular Note | The Syt6 knock-out was created through the insertion of a neomycin resistance cassette in amino acid 964/ bp 10938. |
Allele Name | targeted mutation 1, Thomas C Sudhof |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Syt5, synaptotagmin V |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 7 |
Molecular Note | he Syt5 knock-in was created by inserting a tetracysteine motif tag dimer (Flag-CCPGCC-Flag-Flag-CCPGCC-Flag) in exon 3 of the gene. Exon 3 was additionally flanked by loxP sites and an Frt-flanked neomycin resistance cassette was placed 3' of the exon. The neomycin fragment may have been removed. |
Mutations Made By | Dr. Thomas Sudhof, Stanford University School of Medicine |
Allele Name | targeted mutation 1, Thomas C Sudhof |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | |
Gene Symbol and Name | Syt3, synaptotagmin III |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 7 |
Molecular Note | The Syt3 knock-out was created through the insertion of a neomycin resistance cassette in exon 1 (amino acid 220 of NCBI protein NP_061995; bp 3738 of NCBI GeneID:20981). |
Allele Name | targeted mutation 1, Thomas C Sudhof |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | |
Gene Symbol and Name | Syt10, synaptotagmin X |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 15 |
Molecular Note | The Syt 10 allele was created by inserting a tetracysteine tag (Flag-C(4)-Flag) in exon 2 of the gene. Exon 2 was additionally flanked by loxP sites. An Flp-neomycin-Flp cassette placed in intron 1 has been removed. |
When maintained as a live colony, quadruple homozygous mutant mice may be intercrossed.
When using the AMK mouse strain in a publication, please cite the originating article(s) and include JAX stock #008413 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Syt6<tm1Sud>, Homozygous for Syt5<tm1Sud>, Homozygous for Syt3<tm1Sud>, Homozygous for Syt10<tm1Sud> |
Frozen Mouse Embryo | B6;129-Syt6<tm1Sud> Syt5<tm1Sud> Syt3<tm1Sud> Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129-Syt6<tm1Sud> Syt5<tm1Sud> Syt3<tm1Sud> Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6;129-Syt6<tm1Sud> Syt5<tm1Sud> Syt3<tm1Sud> Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6;129-Syt6<tm1Sud> Syt5<tm1Sud> Syt3<tm1Sud> Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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