Overview

Also Known As:AMK

These synaptogamin 3 and 6 knock-out and synaptotagmin 5 and 10 knock-in quadruple targeted mutation mice have no overt phenotype, but may be useful in further characterizing these genes and examining potential roles in vesicular trafficking and signal transduction. No overt phenotype has been reported.

Donating Investigator

Dr. Thomas C. Sudhof, Stanford University School of Medicine

Genetic overview

Genetic Background	Generation

Syt6^tm1Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Syt6	synaptotagmin VI

Syt5^tm1Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Syt5	synaptotagmin V

Syt3^tm1Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Syt3	synaptotagmin III

Syt10^tm1Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), No functional change)	Syt10	synaptotagmin X

View Genetics

Research Applications

Neurobiology Research

View All Research Applications

Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

View Price List

Details

Detailed Description

These synaptogamin 3 and 6 knock-out and synaptotagmin 5 and 10 knock-in quadruple targeted mutation mice have no overt phenotype, but may be useful in further characterizing these genes and examining potential roles in vesicular trafficking and signal transduction. No overt phenotype has been reported.

Development

The Syt5 knock-in was created by inserting a tetracysteine motif tag dimer (Flag-CCPGCC-Flag-Flag-CCPGCC-Flag) in exon 3 of the gene. Exon 3 was additionally flanked by loxP sites and an Frt-flanked neomycin resistance cassette was placed 3' of the exon. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-{\i Kitl{\up +}}-derived R1 embryonic stem (ES) cells. The donating laboratory may have removed the neomycin cassette.

The Syt 10 knock-in was created by inserting a tetracysteine tag (Flag-C(4)-Flag) in exon 2 of the gene. Exon 2 was additionally flanked by loxP sites. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-{\i Kitl{\up +}}-derived R1 embryonic stem (ES) cells. An Flp-neomycin-Flp cassette placed in intron 1 has been removed.

The Syt3 knock-out was created through the insertion of a neomycin resistance cassette in exon 1 (amino acid 220 of NCBI protein NP_061995; bp 3738 of NCBI GeneID:20981).

The Syt6 knock-out was created through the insertion of a neomycin resistance cassette in amino acid 964/ bp 10938.

The four independently-created mutations were intercrossed to create the quadruple-mutant mice. It is probable that (129X1/SvJ x 129S1/Sv)F1-Kitl⁺ -derived R1 embryonic stem (ES) cells were used to create these mutations with chimera's crossed to C57BL/6.

Genetics

Syt6^tm1Sud

Allele Symbol: Syt6^tm1Sud

Allele Name	targeted mutation 1, Thomas C Sudhof
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Syt6, synaptotagmin VI
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	3
Molecular Note	The Syt6 knock-out was created through the insertion of a neomycin resistance cassette in amino acid 964/ bp 10938.

Syt5^tm1Sud

Allele Symbol: Syt5^tm1Sud

Allele Name	targeted mutation 1, Thomas C Sudhof
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)
Gene Symbol and Name	Syt5, synaptotagmin V
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	7
Molecular Note	he Syt5 knock-in was created by inserting a tetracysteine motif tag dimer (Flag-CCPGCC-Flag-Flag-CCPGCC-Flag) in exon 3 of the gene. Exon 3 was additionally flanked by loxP sites and an Frt-flanked neomycin resistance cassette was placed 3' of the exon. The neomycin fragment may have been removed.
Mutations Made By	Dr. Thomas Sudhof, Stanford University School of Medicine

Syt3^tm1Sud

Allele Symbol: Syt3^tm1Sud

Allele Name	targeted mutation 1, Thomas C Sudhof
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)
Gene Symbol and Name	Syt3, synaptotagmin III
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	7
Molecular Note	The Syt3 knock-out was created through the insertion of a neomycin resistance cassette in exon 1 (amino acid 220 of NCBI protein NP_061995; bp 3738 of NCBI GeneID:20981).

Syt10^tm1Sud

Allele Symbol: Syt10^tm1Sud

Allele Name	targeted mutation 1, Thomas C Sudhof
Allele Type	Targeted (Conditional ready (e.g. floxed), No functional change)
Allele Synonym(s)
Gene Symbol and Name	Syt10, synaptotagmin X
Gene Synonym(s)
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	15
Molecular Note	The Syt 10 allele was created by inserting a tetracysteine tag (Flag-C(4)-Flag) in exon 2 of the gene. Exon 2 was additionally flanked by loxP sites. An Flp-neomycin-Flp cassette placed in intron 1 has been removed.

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Neurotransmitter Receptor and Synaptic Vesicle Defects

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: Syt10^tm1Sud/Syt10^tm1Sud Syt3^tm1Sud/Syt3^tm1Sud Syt5^tm1Sud/Syt5^tm1Sud Syt6^tm1Sud/Syt6^tm1Sud
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

no abnormal phenotype detected
- Normal - quadruple mutant animals display no overt phenotype

References

Additional - Syt10^tm1Sud related

Additional - Syt3^tm1Sud related

Additional - Syt5^tm1Sud related

Additional - Syt6^tm1Sud related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Syt3
- Standard PCR:Syt6
- Separated PCR:Syt5
- Standard PCR:Syt10
- Genotyping resources and troubleshooting
Breeding Considerations

When maintained as a live colony, quadruple homozygous mutant mice may be intercrossed.
- Additional Breeding and Husbandry Support
Citation
When using the AMK mouse strain in a publication, please cite the originating article(s) and include JAX stock #008413 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Homozygous for Syt6<tm1Sud>, Homozygous for Syt5<tm1Sud>, Homozygous for Syt3<tm1Sud>, Homozygous for Syt10<tm1Sud>

Related Products and Services

Frozen Mouse Embryo	B6;129-Syt6<tm1Sud> Syt5<tm1Sud> Syt3<tm1Sud> Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6;129-Syt6<tm1Sud> Syt5<tm1Sud> Syt3<tm1Sud> Frozen Embryos	$2595.00
Frozen Mouse Embryo	B6;129-Syt6<tm1Sud> Syt5<tm1Sud> Syt3<tm1Sud> Frozen Embryos	$3373.50
Frozen Mouse Embryo	B6;129-Syt6<tm1Sud> Syt5<tm1Sud> Syt3<tm1Sud> Frozen Embryos	$3373.50

Payment Terms and Conditions

Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

Terms Of Use

General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Use of MICE by companies or for-profit entities requires a license prior to shipping.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:AMK

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Syt6tm1Sud

Allele Symbol: Syt6tm1Sud

Syt5tm1Sud

Allele Symbol: Syt5tm1Sud

Syt3tm1Sud

Allele Symbol: Syt3tm1Sud

Syt10tm1Sud

Allele Symbol: Syt10tm1Sud

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Syt10tm1Sud/Syt10tm1Sud Syt3tm1Sud/Syt3tm1Sud Syt5tm1Sud/Syt5tm1Sud Syt6tm1Sud/Syt6tm1Sudinvolves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

References

Additional - Syt10tm1Sud related

Additional - Syt3tm1Sud related

Additional - Syt5tm1Sud related

Additional - Syt6tm1Sud related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Syt6^tm1Sud

Allele Symbol: Syt6^tm1Sud

Syt5^tm1Sud

Allele Symbol: Syt5^tm1Sud

Syt3^tm1Sud

Allele Symbol: Syt3^tm1Sud

Syt10^tm1Sud

Allele Symbol: Syt10^tm1Sud

Genotype: Syt10^tm1Sud/Syt10^tm1Sud Syt3^tm1Sud/Syt3^tm1Sud Syt5^tm1Sud/Syt5^tm1Sud Syt6^tm1Sud/Syt6^tm1Sud
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Additional - Syt10^tm1Sud related

Additional - Syt3^tm1Sud related

Additional - Syt5^tm1Sud related

Additional - Syt6^tm1Sud related