B6;129-Syt6^tm1Sud Syt5^tm1Sud Syt3^tm1Sud Syt10^tm1Sud/J

Stock number: 008413
Product name: AMK
Research areas: Neurobiology Research

Description

These synaptogamin 3 and 6 knock-out and synaptotagmin 5 and 10 knock-in quadruple targeted mutation mice have no overt phenotype, but may be useful in further characterizing these genes and examining potential roles in vesicular trafficking and signal transduction. No overt phenotype has been reported.

Detailed description

These synaptogamin 3 and 6 knock-out and synaptotagmin 5 and 10 knock-in quadruple targeted mutation mice have no overt phenotype, but may be useful in further characterizing these genes and examining potential roles in vesicular trafficking and signal transduction. No overt phenotype has been reported.

Development

The Syt5 knock-in was created by inserting a tetracysteine motif tag dimer (Flag-CCPGCC-Flag-Flag-CCPGCC-Flag) in exon 3 of the gene. Exon 3 was additionally flanked by loxP sites and an Frt-flanked neomycin resistance cassette was placed 3' of the exon. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-{\i Kitl{\up +}}-derived R1 embryonic stem (ES) cells. The donating laboratory may have removed the neomycin cassette.

The Syt 10 knock-in was created by inserting a tetracysteine tag (Flag-C(4)-Flag) in exon 2 of the gene. Exon 2 was additionally flanked by loxP sites. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-{\i Kitl{\up +}}-derived R1 embryonic stem (ES) cells. An Flp-neomycin-Flp cassette placed in intron 1 has been removed.

The Syt3 knock-out was created through the insertion of a neomycin resistance cassette in exon 1 (amino acid 220 of NCBI protein NP_061995; bp 3738 of NCBI GeneID:20981).

The Syt6 knock-out was created through the insertion of a neomycin resistance cassette in amino acid 964/ bp 10938.

The four independently-created mutations were intercrossed to create the quadruple-mutant mice. It is probable that (129X1/SvJ x 129S1/Sv)F1-Kitl⁺ -derived R1 embryonic stem (ES) cells were used to create these mutations with chimera's crossed to C57BL/6.

Allele

Symbol: Syt10^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:3799077
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Syt10
Marker name: synaptotagmin X
Chromosome: 15
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: The Syt 10 allele was created by inserting a tetracysteine tag (Flag-C(4)-Flag) in exon 2 of the gene. Exon 2 was additionally flanked by loxP sites. An Flp-neomycin-Flp cassette placed in intron 1 has been removed.

Allele

Symbol: Syt3^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:3799075
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Syt3
Marker name: synaptotagmin III
Chromosome: 7
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: The Syt3 knock-out was created through the insertion of a neomycin resistance cassette in exon 1 (amino acid 220 of NCBI protein NP_061995; bp 3738 of NCBI GeneID:20981).

Allele

Symbol: Syt6^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:3799072
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Syt6
Marker name: synaptotagmin VI
Chromosome: 3
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: The Syt6 knock-out was created through the insertion of a neomycin resistance cassette in amino acid 964/ bp 10938.

Allele

Symbol: Syt5^tm1Sud
Name: targeted mutation 1, Thomas C Sudhof
MGI accession ID: MGI:3799073
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Syt5
Marker name: synaptotagmin V
Chromosome: 7
Strain of origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Molecular note: he Syt5 knock-in was created by inserting a tetracysteine motif tag dimer (Flag-CCPGCC-Flag-Flag-CCPGCC-Flag) in exon 3 of the gene. Exon 3 was additionally flanked by loxP sites and an Frt-flanked neomycin resistance cassette was placed 3' of the exon. The neomycin fragment may have been removed.