These targeted mutant (knock-in line ADF) mice carry an insertion incorporating a truncated exon 16 of the mouse amyloid beta (A4) precursor protein (App) gene as well as the Swedish mutant exon 16 and London/Dutch mutations of exon 17. Truncated exon 16 is incorporated into the sAPPbeta-FLAG transcript, but exon 17 is not expressed. No overt phenotype has been observed. Homozygotes are viable and fertile. This strain may be useful in studies of Alzheimer's disease.
Dr. Thomas C. Sudhof, Stanford University School of Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence) | App | amyloid beta (A4) precursor protein |
These targeted mutant (knock-in line ADF) mice carry an insertion incorporating a truncated exon 16 of the mouse amyloid beta (A4) precursor protein (App) gene as well as the Swedish mutant exon 16 and London/Dutch mutations of exon 17. Truncated exon 16 is incorporated into the sAPPbeta-FLAG transcript, but exon 17 is not expressed. No overt phenotype has been observed. Homozygotes are viable and fertile. This strain may be useful in studies of Alzheimer's disease.
The first portion of the targeting vector incorporates a wildtype mouse App exon 16 (truncated after residue KM) followed by a FLAG tag (2 repeats), a stop codon, a poly A signal region from the human growth hormone gene, and a pair of neomycin resistance cassettes which are flanked by a pair of FRT sites. All of these elements are flanked by a single pair or loxP sites. This construct is followed by an additional copy of exon 16 carrying the Swedish mutation and modified version of exon 17 carrying the London mutation plus the Dutch mutation.
This vector was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl+-derived R1 embryonic stem (ES) cells. Resultant mice were crossed with an FLP strain (unspecified background) to remove the neomycin cassettes. Truncated exon 16 is incorporated into the sAPPbeta-FLAG transcript, but exon 17 is not expressed. This line (commonly called ADF) has been crossed with C57BL/6 and maintained on a mixed background by the donating laboratory.
Allele Name | targeted mutation 1, Thomas C Sudhof |
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Allele Type | Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence) |
Allele Synonym(s) | ADF |
Gene Symbol and Name | App, amyloid beta (A4) precursor protein |
Gene Synonym(s) | |
Promoter | App, amyloid beta (A4) precursor protein, mouse, laboratory |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 16 |
Molecular Note | The targeting vector incorporates a wildtype mouse App exon 16 (truncated after residue KM) followed by a FLAG tag (2 repeats), a stop codon, a poly A signal region from the human growth hormone gene, and a pair of neomycin resistance cassettes which are flanked by a pair of FRT sites. All of these elements are flanked by a single pair or loxP sites. This construct is followed by an additional copy of exon 16 carrying the Swedish mutation and modified version of exon 17 carrying the London mutation plus the Dutch mutation. Mice were crossed with an FLP strain (unspecified background) to remove the neomycin cassettes. Truncated exon 16 is incorporated into the sAPPbeta-FLAG transcript, but exon 17 is not expressed. |
Mutations Made By | Dr. Thomas Sudhof, Stanford University School of Medicine |
When maintained as a live colony, homozygotes or heterozygotes may be bred.
When using the STOCK Apptm1Sud/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008390 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for App<tm1Sud> |
Frozen Mouse Embryo | STOCK App<tm1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK App<tm1Sud>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | STOCK App<tm1Sud>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | STOCK App<tm1Sud>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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