Overview

These targeted mutant (knock-in line ADF) mice carry an insertion incorporating a truncated exon 16 of the mouse amyloid beta (A4) precursor protein (App) gene as well as the Swedish mutant exon 16 and London/Dutch mutations of exon 17. Truncated exon 16 is incorporated into the sAPPbeta-FLAG transcript, but exon 17 is not expressed. No overt phenotype has been observed. Homozygotes are viable and fertile. This strain may be useful in studies of Alzheimer's disease.

Donating Investigator

Dr. Thomas C. Sudhof, Stanford University School of Medicine

Genetic overview

Genetic Background	Generation

App^tm1Sud

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence)	App	amyloid beta (A4) precursor protein

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Research Applications

Neurobiology Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

These targeted mutant (knock-in line ADF) mice carry an insertion incorporating a truncated exon 16 of the mouse amyloid beta (A4) precursor protein (App) gene as well as the Swedish mutant exon 16 and London/Dutch mutations of exon 17. Truncated exon 16 is incorporated into the sAPPbeta-FLAG transcript, but exon 17 is not expressed. No overt phenotype has been observed. Homozygotes are viable and fertile. This strain may be useful in studies of Alzheimer's disease.

Development

The first portion of the targeting vector incorporates a wildtype mouse App exon 16 (truncated after residue KM) followed by a FLAG tag (2 repeats), a stop codon, a poly A signal region from the human growth hormone gene, and a pair of neomycin resistance cassettes which are flanked by a pair of FRT sites. All of these elements are flanked by a single pair or loxP sites. This construct is followed by an additional copy of exon 16 carrying the Swedish mutation and modified version of exon 17 carrying the London mutation plus the Dutch mutation.

This vector was introduced to (129X1/SvJ x 129S1/Sv)F1- Kitl⁺-derived R1 embryonic stem (ES) cells. Resultant mice were crossed with an FLP strain (unspecified background) to remove the neomycin cassettes. Truncated exon 16 is incorporated into the sAPPbeta-FLAG transcript, but exon 17 is not expressed. This line (commonly called ADF) has been crossed with C57BL/6 and maintained on a mixed background by the donating laboratory.

Selected References

Sudhof TC. 2008. Generation of an App knock-in allele MGI Direct Data SubmissionMGI: J:133653

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Genetics

App^tm1Sud

Allele Symbol: App^tm1Sud

Allele Name	targeted mutation 1, Thomas C Sudhof
Allele Type	Targeted (Conditional ready (e.g. floxed), Inserted expressed sequence)
Allele Synonym(s)	App^tm1Sud; targeted mutation 1, Thomas C Sudhof
Gene Symbol and Name	App, amyloid beta (A4) precursor protein
Gene Synonym(s)	Cvap; E030013M08Rik; betaAPP; Alzheimer disease amyloid beta protein; E030013M08Rik; Adap; ABETA; APPI; ABPP; AD1; AL024401; CTFgamma; PN-II; PN2; cerebrovascular amyloid peptide; CVAP; appican; Adap; protease nexin II; AAA; expressed sequence AL024401; RIKEN cDNA E030013M08 gene; Abeta; Cvap; preA4
Promoter	App, amyloid beta (A4) precursor protein, mouse, laboratory
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	16
Molecular Note	The targeting vector incorporates a wildtype mouse App exon 16 (truncated after residue KM) followed by a FLAG tag (2 repeats), a stop codon, a poly A signal region from the human growth hormone gene, and a pair of neomycin resistance cassettes which are flanked by a pair of FRT sites. All of these elements are flanked by a single pair or loxP sites. This construct is followed by an additional copy of exon 16 carrying the Swedish mutation and modified version of exon 17 carrying the London mutation plus the Dutch mutation. Mice were crossed with an FLP strain (unspecified background) to remove the neomycin cassettes. Truncated exon 16 is incorporated into the sAPPbeta-FLAG transcript, but exon 17 is not expressed.
Mutations Made By	Dr. Thomas Sudhof, Stanford University School of Medicine

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Alzheimer's Disease

Mammalian Phenotype Terms by Genotype

The following phenotype relates to a compound genotype created using this strain

Genotype: App^tm1Sud/App^tm1Sud
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

no abnormal phenotype detected
- Normal - mice display no overt abnormal phenotype, and are viable and fertile

References

Sudhof TC. 2008. Generation of an App knock-in allele MGI Direct Data SubmissionMGI: J:133653

Additional - App^tm1Sud related

Technical Support

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Genotyping Protocols
- Genotyping resources and troubleshooting
Breeding Considerations

When maintained as a live colony, homozygotes or heterozygotes may be bred.
- Additional Breeding and Husbandry Support
Citation
When using the STOCK App^tm1Sud/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008390 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

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Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	Heterozygous for App<tm1Sud>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Selected References

Apptm1Sud

Allele Symbol: Apptm1Sud

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Apptm1Sud/Apptm1Sudinvolves: 129S1/Sv * 129X1/SvJ * C57BL/6

normal phenotype

References

Additional - Apptm1Sud related

Genotyping Protocols

Breeding Considerations

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

App^tm1Sud

Allele Symbol: App^tm1Sud

Genotype: App^tm1Sud/App^tm1Sud
involves: 129S1/Sv * 129X1/SvJ * C57BL/6

Additional - App^tm1Sud related