These toll-like receptor 7 (Tlr7) targeted mutation mice may be useful in studies of viral immunity. Homozygotes demonstrate reduced responses to in vivo infection with vesicular stomatitis virus. The lacZ reporter introduced under the gene's promoter shows that within mesenteric lymph nodes, expression is normally confined to the perifollicular regions.
The Jackson Laboratory Repository has two Tlr7 knock-in alleles available: the Tlr7 knock-in/knock-out allele with lacZ replacing exon 3 (Tlr7tm1Flv; Stock No. 008380) and the TLR7KI-tdTom knock-in allele with an IRES-tdTomato at the 3' end (Tlr7tm1.1Gbrt; Stock No. 031827).
Dr. Richard A. Flavell, Yale University School of Medicine
These toll-like receptor 7 targeted mutation mice may be useful in studies of viral immunity. Homozygotes demonstrate reduced responses to in vivo infection with vesicular stomatitis virus. This emphasizes the roll of the gene in viral recognition by plasmacytoid dendritic cells and B cells which activate costimulatory molecules and produce cytokines. No RNA expression is detected in bone marrow-derived macrophages by Northern blot analysis. The lacZ reporter introduced under the gene's promoter shows that within mesenteric lymph nodes, expression is normally confined to the perifollicular regions. Homozygotes are viable, fertile, have no obvious developmental problems, but are poor breeders for unknown reasons.
A segment of exon 3 was replaced by a lacZ gene and loxP-flanked neomycin resistance cassette. The targeting vector was introduced to 129S1/Sv-p+ Tyr+ Kitl+-derived CJ7 embryonic stem (ES) cells. This line was backcrossed ten times to C57BL/6Ncr by the donating laboratory (see SNP note below). Upon arrival at The Jackson Laboratory, this strain was crossed one time to C57BL/6J.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
|Expressed Gene||lacZ, beta-galactosidase, E. coli|
|Site of Expression|
|Allele Name||targeted mutation 1, Richard A Flavell|
|Allele Type||Targeted (Reporter, Null/Knockout)|
|Allele Synonym(s)||Tlr7tm1Flv; targeted mutation 1, Richard A Flavell|
|Gene Symbol and Name||Tlr7, toll-like receptor 7|
|Gene Synonym(s)||TLR7-like; RGD1563357|
|Expressed Gene||lacZ, beta-galactosidase, E. coli|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A cassette containing lacZ and floxed neo replaced a portion of exon 3. Northern blot analysis of mutant bone marrow-derived macrophages indicated no mRNA was present. Beta galactose staining showed expression of lacZ in mutant lymph nodes.|
|Mutations Made By|| |
Dr. Richard Flavell, Yale University School of Medicine
When maintained as a live colony, homozygotes may be bred with hemizygotes (X linked).
When using the TLR7- mouse strain in a publication, please cite the originating article(s) and include JAX stock #008380 in your Materials and Methods section.
|X linked - Heterozygous females and Wild-type Males for Tlr7<tm1Flv>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
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