These toll-like receptor 7 (Tlr7) targeted mutation mice may be useful in studies of viral immunity. Homozygotes demonstrate reduced responses to in vivo infection with vesicular stomatitis virus. The lacZ reporter introduced under the gene's promoter shows that within mesenteric lymph nodes, expression is normally confined to the perifollicular regions.
The Jackson Laboratory Repository has two Tlr7 knock-in alleles available: the Tlr7 knock-in/knock-out allele with lacZ replacing exon 3 (Tlr7tm1Flv; Stock No. 008380) and the TLR7KI-tdTom knock-in allele with an IRES-tdTomato at the 3' end (Tlr7tm1.1Gbrt; Stock No. 031827).
Dr. Richard A. Flavell, Yale University School of Medicine
Genetic Background | Generation |
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N10+N1F9
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Reporter, Null/Knockout) | Tlr7 | toll-like receptor 7 |
These toll-like receptor 7 targeted mutation mice may be useful in studies of viral immunity. Homozygotes demonstrate reduced responses to in vivo infection with vesicular stomatitis virus. This emphasizes the roll of the gene in viral recognition by plasmacytoid dendritic cells and B cells which activate costimulatory molecules and produce cytokines. No RNA expression is detected in bone marrow-derived macrophages by Northern blot analysis. The lacZ reporter introduced under the gene's promoter shows that within mesenteric lymph nodes, expression is normally confined to the perifollicular regions. Homozygotes are viable, fertile, have no obvious developmental problems, but are poor breeders for unknown reasons.
A segment of exon 3 was replaced by a lacZ gene and loxP-flanked neomycin resistance cassette. The targeting vector was introduced to 129S1/Sv-p+ Tyr+ Kitl+-derived CJ7 embryonic stem (ES) cells. This line was backcrossed ten times to C57BL/6Ncr by the donating laboratory (see SNP note below). Upon arrival at The Jackson Laboratory, this strain was crossed one time to C57BL/6J.
A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
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Site of Expression |
Allele Name | targeted mutation 1, Richard A Flavell |
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Allele Type | Targeted (Reporter, Null/Knockout) |
Allele Synonym(s) | Tlr7- |
Gene Symbol and Name | Tlr7, toll-like receptor 7 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Strain of Origin | 129S1/Sv-Oca2+ Tyr+ Kitl+ |
Chromosome | X |
Molecular Note | A cassette containing lacZ and floxed neo replaced a portion of exon 3. Northern blot analysis of mutant bone marrow-derived macrophages indicated no mRNA was present. Beta galactose staining showed expression of lacZ in mutant lymph nodes. |
Mutations Made By | Dr. Richard Flavell, Yale University School of Medicine |
When maintained as a live colony, homozygotes may be bred with hemizygotes (X linked).
When using the TLR7- mouse strain in a publication, please cite the originating article(s) and include JAX stock #008380 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
X linked - Heterozygous females and Wild-type Males for Tlr7<tm1Flv> |
Frozen Mouse Embryo | B6.129S1-Tlr7<tm1Flv>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Tlr7<tm1Flv>/J | $2595.00 |
Frozen Mouse Embryo | B6.129S1-Tlr7<tm1Flv>/J | $3373.50 |
Frozen Mouse Embryo | B6.129S1-Tlr7<tm1Flv>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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