Overview

Also Known As: TLR7^-

These toll-like receptor 7 (Tlr7) targeted mutation mice may be useful in studies of viral immunity. Homozygotes demonstrate reduced responses to in vivo infection with vesicular stomatitis virus. The lacZ reporter introduced under the gene's promoter shows that within mesenteric lymph nodes, expression is normally confined to the perifollicular regions.

The Jackson Laboratory Repository has two Tlr7 knock-in alleles available: the Tlr7 knock-in/knock-out allele with lacZ replacing exon 3 (Tlr7^tm1Flv; Stock No. 008380) and the TLR7KI-tdTom knock-in allele with an IRES-tdTomato at the 3' end (Tlr7^tm1.1Gbrt; Stock No. 031827).

Donating Investigator

Dr. Richard A. Flavell, Yale University School of Medicine

Genetic overview

Genetic Background	Generation
	N10+N1F9 (2019-06-17 00:00:00)

Tlr7^tm1Flv

Allele Type	Gene Symbol	Gene Name
Targeted (Reporter, Null/Knockout)	Tlr7	toll-like receptor 7

View Genetics

Research Applications

Immunology, Inflammation and Autoimmunity Research
Virology Research

View All Research Applications

Base Price

Starting at:

$0.00 Domestic price for male

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Details

Detailed Description

These toll-like receptor 7 targeted mutation mice may be useful in studies of viral immunity. Homozygotes demonstrate reduced responses to in vivo infection with vesicular stomatitis virus. This emphasizes the roll of the gene in viral recognition by plasmacytoid dendritic cells and B cells which activate costimulatory molecules and produce cytokines. No RNA expression is detected in bone marrow-derived macrophages by Northern blot analysis. The lacZ reporter introduced under the gene's promoter shows that within mesenteric lymph nodes, expression is normally confined to the perifollicular regions. Homozygotes are viable, fertile, have no obvious developmental problems, but are poor breeders for unknown reasons.

Development

A segment of exon 3 was replaced by a lacZ gene and loxP-flanked neomycin resistance cassette. The targeting vector was introduced to 129S1/Sv-p⁺ Tyr⁺ Kitl⁺-derived CJ7 embryonic stem (ES) cells. This line was backcrossed ten times to C57BL/6Ncr by the donating laboratory (see SNP note below). Upon arrival at The Jackson Laboratory, this strain was crossed one time to C57BL/6J.

A 32 SNP (single nucleotide polymorphism) panel analysis, with 27 markers covering all 19 chromosomes and the X chromosome, as well as 5 markers that distinguish between the C57BL/6J and C57BL/6N substrains, was performed on the rederived living colony at The Jackson Laboratory Repository. While the 27 markers throughout the genome suggested a C57BL/6 genetic background, all 5 markers that determine C57BL/6J from C57BL/6N were found to be segregating. These data suggest the mice sent to The Jackson Laboratory Repository were on a C57BL/6N genetic background.

Expression Data

Expressed Gene	lacZ, beta-galactosidase, E. coli
Site of Expression

Control Suggestions

Approximate Controls

005304 C57BL/6NJ

Additional Information

Considerations for Choosing Controls

Selected References

Lund JM; Alexopoulou L; Sato A; Karow M; Adams NC; Gale NW; Iwasaki A; Flavell RA. 2004. Recognition of single-stranded RNA viruses by Toll-like receptor 7. Proc Natl Acad Sci U S A 101(15):5598-603PubMed: 15034168 MGI: J:89257

View All References

Genetics

Tlr7^tm1Flv

Allele Symbol: Tlr7^tm1Flv

Allele Name	targeted mutation 1, Richard A Flavell
Allele Type	Targeted (Reporter, Null/Knockout)
Allele Synonym(s)	Tlr7^tm1Flv; targeted mutation 1, Richard A Flavell
Gene Symbol and Name	Tlr7, toll-like receptor 7
Gene Synonym(s)	TLR7-like; RGD1563357
Expressed Gene	lacZ, beta-galactosidase, E. coli
Strain of Origin	129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺
Chromosome	X
Molecular Note	A cassette containing lacZ and floxed neo replaced a portion of exon 3. Northern blot analysis of mutant bone marrow-derived macrophages indicated no mRNA was present. Beta galactose staining showed expression of lacZ in mutant lymph nodes.
Mutations Made By	Dr. Richard Flavell, Yale University School of Medicine

Disease/Phenotype

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Immunology, Inflammation and Autoimmunity Research
- CD Antigens, Antigen Receptors, and Histocompatibility Markers
  - Tlr deficiency
  - genes regulating susceptibility to infectious disease and endotoxin
- Immunodeficiency
  - Tlr deficiency
- Inflammation
  - Tlr deficiency
Virology Research

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Tlr7^tm1Flv/Tlr7^tm1Flv
involves: 129S1/Sv

homeostasis/metabolism phenotype

decreased circulating interferon-alpha level
- while IFNalpha secretion in response to infection with vesicular stomatitis virus (a ssRNA virus) was similar to controls, serum levels of IFNalpha were reduced after infection relative to controls

immune system phenotype

decreased circulating interferon-alpha level
- while IFNalpha secretion in response to infection with vesicular stomatitis virus (a ssRNA virus) was similar to controls, serum levels of IFNalpha were reduced after infection relative to controls

The following phenotype relates to a compound genotype created using this strain

Genotype: Tlr7^tm1Flv/Tlr7^tm1Flv
B6.129S1-Tlr7

hematopoietic system phenotype

decreased IgG level
- in the serum and as glomerular deposition

decreased IgG1 level

decreased IgG2a level

decreased IgM level
- in the serum and as glomerular deposition

immune system phenotype

decreased anti-insulin autoantibody level

decreased IgG level
- in the serum and as glomerular deposition

decreased IgG1 level

decreased IgG2a level

decreased IgM level
- in the serum and as glomerular deposition

Genotype: Tlr7^tm1Flv/Y
B6.129S1-Tlr7

cellular phenotype

decreased splenocyte proliferation
- absence of the splenocyte proliferation response to imiquimod

hematopoietic system phenotype

decreased splenocyte proliferation
- absence of the splenocyte proliferation response to imiquimod

increased marginal zone B cell number
- slight increase

immune system phenotype

decreased splenocyte proliferation
- absence of the splenocyte proliferation response to imiquimod

increased marginal zone B cell number
- slight increase

References

Lund JM; Alexopoulou L; Sato A; Karow M; Adams NC; Gale NW; Iwasaki A; Flavell RA. 2004. Recognition of single-stranded RNA viruses by Toll-like receptor 7. Proc Natl Acad Sci U S A 101(15):5598-603PubMed: 15034168 MGI: J:89257

Additional References

Zhang ZJ; Guo JS; Li SS; Wu XB; Cao DL; Jiang BC; Jing PB; Bai XQ; Li CH; Wu ZH; Lu Y; Gao YJ. 2018. TLR8 and its endogenous ligand miR-21 contribute to neuropathic pain in murine DRG. J Exp Med 215(12):3019-3037PubMed: 30455267 MGI: J:269886
Liu CL; Santos MM; Fernandes C; Liao M; Iamarene K; Zhang JY; Sukhova GK; Shi GP. 2017. Toll-like receptor 7 deficiency protects apolipoprotein E-deficient mice from diet-induced atherosclerosis. Sci Rep 7(1):847PubMed: 28405010 MGI: J:271858

Additional - Tlr7^tm1Flv related

Technical Support

Contact Technical Support

Genotyping Protocols
- Standard PCR: Tlr7^tm1Flv
- Genotyping resources and troubleshooting
Breeding Considerations

When maintained as a live colony, homozygotes may be bred with hemizygotes (X linked).
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Hemizygote
Citation
When using the TLR7^- mouse strain in a publication, please cite the originating article(s) and include JAX stock #008380 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX11 (Maximum)

Pricing & Availability

Available

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service	Genotype	Price
	X linked - Heterozygous females and Wild-type Males for Tlr7<tm1Flv>

We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.

Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.

Related Products and Services

Frozen Mouse Embryo

$2,595.00 per straw or vial

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.

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General Terms and Conditions

Questions about Terms of Use

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Licensing Information

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Also Known As: TLR7-

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Approximate Controls

Additional Information

Selected References

Tlr7tm1Flv

Allele Symbol: Tlr7tm1Flv

Disease Terms

Research Areas By Genotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Tlr7tm1Flv/Tlr7tm1Flvinvolves: 129S1/Sv

homeostasis/metabolism phenotype

immune system phenotype

Genotype: Tlr7tm1Flv/Tlr7tm1FlvB6.129S1-Tlr7

hematopoietic system phenotype

immune system phenotype

Genotype: Tlr7tm1Flv/YB6.129S1-Tlr7

cellular phenotype

hematopoietic system phenotype

immune system phenotype

References

Additional References

Additional - Tlr7tm1Flv related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms of Use

Additional Use Restrictions Apply

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Also Known As: TLR7^-

Tlr7^tm1Flv

Allele Symbol: Tlr7^tm1Flv

Genotype: Tlr7^tm1Flv/Tlr7^tm1Flv
involves: 129S1/Sv

Genotype: Tlr7^tm1Flv/Tlr7^tm1Flv
B6.129S1-Tlr7

Genotype: Tlr7^tm1Flv/Y
B6.129S1-Tlr7

Additional - Tlr7^tm1Flv related