These LiasLow hypomorph mice under express lipoic acid synthetase. These mice may be suitable for use in studies related to oxidative stress and the role of antioxidants in disease.
Read More +Genetic Background | Generation |
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Allele Type | Gene Symbol | Gene Name |
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Targeted (Hypomorph) | Lias | lipoic acid synthetase |
The targeted Lias gene encodes a lipoic acid synthetase that is critical in synthesis of alpha-lipoic acid. These LiasHigh mice carry a hypomorph allele of Lias gene, with cFos gene 3'-UTR sequence directing expression of the endogenous Lias.
Homozygous LiasLow mice have unstabilized Lias transcripts resulting in reduced expression and LIAS protein levels approximately 25% compared to levels in wildtype mice, as assessed by Western blot analysis of kidney tissue.
When crossed with C57BL/6-Ins2Akita/J mice (Stock No. 003548) the resulting double mutant mice exhibit more severe diabetic nephropathy features and higher oxidative stress when compared to the single mutant C57BL/6-Ins2Akita/J mice.
A targeting vector designed by Dr. Nobuyo Maeda (University of North Carolina, Chapel Hill) containing
loxP site flanked bovine growth hormone gene 3’-UTR sequence and a NEO cassette, and 3’-UTR sequence of the cFos gene was inserted into the 3’-UTR of the Lias gene. The construct was electroporated into C57BL/6N derived Chemicon B/6N embryonic stem (ES) cells at The Jackson Laboratory. Correctly targeted ES cells were injected into albino C57BL/6J blastocysts. The resulting chimeric mice were crossed to C57BL/6J (Stock No. 000664). LiasHigh (LiasH) heterozygotes were crossed to B6.FVB-Tg(EIIa-cre)C5379Lmgd/J (Stock No. 003724) mice to excise the floxed bovine growth hormone gene 3’-UTR sequence and a NEO cassette. The resulting LiasLow mice were bred to C57BL/6J to remove the transgene. Sperm was cryopreserved.
Allele Name | targeted mutation 2.1, Nobuyo Maeda |
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Allele Type | Targeted (Hypomorph) |
Allele Synonym(s) | LiasLow |
Gene Symbol and Name | Lias, lipoic acid synthetase |
Gene Synonym(s) | |
Strain of Origin | C57BL/6N |
Chromosome | 5 |
General Note | ES cell line - Chemicon B/6N |
Molecular Note | The targeting vector consists of a loxP site, the 3' UTR sequence of the bovine growth hormone gene, a neomycin cassette and a loxP site followed by the 3'-UTR sequence of the cFos gene. Cre-mediated recombination removed the bovine growth hormone gene and neo cassette, allowing cFos to drive expression of the endogenous gene. Expression is 25% of the endogenous gene expression. |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the LiasLow mouse strain in a publication, please cite the originating article(s) and include JAX stock #008350 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Heterozygous for Lias<tm2.1Mae> |
Frozen Mouse Embryo | B6(FVB)-Lias<tm2.1Mae>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(FVB)-Lias<tm2.1Mae>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6(FVB)-Lias<tm2.1Mae>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6(FVB)-Lias<tm2.1Mae>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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