These T1-147 transgenic mice (harboring the E1-T147 transgene) develop metastatic pancreatic acinar cell cancer as a result of expression of the 1-147 amino acid fragment of simian virus 40 large T antigen (TAg) directed to pancreatic acinar cells by the rat elastase 1 (Ela1 or E1) upstream control region.
Todd D. Schell, Penn State Univ. College of Medicine
Hemizygous T1-147 transgenic mice (harboring the E1-T147 transgene) are viable and fertile, with high-level expression of an N-terminal, truncated (1-147 amino acid), and mutant (does not express the small t antigen) form of simian virus 40 large T antigen (TAg) directed to pancreatic acinar cells by the rat elastase 1 (Ela1 or E1) upstream control region. Hemizygous T1-147 transgenic mice exhibit pancreatic dysplasia in the embryonic pancreas, with cancer progression after birth to primary pancreatic acinar cell tumor formation (100% penetrance). These mice have microadenomas and acinar cell carcinomas present as early as 10 weeks of age, and become moribund or show clear evidence of abdominal distention around 20-30 weeks of age. T1-147 transgenic mice with large tumors develop metastatic disease to the liver and mesenteric lymph nodes. The donating investigator reports that the cancer phenotype of these T1-147 transgenic mice is identical to that of their similar T1-127 transgenic mice. These T1-147 transgenic mice may be useful in studying pancreatic acinar cell cancer and metastasis.
The E1-T147 transgene was designed with a 4.3 kb rat elastase 1 (Ela1 or E1) gene upstream control region, LTR stabilizer, and SV40 late 16S splice site all upstream of the simian virus 40 large T antigen (TAg) coding sequence for amino acids 1-147 (with stop codon introduced at amino acid 148) and polyA sequence. This transgene was microinjected into pronuclei of B6D2F1/J x C57BL/6 embryos, and the resulting transgenic offspring were bred to C57BL/6J mice. The T1-147 transgenic mice (founder line 289) mice were established, and subsequently backcrossed to C57BL/6 inbred mice for at least 20 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice were bred with C57BL/6J (Stock No. 000664) to establish the colony.
|Expressed Gene||TAg, SV40 large T-antigen, SV40|
|Site of Expression|
|Allele Name||transgene insertion 289, Mary J Tevethia|
|Allele Type||Transgenic (Inserted expressed sequence)|
|Gene Symbol and Name||Tg(Ela1-TAg*)289Mjt, transgene insertion 289, Mary J Tevethia|
|Promoter||Cela1, chymotrypsin-like elastase family, member 1, rat|
|Expressed Gene||TAg, SV40 large T-antigen, SV40|
|Strain of Origin||(B6D2F1/J x C57BL/6)|
|Molecular Note||The E1-T147 transgene was designed with a 4.3 kb rat elastase 1 (Ela1 or E1) gene upstream control region, LTR stabilizer, and SV40 late 16S splice site all upstream of the simian virus 40 large T antigen (TAg) coding sequence for amino acids 1-147 (with stop codon introduced at amino acid 148) and polyA sequence. The T1-147 transgenic mice (founder line 289) mice were established.|
|Mutations Made By|| |
Mary Tevethia, Penn State Univ. College of Medicine
When maintaining a live colony, hemizygous mice may be bred with noncarrier littermates or with C57BL/6J inbred mice. The donating investigator breeds hemizygous males with noncarrier females, and reports decreased litter sizes may result from breeding to homozygosity.
When using the B6.Cg-Tg(Ela1-TAg*)289Mjt/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008247 in your Materials and Methods section.
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