These E-selectin mutant mice may be useful in studying inflammation, leukocyte rolling, leukocyte-endothelial adhesion, angiogenesis, and cancer.
David S Milstone, Brigham and Women's Hospital
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Sele | selectin, endothelial cell |
Mice homozygous for this E-selectin mutant allele (E-/-) are viable and fertile with normal circulating leukocyte and platelet profiles. While several transcripts are generated from the mutant locus (due to transcription from the endogenous promoter and/or bidirectional transcription initiated from the pgk promoter in the neo-resistance cassette), these frame-shifted transcripts are non-functional with several predicted stop codons. In contrast to wildtype mice, no protein product is detected in several tissues isolated from LPS-injected homozygous mice. Homozygous mice exhibit abnormal responses to inflammatory stimuli. E-selectin deficiency results in endostatin unresponsiveness (as shown in corneal angiogenesis (mixed B6;129 genetic background) and aortic explant (C57BL/6 congenic background) experiments). These E-selectin mutant mice may be useful in studying inflammation, leukocyte rolling, leukocyte-endothelial adhesion, angiogenesis, and cancer.
Of note, E-selectin mutant mice are also be available on a C57BL/6J congenic background (Stock No. 008236), a BALB/cJ congenic background (Stock No. 008237), on a mixed genetic background (Stock No. 002915), and along with other selectin mutations (Stock No. 002916, Stock No. 003806, and Stock No. 003807).
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype of these E-selectin mutant mice could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to replace exons 1-3 of the targeted gene with a reverse-oriented PGK-neo cassette. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric males were bred with C57BL/6J to generate mutant mice. Next, mutant mice were backcrossed to 129S6/SvEvTac inbred mice for 11 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed to the genetic background during the backcross.
Allele Name | targeted mutation 1, David Milstone |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | E-; E-selectin- |
Gene Symbol and Name | Sele, selectin, endothelial cell |
Gene Synonym(s) | |
Strain of Origin | 129S4/SvJae |
Chromosome | 1 |
Molecular Note | The neomycin resistance gene was substituted in reverse transcriptional orientation for sequence spanning from exon 1 to exon 3. Northern blot confirmed absence of transcript in mutant kidney, lung and heart samples. |
Mutations Made By | David Milstone, Brigham and Women's Hospital |
When maintaining a live colony, homozygous mice may be bred.
When using the 129S-Seletm1Dmil/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008238 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Homozygous for Sele<tm1Dmil>, 1 pair minimum |
Frozen Mouse Embryo | 129S-Sele<tm1Dmil>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129S-Sele<tm1Dmil>/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | 129S-Sele<tm1Dmil>/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | 129S-Sele<tm1Dmil>/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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