These GATA binding protein 4 conditional mice may be useful in generating conditional mutations for studying GATA4 function during organogenesis (such as cardiogenesis) or in adult mice.
Stephen Duncan, Medical College of Wisconsin
Mice homozygous for this Gata4loxP conditional allele are viable and fertile, with loxP sites flanking exons 3-5 of the targeted gene. When bred to mice that express Cre recombinase, the resulting offspring will have this region (coding for both zinc finger DNA-binding domains and the nuclear localization signal essential for GATA4 function) deleted in the cre-expressing tissue(s). These GATA binding protein 4 conditional mice may be useful in generating conditional mutations for studying GATA4 function during organogenesis (such as cardiogenesis) or in adult mice.
For example, when crossed to a strain expressing Cre recombinase in cardiac myocytes (see Stock No. 009074), this mutant mouse strain may be useful in studies of cardiac hypertrophy, stress-compensation and myocyte viability.
When bred to a strain expressing Cre recombinase in heart muscle (see Stock No. 010581 for example), this mutant mouse strain may be useful in studies of cardiac hypertrophy.
A targeting vector was designed to place a loxP-flanked thymidine kinase/neomycin phosphotransferase expression (tk/neo) cassette upstream of exon 3, and a third loxP site just downstream of exon 5 of the targeted gene. The targeting construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre expressing plasmid to remove the selection cassette (generating the Gata4loxP allele; with one loxP site upstream of exon 3 and one loxP site downstream of exon 5). These ES cells were combined with CD1 morula to generate chimeric animals, which were then bred with CD1 mice. These Gata4loxP mice were maintained on a mixed genetic background prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to 129S1/SvImJ for at least one generation to establish the colony.
|Allele Name||targeted mutation 1.1, Stephen A Duncan|
|Allele Type||Targeted (Conditional ready (e.g. floxed), No functional change)|
|Gene Symbol and Name||Gata4, GATA binding protein 4|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||A targeting vector was designed to place a loxP-flanked thymidine kinase/neomycin phosphotransferase expression (tk/neo) cassette upstream of exon 3, and a third loxP site just downstream of exon 5 of the targeted gene. Correctly targeted ES cells were transiently transfected with a cre expressing plasmid to remove the selection cassette (generating the Gata4-loxP allele; with one loxP site upstream of exon 3 and one loxP site downstream of exon 5). (details provided by SA Duncan, personal communication.)|
|Mutations Made By|| |
Stephen Duncan, Medical College of Wisconsin
When maintaining a live colony, homozygous mice may be bred together.
When using the STOCK Gata4tm1.1Sad/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008194 in your Materials and Methods section.
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