Mice homozygous for this S23A point mutation are viable and fertile. Cells from the mice, including thymocytes and neurons in the cerebellum, exhibit defective apoptosis in response to DNA damage and exhibit partially impaired TRP53 (p53) stabilization. Mice develop increased lymphomas (especially B-cell lineage) and other tumors between one and two years of age.
Dr. Tyler Jacks, Massachusetts Institute of Technology
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Not Specified) | Trp53 | transformation related protein 53 |
Mice homozygous for this S23A point mutation are viable and fertile. Cells from the mice, including thymocytes and neurons in the cerebellum, exhibit defective apoptosis in response to DNA damage and exhibit partially impaired TRP53 (p53) stabilization. Mice develop increased lymphomas (especially B-cell lineage) and other tumors between one and two years of age.
A targeting vector was designed to introduce a Ser23Ala point mutation to exon 2 of the gene and place a loxP-flanked puromycin resistance cassette in reverse orientation in intron 1. The construct was electroporated into 129S4/SvJae-derived J1 embryonic stem (ES) cells. Cre recombinase was introduced to targeted ES cells by transient transfection to delete the puromycin cassette. The strain has been maintained on a mixed 129S4/SvJae and C57BL/6 by the donating laboratory.
Allele Name | targeted mutation 4, Tyler Jacks |
---|---|
Allele Type | Targeted (Not Specified) |
Allele Synonym(s) | S23A |
Gene Symbol and Name | Trp53, transformation related protein 53 |
Gene Synonym(s) | |
Promoter | Trp53, transformation related protein 53, mouse, laboratory |
Strain of Origin | 129S4/SvJae |
Chromosome | 11 |
Molecular Note | A serine to alanine mutation was created at position 23 in exon 2 (S23A). Transient cre recombinase expression excised a floxed puromycin resistance cassette that was introduced with the mutation. RT-PCR of mutant MEFs confirmed correct targeting. |
Mutations Made By | Dr. Tyler Jacks, Massachusetts Institute of Technology |
When maintained as a live colony, heterozygotes or homozygotes may be bred.
When using the B6;129S4-Trp53tm4Tyj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008181 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Trp53<tm4Tyj> |
Frozen Mouse Embryo | B6;129S4-Trp53<tm4Tyj>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Trp53<tm4Tyj>/J | $2595.00 |
Frozen Mouse Embryo | B6;129S4-Trp53<tm4Tyj>/J | $3373.50 |
Frozen Mouse Embryo | B6;129S4-Trp53<tm4Tyj>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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