NOD.L-(Csf2-D11Mit339)/McdfJ

Stock number: 008056
Product name: NOD.Lc11e and DR3e
Strain synonyms: DR3e, NOD.Lc11e
Research areas: Diabetes and Obesity Research, Immunology, Inflammation and Autoimmunity Research

Description

Stock No.'s 008053, 008054, 008055, 008056, 008057, 008058, 008059 and 008060, are the result of crossing segments of C57L/J derived Chromosome 11 into the NOD/Bdc background. These strains experience significantly reduced diabetes and are useful for identifying candidate genes within the Idd4 subloci.

Detailed description

This NOD congenic strain, commonly referred to as NOD.Lc11e or DR3e carries Chromosome 11 alleles, D11Mit164 (56Mb) through D11Mit339 (63Mb) derived from C57L/J including a small part of Idd4 that includes candidate gene Csf2. Diabetes incidence in this strain is reported to be about 53% in females compared to 70% in female NOD control mice. (Davoodi-Semiromi et al 2007).

There appear to be multiple diabetes resistant regions (Idd4) on Chromosome 11, therefore, this strain is useful to identify candidate genes associated with diabetes resistance and to positionally clone the Idd4 resistant loci.

Development

This NOD congenic stock (NOD.Lc11e, DR3e) contains a genomic segment from C57L/J introgressed into Chr 11. Seven additional stocks (Stock No's 008054, 008055, 008056, 008057, 008058, 008059, and 008060) were developed from Stock No. 008053 through backcrossing to NOD once and selective breeding. This model, Stock No. 008056, contains a C57L/J congenic interval D11Mit164 (56Mb) through D11Mit339 (63Mb) containing C57L/J derived Csf2 and NOD/ShiLt derived Stat5a/Stat5b/Stat3 alleles. In 2008, the Type 1 Diabetes Resource received this stock at N6F10.

Allele

MGI accession ID: MGI:96406
Marker symbol: Idd4
Marker name: insulin dependent diabetes susceptibility 4
Marker synonyms: Idd-4
Chromosome: 11

Allele

MGI accession ID: MGI:1339752
Marker symbol: Csf2
Marker name: colony stimulating factor 2 (granulocyte-macrophage)
Marker synonyms: CSF; Csfgm; GMCSF; Gm-csf; MGI-IGM
Chromosome: 11

Allele

MGI accession ID: MGI:102283
Marker symbol: D11Mit339
Marker name: DNA Segment, Chr 11, Massachusetts Institute of Technology 339
Chromosome: 11