B6;129P2-Adam2^tm1Dgm/J

Stock number: 008042
Research areas: Reproductive Biology Research, Research Tools

Description

These fertilin β (Adam2) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the cyritestin (Adam3)-deficient strain (see Stock No. 008043).

Detailed description

Mice homozygous for this fertilin β (Adam2) mutant allele are viable with no gross phenotypic abnormalities reported. Both precursor and processed fertilin β proteins were absent from spermatogenic cells and mature sperm isolated from homozygous males. While homozygous females are fertile with normal egg activation, homozygous males exhibit sperm deficiencies in sperm-egg membrane adhesion, sperm-egg fusion, migration from the uterus into the oviduct, and binding to the egg zona pellucida, rendering them infertile. These fertilin β (Adam2) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the cyritestin (Adam3)-deficient strain (see Stock No. 008043).

Development

A targeting vector was designed to replace exon 14 of the targeted gene with a PGK-neo cassette. The construct was electroporated into 129P2/OlaHsd-derived BK4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric males were bred with C57BL/6N to produce heterozygous mutant mice. The donating investigators maintained these mice by breeding homozygous females to heterozygous males for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.

Allele

Symbol: Adam2^tm1Dgm
Name: targeted mutation 1, Diana G Myles
MGI accession ID: MGI:1857764
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Adam2
Marker name: a disintegrin and metallopeptidase domain 2
Chromosome: 14
Strain of origin: 129P2/OlaHsd
Molecular note: A neomycin resistance cassette replaced a genomic fragment containing exon 14, encoding the predicted disintegrin domain active site. Western blot analysis did not detect the encoded protein in spermatogenic cells and sperm derived from homozygous mice.