These fertilin β (Adam2) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the cyritestin (Adam3)-deficient strain (see Stock No. 008043).
Paul Primakoff, University of California Davis
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Adam2 | a disintegrin and metallopeptidase domain 2 |
Mice homozygous for this fertilin β (Adam2) mutant allele are viable with no gross phenotypic abnormalities reported. Both precursor and processed fertilin β proteins were absent from spermatogenic cells and mature sperm isolated from homozygous males. While homozygous females are fertile with normal egg activation, homozygous males exhibit sperm deficiencies in sperm-egg membrane adhesion, sperm-egg fusion, migration from the uterus into the oviduct, and binding to the egg zona pellucida, rendering them infertile. These fertilin β (Adam2) mutant mice may be useful in reproductive biology studies; specifically to determine the role of ADAM (A Disintegrin And Metalloprotease) family proteins in sperm-egg interactions, fertilization, and spermatogenesis. These mice may also be useful in conjunction with other ADAM family mutant mice, including the cyritestin (Adam3)-deficient strain (see Stock No. 008043).
A targeting vector was designed to replace exon 14 of the targeted gene with a PGK-neo cassette. The construct was electroporated into 129P2/OlaHsd-derived BK4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into blastocysts and chimeric males were bred with C57BL/6N to produce heterozygous mutant mice. The donating investigators maintained these mice by breeding homozygous females to heterozygous males for many generations prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred to C57BL/6NJ (Stock No. 005304) for at least one generation to establish the colony.
Allele Name | targeted mutation 1, Diana G Myles |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | fertilin beta- |
Gene Symbol and Name | Adam2, a disintegrin and metallopeptidase domain 2 |
Gene Synonym(s) | |
Strain of Origin | 129P2/OlaHsd |
Chromosome | 14 |
Molecular Note | A neomycin resistance cassette replaced a genomic fragment containing exon 14, encoding the predicted disintegrin domain active site. Western blot analysis did not detect the encoded protein in spermatogenic cells and sperm derived from homozygous mice. |
Mutations Made By | Diana Myles, University of California Davis |
When maintaining a live colony, homozygous females are bred with heterozygous males. Homozygous males have severe sperm defects rendering them infertile.
When using the B6;129P2-Adam2tm1Dgm/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #008042 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or Wild-Type for Adam2<tm1Dgm> |
Frozen Mouse Embryo | B6;129P2-Adam2<tm1Dgm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Adam2<tm1Dgm>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;129P2-Adam2<tm1Dgm>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;129P2-Adam2<tm1Dgm>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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