Overview

Mice that are homozygous for this targeted mutation on the 129S genetic background exhibit diminished social interaction behavior, do not barber or trim whiskers of cagemates, show subordinance in social dominance tests, do not sleep huddled together and do not build nests. This mutant mouse strain may be useful in studies of social behavior, sensorimotor gating, and possibly psychiatric disorders.

Donating Investigator

Dr. Anthony Wynshaw-Boris, Case Western Reserve University, School of Medicine

Genetic overview

Genetic Background	Generation

Dvl1^tm1Awb

Allele Type	Gene Symbol	Gene Name
Targeted (Null/Knockout)	Dvl1	dishevelled segment polarity protein 1

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Research Applications

Neurobiology Research
Sensorineural Research

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Base Price

Starting at:

$2,854.50 Domestic price Cryo Recovery

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Details

Detailed Description

Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by Western blot analysis of MEF (mouse embryonic fibroblasts) derived from homozygotes. Homozygotes exhibit diminished social interaction behavior, do not barber or trim whiskers of cagemates, show subordinance in social dominance tests, do not sleep huddled together and do not build nests. Although mutants have an attenuated prepulse inhibition of acoustic and tactile startle (sensorimotor gating of the startle reflex), they do not exhibit any deficits in locomotor or cognitive function. This mutant mouse strain may be useful in studies of social behavior, sensorimotor gating, and possibly psychiatric disorders.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt part of exon 2 and exons 3 and 4, encoding amino acids 131-225. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice, and then backcrossed to C57BL/6J for 15 generations.

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Lijam N; Paylor R; McDonald MP; Crawley JN; Deng CX; Herrup K ; Stevens KE ; Maccaferri G ; McBain CJ ; Sussman DJ ; Wynshaw-Boris A. 1997. Social interaction and sensorimotor gating abnormalities in mice lacking Dvl1. Cell 90(5):895-905PubMed: 9298901 MGI: J:42758

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Genetics

Dvl1^tm1Awb

Allele Symbol: Dvl1^tm1Awb

Allele Name	targeted mutation 1, Anthony Wynshaw-Boris
Allele Type	Targeted (Null/Knockout)
Allele Synonym(s)	Dvl1-; Dvl1^del131-225
Gene Symbol and Name	Dvl1, dishevelled segment polarity protein 1
Gene Synonym(s)
Strain of Origin	129S6/SvEvTac
Chromosome	4
Molecular Note	A neomycin selection cassette replaced a genomic fragment containing part of exon 2 and exons 3 and 4, which encode amino acids 131-225. Western blot analysis on embryonic fibroblasts derived from homozygous mice confirmed that no detectable protein was produced from this allele.
Mutations Made By	Dr. Anthony Wynshaw-Boris, Case Western Reserve University, School of Medicine

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Behavioral and Learning Defects
Sensorineural Research

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Dvl1^tm1Awb/Dvl1^tm1Awb
involves: 129S6/SvEvTac

behavior/neurological phenotype

abnormal huddling behavior
- homozygotes sleep in scattered random patterns, unlike wild-type mice which generally sleep huddled together
- over a period of time, homozygotes tend to loosely huddle in various quandrants of the cage, whereas wild-type mice are more often observed huddled in the same quadrant

abnormal nest building behavior
- homozygotes build significantly shallower nests from nest building material relative to wild-type controls
- homozygotes tend to sleep on top of intact nestlet material whereas wild-type mice sleep in well-formed, fluffy nests
- Normal - however, abnormal nesting behavior is not due to differences in rectal temperatures

abnormal social/conspecific interaction
- significantly different between wild-type and mutant mice (41 vs 36 episodes, respectively)
- when housed separately as uniform genotypes, homozygotes display only 2 episodes of social behaviors whereas wild-type mice display 44 episodes including social grooming, mounting, tail pulling and sniffing; however, the frequency of self-grooming is not significantly different between wild-type and mutant mice (41 vs 36 episodes, respectively)
- when paired against each other in the social dominance tube test, homozygotes display a significantly lower percentage of wins than sex-matched wild-type mice (~30% vs 73%, respectively)

abnormal whisker trimming behavior
- homozygotes do not trim/barber their cagemates' whiskers or facial hair, unlike the majority of separately housed wild-type littermates which lack whiskers and have trimmed facial hair
- ll sets of whiskers and facial hair within 2-4 weeks after mixed genotype housing and have their whiskers trimmed within 2 weeks of return to their home cage
- when housed separately as uniform genotypes, all post-weaning homozygotes between 2 and 9 months of age display full sets of whiskers and facial hair, relative to only 25%-50% of age-matched wild-type controls
- when housed with wild-type mice, homozygotes lose all whiskers and facial hair in 5 of 11 cages after mixed housing and regrow their whiskers within 2 weeks after returning to their home cage; in contrast, whiskerless wild-type mice consistently regrow full sets of whiskers and facial hair within 2-4 weeks after mixed genotype housing and have their whiskers trimmed within 2 weeks of return to their home cage

decreased startle reflex
- homozygotes display attenuated responses to acoustic startle stimuli relative to wild-type mice
- Normal - in contrast, tactile startle reponses remain unaffected

mammalian phenotype

behavior/neurological phenotype
- Normal - homozygotes are viable, fertile and display no motor deficits or differences in pain sensitivity relative to wild-type mice
- Normal - no significant differences in spatial learning and memory are observed

hearing/vestibular/ear phenotype
- Normal - homozygotes exhibit normal stereocilia orientation at E18.5

nervous system phenotype
- Normal - homozygotes display normal brain histology and synaptic plasticity relative to wild-type mice

nervous system phenotype

decreased prepulse inhibition
- homozygotes display significantly lower levels of acoustic pre-pulse inhibition of both acoustic and tactile startle responses relative to wild-type mice

reduced sensorimotor gating
- homozygotes display significantly lower levels of acoustic pre-pulse inhibition of both acoustic and tactile startle responses relative to wild-type mice

References

Lijam N; Paylor R; McDonald MP; Crawley JN; Deng CX; Herrup K ; Stevens KE ; Maccaferri G ; McBain CJ ; Sussman DJ ; Wynshaw-Boris A. 1997. Social interaction and sensorimotor gating abnormalities in mice lacking Dvl1. Cell 90(5):895-905PubMed: 9298901 MGI: J:42758

Additional - Dvl1^tm1Awb related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Dvl1
- Genotyping resources and troubleshooting
Breeding Considerations

When maintaining a live colony, these mice can be bred as homozygotes.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the B6.129S6-Dvl1^tm1Awb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007969 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Pricing & Availability

Cryo Recovery

Domestic
International

Live Mouse

Age

Genotype

Price

weeks

Cryorecovery - Pricing

Service/Product	Description	Price
	Heterozygous for Dvl1<tm1Awb>

Related Products and Services

Frozen Mouse Embryo	B6.129S6-Dvl1<tm1Awb>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.129S6-Dvl1<tm1Awb>/J Frozen Embryo	$2595.00
Frozen Mouse Embryo	B6.129S6-Dvl1<tm1Awb>/J Frozen Embryo	$3373.50
Frozen Mouse Embryo	B6.129S6-Dvl1<tm1Awb>/J Frozen Embryo	$3373.50

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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Control Suggestions

Additional Information

Selected References

Dvl1tm1Awb

Allele Symbol: Dvl1tm1Awb

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Dvl1tm1Awb/Dvl1tm1Awbinvolves: 129S6/SvEvTac

behavior/neurological phenotype

mammalian phenotype

nervous system phenotype

References

Additional - Dvl1tm1Awb related

Genotyping Protocols

Breeding Considerations

Mating System

Citation

Animal Health Reports

Production of mice from cryopreserved embryos or sperm occurs in a maximum barrier room, G200

Live Mouse

Cryorecovery - Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Licensing Information

JAX® Mice, Products & Services Conditions of Use

No Warranty

Credit for PRODUCTS or SERVICES

Animal Care and Use for SERVICES

No Liability

Dvl1^tm1Awb

Allele Symbol: Dvl1^tm1Awb

Genotype: Dvl1^tm1Awb/Dvl1^tm1Awb
involves: 129S6/SvEvTac

Additional - Dvl1^tm1Awb related