Mice that are homozygous for this targeted mutation on the 129S genetic background exhibit diminished social interaction behavior, do not barber or trim whiskers of cagemates, show subordinance in social dominance tests, do not sleep huddled together and do not build nests. This mutant mouse strain may be useful in studies of social behavior, sensorimotor gating, and possibly psychiatric disorders.
Dr. Anthony Wynshaw-Boris, Case Western Reserve University, School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Null/Knockout) | Dvl1 | dishevelled segment polarity protein 1 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by Western blot analysis of MEF (mouse embryonic fibroblasts) derived from homozygotes. Homozygotes exhibit diminished social interaction behavior, do not barber or trim whiskers of cagemates, show subordinance in social dominance tests, do not sleep huddled together and do not build nests. Although mutants have an attenuated prepulse inhibition of acoustic and tactile startle (sensorimotor gating of the startle reflex), they do not exhibit any deficits in locomotor or cognitive function. This mutant mouse strain may be useful in studies of social behavior, sensorimotor gating, and possibly psychiatric disorders.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt part of exon 2 and exons 3 and 4, encoding amino acids 131-225. The construct was electroporated into 129S6/SvEvTac derived TC-1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting chimeric animals were crossed to mice, and then backcrossed to C57BL/6J for 15 generations.
Allele Name | targeted mutation 1, Anthony Wynshaw-Boris |
---|---|
Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Dvl1-; Dvl1del131-225 |
Gene Symbol and Name | Dvl1, dishevelled segment polarity protein 1 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 4 |
Molecular Note | A neomycin selection cassette replaced a genomic fragment containing part of exon 2 and exons 3 and 4, which encode amino acids 131-225. Western blot analysis on embryonic fibroblasts derived from homozygous mice confirmed that no detectable protein was produced from this allele. |
Mutations Made By | Dr. Anthony Wynshaw-Boris, Case Western Reserve University, School of Medicine |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129S6-Dvl1tm1Awb/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007969 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Dvl1<tm1Awb> |
Frozen Mouse Embryo | B6.129S6-Dvl1<tm1Awb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Dvl1<tm1Awb>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129S6-Dvl1<tm1Awb>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129S6-Dvl1<tm1Awb>/J Frozen Embryo | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.