Mice that are homozygous for this mutation may be useful in studies of thrombosis and platelet aggregation. Thrombin-treated mutants exhibit reduced pathological thrombosis and impaired platelet aggregation.
Greg Lemke, The Salk Institute
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Tyro3 | TYRO3 protein tyrosine kinase 3 |
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (protein) is detected by Western blot analysis of brain lysate. Homozygotes exhibit decreased sensitivity to induced pathological thrombosis
resulting in reduced fatal pulmonary embolism (25% of wild-type). Platelet aggregation and clot retraction after thrombin treatment is impaired. Platelet signaling and secretion is also defective. Activity induced seizures can occur in mutant mice over the age of seven months. This mutant mouse strain may be useful in studies of thrombosis and platelet aggregation.
A targeting vector containing neomycin resistance and herpes simplex virus thymidine kinase genes was used to disrupt exon 9 and part of exon 10. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The resulting animals were crossed to C57BL/6 mice and then made homozygous. The mice were then backcrossed to C57BL/6 for five generations.
Allele Name | targeted mutation 1, Greg Lemke |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | Rse-; Tyro3 |
Gene Symbol and Name | Tyro3, TYRO3 protein tyrosine kinase 3 |
Gene Synonym(s) | |
Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl+ |
Chromosome | 2 |
Molecular Note | A neomycin selection cassette replaced sequences containing exon 9 and part of exon 10. Western blot analysis on brain lysates derived from homozygous mice confirmed that no detectable protein was produced from this allele. |
Mutations Made By | Greg Lemke, The Salk Institute |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129-Tyro3tm1Grl/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007937 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wildtype for Tyro3<tm1Grl> |
Frozen Mouse Embryo | B6.129-Tyro3<tm1Grl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Tyro3<tm1Grl>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6.129-Tyro3<tm1Grl>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6.129-Tyro3<tm1Grl>/J Frozen Embryo | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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