These En2flox-taulacZ (En2-floxLacZ, En2tau-lacZ, or En2-tau-lacZ) mutant mice harbor a loxP-flanked tau β-galactosidase "knock-in" allele that also abolishes engrailed 2 (En2) gene function, and may be useful for conditional reporter protein expression in En2-expressing tissues (including the early mesencephalon and rhombomere 1, and developing cerebellum and jaw muscles), as well as for conditional restoration of En2 function.
Dr. Alexandra L Joyner, Memorial Sloan-Kettering Cancer Center
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted (Conditional ready (e.g. floxed), No functional change) | En2 | engrailed 2 |
Mice homozygous for this En2flox-taulacZ (also called En2-floxLacZ, En2tau-lacZ, or En2-tau-lacZ) mutation are viable but may not breed well (reported as "semi-fertile"). These mice harbor a loxP-flanked tau β-galactosidase "knock-in" allele that also abolishes engrailed 2 (En2) gene function. While both the tau-lacZ and En2 transcripts are made, only tau-lacZ is translated to protein. Expression of lacZ mimics that of the endogenous En2 gene. When bred to mice that express Cre recombinase, the resulting offspring will have the lacZ reporter deleted in the cre-expressing tissue(s) with subsequent restoration of En2 translation. These En2flox-taulacZ mutant mice may be useful for conditional reporter protein expression in En2-expressing tissues (including the early mesencephalon and rhombomere 1, and developing cerebellum and jaw muscles), as well as for conditional restoration of En2 function.
Of note, these mice may also be useful in conjunction with other engrailed mutants (such as Stock No. 007912, Stock No. 007916, Stock No. 007917, Stock No. 007918, and Stock No. 007924).
A targeting vector was designed to insert a loxP-flanked tau-lacZ cassette followed by a neo cassette and then a third loxP site into the 5'-UTR of the targeted gene. This construct was electroporated into 129S6/SvEvTac-derived W4 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts and chimeric mice were bred with Black Swiss Webster mice. The resulting offspring were then bred to cre expressing mice (on a Swiss Webster genetic background) to remove the neo cassette, generating En2flox-taulacZ offspring (also called En2-floxLacZ in Sgaier 2005 or En2tau-lacZ and En2-tau-lacZ in Sgaier 2007). These En2flox-taulacZ mutant mice were bred to Swiss Webster mice for many generations prior to arrival at The Jackson Laboratory. In addition, the donating investigator reports that although these mice harbor no additional mutant loci, they may contain incidental genetic background contributions as a result of past matings with other mutant mouse strains. Upon arrival, En2flox-taulacZ mice were bred to 129S1/SvImJ (Stock No. 002448) for at least one generation to establish the colony.
Expressed Gene | lacZ, beta-galactosidase, E. coli |
---|---|
Site of Expression | LoxP-flanked tau ?-galactosidase expression mimics and blocks that of the endogenous targeted gene. When bred to Cre recombinase mice, resulting offspring will have the lacZ reporter deleted in the cre-expressing tissue(s) with subsequent restoration of the targeted gene's translation. |
Allele Name | targeted mutation 5.1, Alexandra L Joyner |
---|---|
Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | En2flox-taulacZ; En2loxlz; En2tau-lacZ; En2-floxLacZ; En2-tau-lacZ |
Gene Symbol and Name | En2, engrailed 2 |
Gene Synonym(s) | |
Expressed Gene | lacZ, beta-galactosidase, E. coli |
Site of Expression | LoxP-flanked tau ?-galactosidase expression mimics and blocks that of the endogenous targeted gene. When bred to Cre recombinase mice, resulting offspring will have the lacZ reporter deleted in the cre-expressing tissue(s) with subsequent restoration of the targeted gene's translation. |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 5 |
Molecular Note | A targeting vector was designed to insert a loxP-flanked tau-lacZ cassette followed by a neo cassette and then a third loxP site into the 5'-UTR of the targeted gene. Cre mediated recombination then removed the neo cassette leaving the floxed tau-lacZ cassette intact. |
Mutations Made By | Dr. Alexandra Joyner, Memorial Sloan-Kettering Cancer Center |
When maintaining a live colony, heterozygous mice may be bred together or with wildtype siblings. The donating investigator reports that homozygous mice are "semi-fertile" and do not breed well.
When using the STOCK En2tm5.1Alj/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007925 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous or wild-type for En2<tm5.1Alj> |
Frozen Mouse Embryo | STOCK En2<tm5.1Alj>/J | $2595.00 |
Frozen Mouse Embryo | STOCK En2<tm5.1Alj>/J | $2595.00 |
Frozen Mouse Embryo | STOCK En2<tm5.1Alj>/J | $3373.50 |
Frozen Mouse Embryo | STOCK En2<tm5.1Alj>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.