These Fasfl mice may be useful in generating conditional mutations for studying many aspects of immune function. For example, deletion of Fas in T cells can lead to pulmonary fibrosis (model of human idiopathic pulmonary fibrosis), whereas deletion of Fas in B cells can result in a lymphoproliferative disorder.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Conditional ready (e.g. floxed), No functional change) | Fas | Fas (TNF receptor superfamily member 6) |
Mice homozygous for this "Fasfl" conditional allele are viable and fertile, with loxP sites flanking exon 9 of the targeted gene. When bred to mice that express Cre recombinase, exon 9 (which encodes the death domain) is deleted in the cre-expressing tissues in the resulting offspring.
These Fasfl mice may be useful in generating conditional mutations for studying many aspects of immune function. For example, when Fasfl mice are crossed to a strain expressing Cre recombinase in B lineage cells (see Stock No. 004126 or 006785 ), this mutant mouse strain may be useful in studies of lymphoproliferative disorder. Similarly, when Fasfl mice are crossed to an interferon inducible strain with widespread Cre recombinase expression (see Stock No. 003556, 005673, or 002527), this mutant mouse strain may be useful in studies of lymphoproliferative disease. Further, deletion of Fas in T cells (by crossing Fasfl mice to a CD4-cre transgenic strain) produces mice that develop progressive lymphopenia and inflammatory pulmonary fibrosis which resembles idiopathic pulmonary fibrosis in humans.
A targeting vector was designed to place a loxP-flanked neo cassette downstream, and a third loxP site upstream of exon 9 of the targeted gene. The construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre-expressing plasmid to remove the neo selection cassette. Chimeric mice were bred to C57BL/6 mice. The resulting "Fasfl" mice (with a single loxP site just upstream, and a single loxP site just downstream of exon 9) were maintained as homozygotes on the C57BL/6 genetic background prior to arrival at The Jackson Laboratory.
Allele Name | targeted mutation 1, University of Cologne |
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Allele Type | Targeted (Conditional ready (e.g. floxed), No functional change) |
Allele Synonym(s) | fasfl |
Gene Symbol and Name | Fas, Fas (TNF receptor superfamily member 6) |
Gene Synonym(s) | |
Strain of Origin | B6.Cg-Thy1a |
Chromosome | 19 |
Molecular Note | Exon 9, the death domain encoding exon, was flanked by loxP sites via homologous recombination. |
Mutations Made By | Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine |
When maintaining a live colony, homozygous mice may be bred together.
When using the C57BL/6-Fastm1Cgn/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007895 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Fas<tm1Cgn> |
Frozen Mouse Embryo | C57BL/6-Fas<tm1Cgn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Fas<tm1Cgn>/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | C57BL/6-Fas<tm1Cgn>/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | C57BL/6-Fas<tm1Cgn>/J Frozen Embryo | $3373.50 |
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