C57BL/6-Fas^tm1Cgn/J

Stock number: 007895
Research areas: Cell Biology Research, Research Tools

Description

These Fas^fl mice may be useful in generating conditional mutations for studying many aspects of immune function. For example, deletion of Fas in T cells can lead to pulmonary fibrosis (model of human idiopathic pulmonary fibrosis), whereas deletion of Fas in B cells can result in a lymphoproliferative disorder.

Detailed description

Mice homozygous for this "Fas^fl" conditional allele are viable and fertile, with loxP sites flanking exon 9 of the targeted gene. When bred to mice that express Cre recombinase, exon 9 (which encodes the death domain) is deleted in the cre-expressing tissues in the resulting offspring.

These Fas^fl mice may be useful in generating conditional mutations for studying many aspects of immune function. For example, when Fas^fl mice are crossed to a strain expressing Cre recombinase in B lineage cells (see Stock No. 004126 or 006785 ), this mutant mouse strain may be useful in studies of lymphoproliferative disorder. Similarly, when Fas^fl mice are crossed to an interferon inducible strain with widespread Cre recombinase expression (see Stock No. 003556, 005673, or 002527), this mutant mouse strain may be useful in studies of lymphoproliferative disease. Further, deletion of Fas in T cells (by crossing Fas^fl mice to a CD4-cre transgenic strain) produces mice that develop progressive lymphopenia and inflammatory pulmonary fibrosis which resembles idiopathic pulmonary fibrosis in humans.

Development

A targeting vector was designed to place a loxP-flanked neo cassette downstream, and a third loxP site upstream of exon 9 of the targeted gene. The construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Correctly targeted ES cells were transiently transfected with a cre-expressing plasmid to remove the neo selection cassette. Chimeric mice were bred to C57BL/6 mice. The resulting "Fas^fl" mice (with a single loxP site just upstream, and a single loxP site just downstream of exon 9) were maintained as homozygotes on the C57BL/6 genetic background prior to arrival at The Jackson Laboratory.

Allele

Symbol: Fas^tm1Cgn
Name: targeted mutation 1, University of Cologne
MGI accession ID: MGI:3690464
Generation method: Targeted
Attributes: Conditional ready (e.g. floxed); No functional change
Marker symbol: Fas
Marker name: Fas (TNF receptor superfamily member 6)
Chromosome: 19
Strain of origin: B6.Cg-Thy1^a
Molecular note: Exon 9, the death domain encoding exon, was flanked by loxP sites via homologous recombination.