These Mir150 knock-out mice may be useful in mircoRNA biology, specifically to study the role of miR-150 and its target genes (including Myb) in lymphocyte development and function.
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
Mice homozygous for this knock-out allele (Mir150tm1Rsky) are viable and fertile with normal development of T cells, follicular B cells, and MZ B cells. No miR-150 is expressed in spleen, mesenteric lymph node, and thymus of homozygotes. Homozygous mice exhibit B cell expansion (CD19+B220loCD5+CD43+CD23-; B1a subset) in spleen and peritoneal cavity (with reciprocal reduction in B2 cells) and enhanced humoral immune response (increased serum immunoglobulins of various classes both at steady-state and following T cell-dependent antigen exposure). Homozygous miR-150 deficiency also leads to enhanced induction of the miR-150 target protein c-Myb in activated B and T cells, but no reported change in expression of the miR-150 target genes Foxp1 or ZFP91 in resting or activated B cells. These Mir150tm1Rsky mice may be useful in mircoRNA biology, specifically to study the role of miR-150 and its target genes (including Myb) in lymphocyte development and function.
A targeting vector was designed to insert an frt-flanked pGK-Neo cassette 468 bp upstream of the miR-150 stem loop, with one loxP site placed immediately upstream of the pGK-Neo cassette and the other loxP site placed 146 bp downstream of the miR-150 stem loop. The construct was electroporated into C57BL/6-derived Bruce-4 embryonic stem (ES) cells. Chimeric mice were bred to C57BL/6 mice. To delete the loxP site-flanked 679 bp miR-150 coding genomic region and the frt-flanked pGK-Neo cassette, mice were then bred to cre-expressing mice (C57BL/6 congenic background (originally BALB/c), similar to Stock No. 006054). The resulting mice, with a single loxP site replacing the miR-150 coding region, were bred together (selecting against the cre transgene) to obtain miR150-/- homozygotes.
|Allele Name||targeted mutation 1, Klaus Rajewsky|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Mir150, microRNA 150|
|Strain of Origin||B6.Cg-Thy1a|
|Molecular Note||A targeting vector was designed to insert an frt-flanked pGK-Neo cassette 468 bp upstream of the miR-150 stem loop, with one loxP site placed immediately upstream of the pGK-Neo cassette and the other loxP site placed 146 bp downstream of the miR-150 stem loop. To delete the loxP site-flanked 679 bp miR-150 coding genomic region and the frt-flanked pGK-Neo cassette, mice were then bred to cre-expressing mice (C57BL/6 congenic background, similar to Stock No. 006054). The resulting mice have a single loxP site replacing the miR-150 coding region.|
|Mutations Made By|| |
Klaus Rajewsky, Max Delbruck Centre for Molecular Medicine
When maintaining a live colony, homozygous mice may be bred together.
When using the B6(C)-Mir150tm1Rsky/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007750 in your Materials and Methods section.
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