B6.NZB-(D1Mit47-D1Mit209)/BkotJ

Stock number: 007716
Strain synonyms: B6.Nba2
Research areas: Immunology, Inflammation and Autoimmunity Research

Description

Homozygotes for this NZB-derived lupus susceptibility QTL (Nba2) develop antinuclear antibodies and proteinuria, but do not develop fatal nephritis. This strain may be useful in dissecting the genetics of systemic lupus erythematosus.

Detailed description

Mice homozygous for the NZB/BlNJ-derived lupus susceptibility QTL (Nba2) on the C57BL/6 background develop elevated serum IgG antinuclear antibodies, a two-fold increase in the number of spleen cells (by 10 weeks of age), an increase in the absolute number of B and T cells, early B cell activation, increased levels of IgM, a two-fold increase in activated (CD69⁺) B cells and a two fold increase in FAS+ B cells. Although approximately 10% of females older than 10 months of age develop high levels of proteinuria, the subsequent nephritis does not result in death. When crossed with NZW/LacJ mice, 95% of F1 hybrid females develop elevated autoantibodies, severe proteinuria and fatal nephritis. This strain may be useful in dissecting the genetics of systemic lupus erythematosus.

Development

This congenic interval, defined by D1Mit47-D1Mit209, was introgressed into C57BL/6J from the donor strain NZB/BlNJ for a minimum of 12 generations and then intercrossed to generate homozygotes. It is maintained by sibling matings. The QTL Nba2 (New Zealand Black autoimmunity 2) is within this interval, and is associated with hypergammaglobulinemia and autoantibody production. The strain was donated to The Jackson Laboratory by Dr. Brian Kotzin in 2007.

Allele

Symbol: Nba10^NZB
Name: NZB
MGI accession ID: MGI:3529870
Generation method: QTL
Marker symbol: Nba10
Marker name: New Zealand Black autoimmunity 10
Marker synonyms: Nba2
Chromosome: 1
Strain of origin: NZB
Molecular note: This allele confers increased susceptibility to autoimmunity compared to C57BL/6.
General note: This allele shows linkage to increased anti-chromatin and anti-DNA antibodies, increased serum gp70 immune complex levels, and severe glomerulonephritis.

Allele

MGI accession ID: MGI:91696
Marker symbol: D1Mit47
Marker name: DNA segment, Chr 1, Massachusetts Institute of Technology 47
Chromosome: 1

Allele

MGI accession ID: MGI:91588
Marker symbol: D1Mit209
Marker name: DNA segment, Chr 1, Massachusetts Institute of Technology 209
Chromosome: 1