Overview

Also Known As:mT/mG , mTmG

mT/mG mice (Stock No. 007676) have the same allele as Stock No. 007576 but has a C57BL/6J congenic background. ROSA^mT/mG is a cell membrane-targeted, two-color fluorescent Cre-reporter allele. Prior to Cre recombination, cell membrane-localized tdTomato (mT) fluorescence expression is widespread in cells/tissues. Cre recombinase expressing cells (and future cell lineages derived from these cells) have cell membrane-localized EGFP (mG) fluorescence expression replacing the red fluorescence. ROSA^mT/mG mice (Stock No. 007576 and Stock No. 007676) function similarly to ROSA^nT-nG mice (Stock No. 023035 and Stock No. 023537), with ROSA^mT/mG directed to cell membrane and ROSA^nT-nG directed to nuclei.

Donating Investigator

Liqun Luo, Stanford University

Genetic overview

Genetic Background	Generation
	N12F1 (2021-07-22 00:00:00)

Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}

Allele Type	Gene Symbol	Gene Name
Targeted (Conditional ready (e.g. floxed), Reporter)	Gt(ROSA)26Sor	gene trap ROSA 26, Philippe Soriano

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Research Applications

Neurobiology Research
Research Tools

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Base Price

Starting at:

$236.78 Domestic price for female 4-week

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Details

Detailed Description

Mice homozygous for this mT/mG mutation are viable and fertile. These mice possess loxP sites on either side of a membrane-targeted tdTomato (mT) cassette and express strong red fluorescence in all tissues and cell types examined. Tail or whole body epifluorescence is sufficient to identify mT/mG mice from wildtype mice. When bred to Cre recombinase expressing mice, the resulting offspring have the mT cassette deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG) cassette located just downstream. The donating investigator reports that the ACTB promoter allows stronger and persistent expression of the fluorescent proteins (especially in adult cells) compared to the endogenous Gt(ROSA) locus alone. This double-fluorescent system allows direct live visualization of both recombined and non-recombined cells at single cell resolution, offering an internal control for phenotypic analysis of Cre-induced mosaic mutants and providing a second marker for lineage tracing applications. In addition, the localization of fluorescent proteins to membrane structures outlines cell morphology and allows resolution of fine cellular processes. These mT/mG mice are useful as a Cre reporter strain; expressing red fluorescence prior to, and green fluorescence following, Cre-mediated recombination in widespread cell and tissue types.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

The mT/mG (membrane-Tomato/membrane-Green) targeting vector was designed with a CMV enhancer/chicken beta-actin core promoter (pCA) driving expression of a loxP-flanked, N-terminal membrane-tagged, tdTomato protein sequence followed by a polyadenylation signal (tdTomato is a non-oligomerizing DsRed variant with a 12 residue linker fusing two copies of the protein (tandem dimer)). Immediately distal to the second loxP site is an N-terminal membrane-tagged, enhanced green fluorescent protein (EGFP) sequence itself followed by a polyadenylation signal. An frt-flanked neo cassette was also located distal to the expression vector. This entire mT/mG construct was inserted into the Gt(ROSA)26Sor locus via electroporation of (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected in C57BL/6J blastocysts. Chimeric progeny were bred to outbred CD-1 mice. The resulting mutant mice were interbred to generate homozygotes prior to arrival at The Jackson Laboratory (as Stock No. 007576). Upon arrival, some mice were backcrossed to C57BL/6J for at least five generations to generate this congenic strain (Stock No. 007676).

Expression Data

Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	Membrane-targeted tdTomato (mT) is expressed in all tissues and cell types examined. When bred to Cre recombinase expressing mice, the mT cassette is deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG).

Control Suggestions

000664 C57BL/6J

Additional Information

Considerations for Choosing Controls

Selected References

Muzumdar MD; Tasic B; Miyamichi K; Li L; Luo L. 2007. A global double-fluorescent Cre reporter mouse. Genesis 45(9):593-605PubMed: 17868096 MGI: J:124702

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Genetics

Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}

Allele Symbol: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}

Allele Name	targeted mutation 4, Liqun Luo
Allele Type	Targeted (Conditional ready (e.g. floxed), Reporter)
Allele Synonym(s)	Gt(ROSA26)^{ACTB-tdTomato-EGFP}; mT/mG; mTmG; mTomato/mEGFP; R26^TG; R26-mR/mG; R26-X; ROSA^mT-mE; Rosa^mTmG; Rosa26^{flox-mTRed-Stop-flox-mGFP}; Rosa26^mTmG; ROSA26-mTmG; Rosa26mTmG^flox; Rosa26R^mTmG; ROSA-mT/mG; ROSA-R/G; TomatoGFP+
Gene Symbol and Name	Gt(ROSA)26Sor, gene trap ROSA 26, Philippe Soriano
Gene Synonym(s)
Expressed Gene	GFP, Green Fluorescent Protein,
Expressed Gene	RFP, Red Fluorescent Protein, coral
Site of Expression	Membrane-targeted tdTomato (mT) is expressed in all tissues and cell types examined. When bred to Cre recombinase expressing mice, the mT cassette is deleted in the cre expressing tissue(s), allowing expression of the membrane-targeted EGFP (mG).
Strain of Origin	(129X1/SvJ x 129S1/Sv)F1-Kitl⁺
Chromosome	6
Molecular Note	The targeting vector was designed with a CMV enhancer/chicken beta-actin core promoter (pCA) driving expression of a loxP-flanked, N-terminal membrane-tagged, optimized DsRed fluorescent protein variant (called tandem-dimer-Tomato or tdTomato) sequence followed by a polyadenylation signal. Immediately distal to the second loxP site is an N-terminal membrane-tagged, enhanced green fluorescent protein (EGFP) sequence itself followed by a polyadenylation signal. An frt-flanked neo cassette was also located distal to the expression vector. Red fluorescence is detected in all tissues tested. When mice are bred to Cre expressing mice, the floxed region is excised in Cre-expressing tissue and this allows expression of the EGFP cassette.
Mutations Made By	Liqun Luo, Stanford University

Disease/Phenotype

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Neurobiology Research
- Cre-lox System
  - loxP-flanked Sequences: Test/Reporter
  - loxP-flanked Sequences
Research Tools
- Genetics Research
  - Mutagenesis and Transgenesis
  - Mutagenesis and Transgenesis: Cre-lox System
  - Tissue/Cell Markers: Cre-lox System
  - Tissue/Cell Markers
  - Tissue/Cell Markers: multiple
- Cre-lox System
  - loxP-flanked Sequences
  - loxP-flanked Sequences: Test/Reporter
- Fluorescent Proteins

Mammalian Phenotype Terms by Genotype

The following phenotype information is associated with a similar, but not exact match to this JAX^® Mice strain

Genotype: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}
involves: 129S1/Sv * 129X1/SvJ

normal phenotype

no abnormal phenotype detected
- Normal - mice are viable and fertile, with no observable adverse phenotypes

References

Muzumdar MD; Tasic B; Miyamichi K; Li L; Luo L. 2007. A global double-fluorescent Cre reporter mouse. Genesis 45(9):593-605PubMed: 17868096 MGI: J:124702

Additional - Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo} related

Technical Support

CONTACT TECHNICAL SUPPORT

Genotyping Protocols
- Standard PCR:Gt(Rosa)26sor(Neo)-alternate1
- Genotyping resources and troubleshooting
Dietary Information
- LabDiet® 5K52 formulation (6% fat)
Breeding Considerations

Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, homozygous mice may be bred together.
- Additional Breeding and Husbandry Support
Mating System
- Homozygote x Homozygote
Citation
When using the mT/mG , mTmG mouse strain in a publication, please cite the originating article(s) and include JAX stock #007676 in your Materials and Methods section.

Animal Health Reports

Facility Barrier Level Descriptions

AX10 (Standard)

Pricing & Availability

Available Now

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Live Mouse

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Genotype

Price

weeks

Cryorecovery - Not-For-Profit & Academic Pricing

Service/Product	Description	Price
> >	Heterozygous for Gt(ROSA)26Sor<tm4(ACTB-tdTomato,-EGFP)Luo>

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Frozen Mouse Embryo	B6.129(Cg)-Gt(ROSA)26Sor<tm4(ACTB-tdTomato -EGFP)Luo>/J Froz	$2595.00
Frozen Mouse Embryo	B6.129(Cg)-Gt(ROSA)26Sor<tm4(ACTB-tdTomato -EGFP)Luo>/J Froz	$2595.00
Frozen Mouse Embryo	B6.129(Cg)-Gt(ROSA)26Sor<tm4(ACTB-tdTomato -EGFP)Luo>/J Froz	$3373.50
Frozen Mouse Embryo	B6.129(Cg)-Gt(ROSA)26Sor<tm4(ACTB-tdTomato -EGFP)Luo>/J Froz	$3373.50

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Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.

The Jackson Laboratory's Genotype Promise

The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.

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General Terms and Conditions

Questions about Terms of Use

Additional Use Restrictions Apply

Not Available to Companies or for Commercial Use

Use of MICE only available to non-profit entities.

Licensing Information

Phone: 207-288-6470

Email: TechTran@jax.org

Also Known As:mT/mG , mTmG

Donating Investigator

Genetic overview

Research Applications

Base Price

Detailed Description

Development

Expression Data

Control Suggestions

Additional Information

Selected References

Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo

Allele Symbol: Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo

Disease Terms

Research Areas By Phenotype

This mouse can be used to support research in many areas including:

Mammalian Phenotype Terms by Genotype

Genotype: Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luoinvolves: 129S1/Sv * 129X1/SvJ

normal phenotype

References

Additional - Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo related

Genotyping Protocols

Dietary Information

Breeding Considerations

Mating System

Citation

Animal Health Reports

Live Mouse

Cryorecovery - Not-For-Profit & Academic Pricing

Related Products and Services

Payment Terms and Conditions

The Jackson Laboratory's Genotype Promise

Terms Of Use

Additional Use Restrictions Apply

Licensing Information

Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}

Allele Symbol: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}

Genotype: Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}/Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo}
involves: 129S1/Sv * 129X1/SvJ

Additional - Gt(ROSA)26Sor^{tm4(ACTB-tdTomato,-EGFP)Luo} related