Homozygotes for the Smad1tm2Sor (also called Smad1L) allele have abnormal gastic mucosal cell homeostasis and fewer primordial germ cells than wildtype controls. This mutant mouse strain may be useful in studies of stomach development, gastic mucosal homeostasis and BMP and MAPK signaling pathways during development and in the adult.
Dr. Philippe Soriano, Mount Sinai School of Medicine
Genetic Background | Generation |
---|---|
|
Allele Type | Gene Symbol | Gene Name |
---|---|---|
Targeted | Smad1 | SMAD family member 1 |
Homozygotes for the Smad1tm2Sor (also called Smad1L) allele are viable and fertile, normal in size and do not display any gross physical or behavioral abnormalities. These mice carry a mutation in exon 3, which effects MAPK-mediated phosphorylation of the protein. Western blot analysis of MEFs from homozygotes showed that similar protein levels compared to wildtype. Homozygous embryos have fewer primordial germ cells than wildtype controls. Homozygous mice display abnormal gastric mucosa cell population ratios with fewer zymogenic cells and more parietal cells. The cytoskeleton of MEFs from homozygotes exhibit a loss of adhesion zippers, decreased stress fibers, and an accumulation of actin in the cortical regions with an increase in beta-catenin immunostaining localized to the cell membranes. This mutant mouse strain may be useful in studies of stomach development, gastic mucosal homeostasis and BMP and MAPK signaling pathways during development and in the adult.
A targeting vector containing a floxed PGKneo cassette and a diptheria toxin cassette was used to introduce serine-to-alanine substitutions in the MAPK-consensus phosphorylation sites (S187A, S195A, S206A, S214A) and two conserved phosphoserines in exon 3 (S209 and S210). The construct was electroporated into 129S4/SvJaeSor-derived AK7 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient blastocysts. The mice were crossed to a strain expressing Cre under the control of the Meox2 promoter to remove the floxed PGKneo cassette. The mice were then crossed to mice carrying the Smad1tm1Sor mutant allele prior to arriving at The Jackson Laboratory. Upon arrival, mice were bred to C57BL/6J to separate the mutant alleles. These mice carry only the Smad1tm2Sor (also called Smad1L) mutant allele.
Allele Name | targeted mutation 2, Philippe Soriano |
---|---|
Allele Type | Targeted |
Allele Synonym(s) | Smad1L |
Gene Symbol and Name | Smad1, SMAD family member 1 |
Gene Synonym(s) | |
Promoter | Smad1, SMAD family member 1, mouse, laboratory |
Strain of Origin | 129S4/SvJaeSor |
Chromosome | 8 |
Molecular Note | Homologous recombination incorporated serine to alanine substitutions in the MAPK-consensus phosphorylation sites (p.S187A, p.S195A, p.S206A, p.S214A) and two conserved phosphoserines in exon 3 (p.(S209_S210delinsAA)). Western blot analysis of MEF nuclear extracts demonstrated the mutant protein was expressed at similar levels as that of wild-type. |
Mutations Made By | Dr. Philippe Soriano, Mount Sinai School of Medicine |
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6;129-Smad1tm2Sor/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007613 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Smad1<tm2Sor> |
Frozen Mouse Embryo | B6;129-Smad1<tm2Sor>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Smad1<tm2Sor>/J | $2595.00 |
Frozen Mouse Embryo | B6;129-Smad1<tm2Sor>/J | $3373.50 |
Frozen Mouse Embryo | B6;129-Smad1<tm2Sor>/J | $3373.50 |
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
What information were you hoping to find through your search?
How easy was it to find what you were looking for?
We may wish to follow up with you. Enter your email if you are happy for us to connect and reachout to you with more questions.
Please Enter a Valid Email Address
Thank you for sharing your feedback! We are working on improving the JAX Mice search. Come back soon for exciting changes.