These Pax8-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in renal tubular epithelial cells, and may be useful in studying renal disorders such as fibrosis or polycystic kidney disease, renal cancer, and Tuberous Sclerosis (along with Stock No. 005680).
IMR Colony, The Jackson Laboratory
Genetic Background | Generation |
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Allele Type |
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Transgenic (Transactivator) |
Transgenic Pax8-rtTA mice are viable and fertile. These mice express an optimized reverse tetracycline-controlled transactivator (rtTA2S-M2) protein under the control of the murine Pax8 promoter, which directs expression to proximal and distal tubules and the collecting duct system of both embryonic and adult kidney. The rtTA2S-M2 variant of rtTA contains five amino acid changes in the TetR moiety (S12G, E19G, A56P, D148E, and H179R) and a synthetic optimized transactivating domain, resulting in reduced basal activity and enhanced doxycycline sensitivity compared to wild-type rtTA. When mated to a second strain carrying a gene of interest under the regulatory control of a tetracycline-responsive promoter element (TRE or tetO), expression of the target gene in kidney cells is induced with administration of the tetracycline analog, doxycycline (dox). These Pax8-rtTA mice provide a Tet-On tool that allows the inducible expression of genes in renal tubular epithelial cells, and may be useful in studying renal disorders such as fibrosis or polycystic kidney disease, renal cancer, and Tuberous Sclerosis.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
The Pax8-rtTA construct was designed to contain an optimized rtTA variant (rtTA2s-M2) cDNA and SV40 polyA sequence replaced at the endogenous ATG translational start site of a murine Pax8 sequence. This 6.6 kb transgene was microinjected into the pronucleus of one-cell fertilized mouse embryos obtained from superovulated F2 (C57BL/6 x DBA) females, which were then implanted into pseudopregnant foster mice. Founder mice were bred to C57BL/6 mice, and the resulting transgenic offspring were bred together for many generations prior to arrival at The Jackson Laboratory. Upon arrival, some mice were backcrossed to C57BL/6J for at least 5 generations to generate this congenic strain (Stock No. 007176). The Pax8-rtTA construct integrated approximately five copies in a head-to-tail orientation within a mouse L1 line repetitive element (which are located throughout the genome).
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
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Site of Expression | expression is directed to proximal and distal tubules and the collecting duct system of both embryonic and adult kidney. |
Allele Name | transgene insertion 1, Robert Koesters |
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Allele Type | Transgenic (Transactivator) |
Allele Synonym(s) | Pax8-rtTA |
Gene Symbol and Name | Tg(Pax8-rtTA2S*M2)1Koes, transgene insertion 1, Robert Koesters |
Gene Synonym(s) | |
Promoter | Pax8, paired box 8, mouse, laboratory |
Expressed Gene | rtTA, reverse tetracycline-controlled transactivator, E. coli |
Site of Expression | expression is directed to proximal and distal tubules and the collecting duct system of both embryonic and adult kidney. |
Strain of Origin | (C57BL/6 x DBA)F2 |
Chromosome | 8 |
Molecular Note | The Pax8-rtTA construct contains an optimized rtTA variant (rtTA2s-M2) cDNA and SV40 polyA sequence replaced at the endogenous ATG translational start site of a murine Pax8 sequence. The rtTA2S*M2 variant contains 5 amino acid changes in the TetR moiety (S12G, E19G, A56P, D148E, and H179R) and a synthetic optimized transactivating domain, resulting in reduced basal activity and enhanced doxycycline sensitivity compared to wild-type rtTA. |
Mutations Made By | Robert Koesters, Universitaetsklinikum Heidelberg |
Mutant mice were bred to C57BL/6J mice to generate this congenic strain. When maintaining the live congenic colony, hemizygous mice are bred with wildtype siblings or to C57BL/6J inbred mice.
When using the B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007176 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
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Hemizygous or Non carrier for Tg(Pax8-rtTA2S*M2)1Koes |
Frozen Mouse Embryo | B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J Frozen Embryos | $2595.00 |
Frozen Mouse Embryo | B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J Frozen Embryos | $3373.50 |
Frozen Mouse Embryo | B6.Cg-Tg(Pax8-rtTA2S*M2)1Koes/J Frozen Embryos | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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