These "Cyp 4a14" mutant mice may be useful studying kidney function and metabolism, cardiovascular physiology, hypertension, and the relationships between blood pressure, sex hormones, and p450 ω-hydroxylases.
Jorge H. Capdevila, Vanderbilt University Medical School
Genetic Background | Generation |
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|
Allele Type | Gene Symbol | Gene Name |
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Targeted (Null/Knockout) | Cyp4a14 | cytochrome P450, family 4, subfamily a, polypeptide 14 |
Mice homozygous for this "Cyp 4a14" mutant allele are viable and fertile. Homozygous deficiency of the targeted gene leads to spontaneous hypertension (more severe in males) that is androgen-sensitive. Homozygotes also exhibit other interrelated metabolic and regulatory effects; increased renal vascular resistance, impaired renal hemodynamics, elevated plasma androgens (5α-dihydrotestosterone (DHT) and testosterone), upregulated Cyp4a12 gene expression, and increased formation of prohypertensive 20-HETE. These "Cyp 4a14" mutant mice may be useful studying kidney function and metabolism, cardiovascular physiology, hypertension, and the relationships between blood pressure, sex hormones, and p450 ω-hydroxylases.
A targeting vector was designed to replace exons 10 and 11 of the targeted gene (coding for the heme-binding peptide) with a neomycin resistance cassette. The construct was electroporated into 129S6/SvEvTac-derived TL-1 embryonic stem (ES) cells. Correctly targeted ES cells were microinjected into blastocysts and the resulting chimeric mice were bred to 129S6/SvEvTac to generate heterozygotes. Mutants were then backcrossed to wildtype 129S6/SvEvTac mice for 11 generations prior to arrival at The Jackson Laboratory. Upon arrival, mice may have been bred with 129S1/SvImJ (Stock No. 002448) to establish the colony.
Allele Name | targeted mutation 1, Jorge H Capdevila |
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Allele Type | Targeted (Null/Knockout) |
Allele Synonym(s) | 4a14(-) |
Gene Symbol and Name | Cyp4a14, cytochrome P450, family 4, subfamily a, polypeptide 14 |
Gene Synonym(s) | |
Strain of Origin | 129S6/SvEvTac |
Chromosome | 4 |
Molecular Note | Exons 10 and 11, encoding the heme-binding domain, were replaced with a neomycin selection cassette. The insertion introduced a frameshift mutation at codon 404 and generated a unique HindIII restriction site. |
Mutations Made By | Jorge Capdevila, Vanderbilt University Medical School |
When maintaining a live colony, homozygous mice can be bred together.
When using the 129S-Cyp4a14tm1Jhc/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #007175 in your Materials and Methods section.
Facility Barrier Level Descriptions
Service/Product | Description | Price |
---|---|---|
Heterozygous for Cyp4a14<tm1Jhc> |
Frozen Mouse Embryo | 129S-Cyp4a14<tm1Jhc>/J | $2595.00 |
Frozen Mouse Embryo | 129S-Cyp4a14<tm1Jhc>/J | $2595.00 |
Frozen Mouse Embryo | 129S-Cyp4a14<tm1Jhc>/J | $3373.50 |
Frozen Mouse Embryo | 129S-Cyp4a14<tm1Jhc>/J | $3373.50 |
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The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project. We do not guarantee breeding performance and therefore suggest that investigators order more than one breeding pair to avoid delays in their research.
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