CByJ.B6-Prf1^tm1Sdz/J

Stock number: 007079
Research areas: Apoptosis Research, Immunology, Inflammation and Autoimmunity Research, Research Tools

Description

CTL and NK cells from mice homozygous for this targeted mutation of Prf1, perforin 1 (pore forming protein), have impaired pathogen clearance. This mutant mouse strain may be useful in studies of inflammatory response, susceptibility to infection, and susceptibility to multiple sclerosis.

Detailed description

Mice homozygous for the targeted mutation are viable and fertile. Homozygous mutant mice have normal numbers of CD8⁺ T cells and NK cells. CTL and NK cells are unable to lyse virus-infected or allogeneic fibroblasts in vitro. Homozygotes fail to clear lymphocytic choriomeningitis virus. Fibrosarcoma tumor cells are eliminated with reduced efficiency. This mutant mouse strain may be useful in studies of inflammatory response, susceptibility to infection, and susceptibility to multiple sclerosis.

In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. This is the case for the strain above. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.

Development

A replacement vector was used containing the neo gene and which resulted in the disruption of exon 3 of the perforin gene without delection of coding sequence. No fully function perforin mRNA was detected by reverse transcription PCR. Male BL/6-III ES cells were used. There cells were originally derived from C57BL/6 mice. The mice were then backcrossed five generations with BALB/cByJ inbred mice (Stock No. 001026).

Allele

Symbol: Prf1^tm1Sdz
Name: targeted mutation 1, Sandoz Pharmaceuticals
MGI accession ID: MGI:1857235
Generation method: Targeted
Attributes: Null/Knockout
Marker symbol: Prf1
Marker name: perforin 1 (pore forming protein)
Chromosome: 10
Strain of origin: C57BL/6
Molecular note: A neomycin selection cassette was inserted into exon 3. RT-PCR analysis on RNA derived from homozygous mice demonstrated that an abnormal transcript was produced from this allele. However, immunocytochemistry experiments on activated spleen cells derived from homozygous mice confirmed that no detectable protein was made from this allele.