Mice homozygous for this knock-out mutation exhibit decreased T cell proliferation and increased susceptibility to parasitic infection.
Dr. Richard A. Flavell, Yale University School of Medicine
Mice homozygous for this targeted mutation are viable and fertile and do not display any gross physical or behavioral abnormalities. Immunoblot analysis of heart ventricle RNA confirms the absence of expression. Defects in T cell activation and proliferation have been noted. This strain may also be useful in studies of cardiomyocyte physiology.
This allele involves many repeated sequences, making PCR distinction of homozygous and heterozygous genotypes impractical. Southern blot is recommended by the donating lab.
A targeting vector was designed to replace a 5.3-kb genomic fragment containing a segment of the coding region, putative promoter and 5' UTR with a loxP-flanked neomycin resistance gene expression cassette. The targeting vector was linearized and electroporated into 129S1/Sv-p+ Tyr+ Kitl+-derived W9.5 embryonic stem (ES) cells. Clones resistant to G418 and gancyclovir were selected, and homologous recombination was confirmed by Southern blotting. Clones containing the targeted mutation were injected into C57BL/6 blastocysts. Resulting male chimeric mice were bred to C57BL/6 females to obtain heterozygous mice. Heterozygotes were intercrossed to generate homozygotes. This line has been backcrossed to C57BL/6 ten times by the donating laboratory.
|Allele Name||targeted mutation 1, Vincent T Marchesi|
|Allele Type||Targeted (Null/Knockout)|
|Allele Synonym(s)||Ahnaktm1Vtm; targeted mutation 1, Vincent T Marchesi|
|Gene Symbol and Name||Ahnak, AHNAK nucleoprotein (desmoyokin)|
|Gene Synonym(s)||AHNAK 1; 2310047C17Rik; UV118; AHNAKRS; RGD1308572; 1110004P15Rik; AA589382; RIKEN cDNA 2310047C17 gene; 2310047C17Rik; AV091586; DY6; RIKEN cDNA 1110004P15 gene; expressed sequence AV091586; expressed sequence AA589382; 1110004P15Rik; PM227|
|Strain of Origin||129S1/Sv-Oca2+ Tyr+ Kitl+|
|Molecular Note||A floxed neo cassette replaced 5.3 kb of sequence encompassing the putative promoter, 5' UTR, and a segment of the coding region. Northern blot of mutant heart ventricles indicated no transcript was present, and absence of normal protein was confirmed by immunoblot.|
|Mutations Made By|| |
Vincent Marchesi, Yale University
When maintained as a live colony, homozygous crosses may be used.
When using the AHNAK KO mouse strain in a publication, please cite the originating article(s) and include JAX stock #007011 in your Materials and Methods section.
|Heterozygous or wildtype for Ahnak<tm1Vtm>|
We will fulfill your order by providing at least two carriers for each strain ordered. The total number, sex, and genotypes provided will vary, although typically 8 or more animals are provided. Please check genotypes which will be recovered. While the genotypes of all animals produced will be communicated to you prior to scheduling shipment, the genotypes of animals provided may not reflect the mating scheme and genotypes described in the strain description. Animals are typically ready to ship in 11-14 weeks. If a second recovery is required to produce the minimum number of animals, then delivery time would increase to approximately 25 weeks. If we fail to produce animals of the correct genotype, you will not be charged. We cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
|Frozen Mouse Embryo||$2,595.00 per straw or vial|
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