Mice that are homozygous for the targeted mutation exhibit slightly increased red blood cell counts, mean corpuscular hemoglobin, hematocrit and mean corpuscular volume when compared to wild-type controls. Homozygotes have diminished antigen-induced granulation tissue formation.
Dr. Stephen Hedrick, University of California at San Diego
Mice that are homozygous for the targeted mutation are viable, fertile, normal in size and do not display any gross physical or behavioral abnormalities. No gene product (mRNA) is detected by Northern blot or RT-PCR analysis. Immunohistochemical reactivity is not detected in inflamed skin. Although mutant mice exhibit slightly increased red blood cell counts, mean corpuscular hemoglobin, hematocrit and mean corpuscular volume when compared to wild-type controls, these levels are within the normal range for mice. Homozygotes have diminished antigen-induced granulation tissue formation but show normal antigen-independent granulation tissue formation. This mutant mouse strain may be useful in studies of glycosidic molecular interactions and function, antigen-specific and antigen-independent cellular immune response and hematopoiesis.
This strain was transferred from the collection of the Consortium for Functional Glycomics.
A targeting vector containing a PGKneo casssette was used to disrupt exons 2 and 3, which encode the translational start site and cytoplasmic and transmembrane domains. The construct was electroporated into (129X1/SvJ x 129S1/Sv)F1-Kitl+ derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into recipient C57BL/6J blastocysts. The resulting male chimeric animals were crossed to C57BL/6J female mice. Heterozygotes were crossed to obtain homozygotes. The mice were then backcrossed to C57BL/6 for 10 generations before arriving at The Jackson Laboratory.
|Allele Name||targeted mutation 1, Stephen M Hedrick|
|Allele Type||Targeted (Null/Knockout)|
|Gene Symbol and Name||Clec10a, C-type lectin domain family 10, member A|
|Strain of Origin||(129X1/SvJ x 129S1/Sv)F1-Kitl+|
|Molecular Note||Exons 2 and 3 were replaced with a neomycin selection cassette. No transcript was detected in homozygous mutant mice via Northern blot and RT-PCR analyses, however, immunohistochemical staining showed low levels of reactivity in peritoneal exudate cells.|
|Mutations Made By|| |
Peter Sobieszczuk, Consortium for Functional Glycomics,TSRI
When maintaining a live colony, these mice can be bred as homozygotes.
When using the B6.129-Clec10atm1Hed/J mouse strain in a publication, please cite the originating article(s) and include JAX stock #006944 in your Materials and Methods section.