Hemizygous mice for this transgene have severe plasma hypertriglyceridemia and significantly increased plasma cholesterol.
Dr. Jan L. Breslow, Rockefeller University
Genetic Background | Generation |
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?+N2pN5
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Allele Type |
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Transgenic (Inserted expressed sequence, Humanized sequence) |
Mice hemizygous for this "human apo CIII" transgene are viable and fertile. This high expressor founder line (3703) exhibits transgene expression primarily in the liver with some intestinal expression as well. Hemizygous mice have severe plasma hypertriglyceridemia and significantly increased plasma cholesterol. However, resistance to insulin-mediated glucose uptake or hyperinsulinemia are not observed. This high expressor human apo CIII transgenic strain may be useful in studying lipid metabolism, very low density lipoproteins (VLDL), hypertriglyceridemia, coronary heart disease, and/or atherosclerosis. The Donating Investigator reports that due to a possible transcribing error founder line 3703 (imported by The Jackson Laboratory in 2007) is most likely the published founder line 3707, and that plasma triglyceride levels in founder line 3703 animals were similar to those reported for the 3707 founder line mice.
A transgenic construct containing the human apolipoprotein C-III gene (including 2.5 kb 5' and 1.1 kb 3' flanking sequences) was injected into the male pronucleus of fertilized eggs (both from (C57BL/6J x CBA/J)F1 mice). The resulting animals were bred to (C57BL/6J x CBA/J)F1 to establish founder line 3707. Transgenic mice were bred to wildtype siblings for many generations prior to arrival at The Jackson Laboratory. The Donating Investigator reports that due to a possible transcribing error founder line 3703 (imported by The Jackson Laboratory) is most likely the published founder line 3707, and that plasma triglyceride levels in founder line 3703 animals were similar to those reported for the 3707 founder line mice. The Donating Investigator reports that physical location of transgene insertion(s) are unknown, but given the high expression of the transgene, multiple copies are likely.
Expressed Gene | APOC3, apolipoprotein C3, human |
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Site of Expression |
Allele Name | transgene insertion 3707, Jan L Breslow |
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Allele Type | Transgenic (Inserted expressed sequence, Humanized sequence) |
Allele Synonym(s) | HuCIII Tg |
Gene Symbol and Name | Tg(APOC3)3707Bres, transgene insertion 3707, Jan L Breslow |
Gene Synonym(s) | |
Promoter | APOC3, apolipoprotein C3, human |
Expressed Gene | APOC3, apolipoprotein C3, human |
Strain of Origin | (C57BL/6J x CBA/J)F1 |
Chromosome | UN |
Molecular Note | Male pronuclei of fertilized oocytes were microinjected with a transgenic construct comprising the human apolipoprotein C-III gene, including 2.5 kb of 5' and 1.1 kb of 3' flanking sequence. Mice of this line express the transgene at high levels in the liver and secondarily in the intestine. |
Mutations Made By | Dr. Jan Breslow, Rockefeller University |
When maintaining a live colony, hemizygous mice are bred to wildtype siblings or to B6CBAF1/J (Stock No. 100011).
When using the human apoC-III Tg mouse strain in a publication, please cite the originating article(s) and include JAX stock #006907 in your Materials and Methods section.
Service/Product | Description | Price |
---|---|---|
Hemizygous or non-carrier for Tg(APOC3)3707Bres |
Frozen Mouse Embryo | B6;CBA-Tg(APOC3)3707Bres/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(APOC3)3707Bres/J Frozen Embryo | $2595.00 |
Frozen Mouse Embryo | B6;CBA-Tg(APOC3)3707Bres/J Frozen Embryo | $3373.50 |
Frozen Mouse Embryo | B6;CBA-Tg(APOC3)3707Bres/J Frozen Embryo | $3373.50 |
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