B6;CBA-Tg(APOC3)3707Bres/J

Stock number: 006907
Product name: human apoC-III Tg
Research areas: Cardiovascular Research, Diabetes and Obesity Research, Metabolism Research, Research Tools

Description

Hemizygous mice for this transgene have severe plasma hypertriglyceridemia and significantly increased plasma cholesterol.

Detailed description

Mice hemizygous for this "human apo CIII" transgene are viable and fertile. This high expressor founder line (3703) exhibits transgene expression primarily in the liver with some intestinal expression as well. Hemizygous mice have severe plasma hypertriglyceridemia and significantly increased plasma cholesterol. However, resistance to insulin-mediated glucose uptake or hyperinsulinemia are not observed. This high expressor human apo CIII transgenic strain may be useful in studying lipid metabolism, very low density lipoproteins (VLDL), hypertriglyceridemia, coronary heart disease, and/or atherosclerosis. The Donating Investigator reports that due to a possible transcribing error founder line 3703 (imported by The Jackson Laboratory in 2007) is most likely the published founder line 3707, and that plasma triglyceride levels in founder line 3703 animals were similar to those reported for the 3707 founder line mice.

Development

A transgenic construct containing the human apolipoprotein C-III gene (including 2.5 kb 5' and 1.1 kb 3' flanking sequences) was injected into the male pronucleus of fertilized eggs (both from (C57BL/6J x CBA/J)F1 mice). The resulting animals were bred to (C57BL/6J x CBA/J)F1 to establish founder line 3707. Transgenic mice were bred to wildtype siblings for many generations prior to arrival at The Jackson Laboratory. The Donating Investigator reports that due to a possible transcribing error founder line 3703 (imported by The Jackson Laboratory) is most likely the published founder line 3707, and that plasma triglyceride levels in founder line 3703 animals were similar to those reported for the 3707 founder line mice. The Donating Investigator reports that physical location of transgene insertion(s) are unknown, but given the high expression of the transgene, multiple copies are likely.

Allele

Symbol: Tg(APOC3)3707Bres
Name: transgene insertion 3707, Jan L Breslow
MGI accession ID: MGI:3703618
Generation method: Transgenic
Attributes: Inserted expressed sequence; Humanized sequence
Marker symbol: Tg(APOC3)3707Bres
Marker name: transgene insertion 3707, Jan L Breslow
Chromosome: UN
Strain of origin: (C57BL/6J x CBA/J)F1
Expressed genes: APOC3,apolipoprotein C3,human
Molecular note: Male pronuclei of fertilized oocytes were microinjected with a transgenic construct comprising the human apolipoprotein C-III gene, including 2.5 kb of 5' and 1.1 kb of 3' flanking sequence. Mice of this line express the transgene at high levels in the liver and secondarily in the intestine.